Abstract:
:The Pep12 protein of Saccharomyces cerevisiae is a member of the syntaxin family thought to function as target membrane receptor (t-SNARE) for vesicular intermediates travelling between the Golgi apparatus and the vacuole. Exploiting the temperature-sensitive growth phenotype of pep12 deletion strains, we identified VAM3 as a multicopy suppressor. Vam3p is another syntaxin-related protein which on high expression restored vacuole acidification of pep12 null mutants and effectively suppressed their sorting and maturation defects of vacuolar hydrolases. We conclude that Vam3p acts either as a bypass suppressor or by functionally replacing Pep12p at an endosomal, prevacuolar compartment.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Götte M,Gallwitz Ddoi
10.1016/s0014-5793(97)00575-9subject
Has Abstractpub_date
1997-07-07 00:00:00pages
48-52issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00575-9journal_volume
411pub_type
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