Abstract:
:Myotonic dystrophy protein kinase (DMPK) is a serine-threonine protein kinase encoded by the myotonic dystrophy (DM) locus on human chromosome 19q13.3. It is a close relative of other kinases that interact with members of the Rho family of small GTPases. We show here that the actin cytoskeleton-linked GTPase Rac-1 binds to DMPK, and coexpression of Rac-1 and DMPK activates its transphosphorylation activity in a GTP-sensitive manner. DMPK can also bind Raf-1 kinase, the Ras-activated molecule of the MAP kinase pathway. Purified Raf-1 kinase phosphorylates and activates DMPK. The interaction of DMPK with these distinct signals suggests that it may play a role as a nexus for cross-talk between their respective pathways and may partially explain the remarkable pleiotropy of DM.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Shimizu M,Wang W,Walch ET,Dunne PW,Epstein HFdoi
10.1016/s0014-5793(00)01692-6keywords:
subject
Has Abstractpub_date
2000-06-23 00:00:00pages
273-7issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01692-6journal_volume
475pub_type
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