Abstract:
:Vacuolar H(+)-PPase, a membrane bound proton-translocating pyrophosphatase found in various species including plants, some protozoan and prokaryotes, has been demonstrated to be localized to the vacuolar membrane in plants. Using a GUS reporter system and a green fluorescent protein (GFP) fusion protein, we investigated the tissue distribution and the subcellular localization, respectively, of a novel type H(+)-PPase encoded by AVP2/AVPL1 identified in the Arabidopsis thaliana genome. We showed that AVP2/AVPL1 is highly expressed at the trichome and the filament of stamen. Furthermore, the fluorescence of GFP-tagged AVP2/AVPL1 showed small dot-like structures that were observed throughout the cytoplasm of various Arabidopsis cells under a fluorescent microscope. The distribution of this dot-like fluorescent pattern was apparently affected by a treatment with brefeldin A. Moreover, we demonstrated that most dot-like fluorescent structures colocalized with a Golgi resident protein. These findings suggest that this novel type H(+)-PPase resides on the Golgi apparatus rather than the vacuolar membrane.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mitsuda N,Enami K,Nakata M,Takeyasu K,Sato MHdoi
10.1016/s0014-5793(00)02400-5keywords:
subject
Has Abstractpub_date
2001-01-12 00:00:00pages
29-33issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(00)02400-5journal_volume
488pub_type
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