The translocation mechanism of P-glycoprotein.

abstract：:The structure of the Ala38 variant of yeast iso-1-cytochrome c, in which the previously unchanged Arg38 has been replaced, has been characterised by NMR. The NMR data indicate that the structure of the Ala38 variant is very similar to that of the wild type protein. In particular, the heme environment and interactions ...

journal_title：FEBS letters

authors： Thurgood AG,Pielak GJ,Cutler RL,Davies AM,Greenwood C,Mauk AG,Smith M,Williamson DJ,Moore GR

更新日期：1991-06-24 00:00:00

abstract：:The red cell membrane is comprised of a lipid bilayer studded with transmembrane proteins, and laminated by a protein network, the membrane skeleton, at the surface of the inner monolayer. The erythrocyte owes its mechanical properties to the membrane skeleton. Hereditary spherocytosis, hereditary elliptocytosis or po...

journal_title：FEBS letters

authors： Delaunay J

更新日期：1995-08-01 00:00:00

abstract：:The effects of insulin and IGF-I on the cell surface quantities of GLUT1, GLUT3 and GLUT4 glucose transporters in L6 myotubes were determined with the exofacial bis-mannose phololabel (ATB-BMPA). In basal cells, an equal molar quantity of each transporter isoform was found at the cell surface. Insulin stimulated the t...

journal_title：FEBS letters

authors： Wilson CM,Mitsumoto Y,Maher F,Klip A

更新日期：1995-07-10 00:00:00

abstract：:There are two common forms of NRH-quinone oxidoreductase 2 (NQO2) in the human population resulting from SNP rs1143684. One has phenylalanine at position 47 (NQO2-F47) and the other leucine (NQO2-L47). Using recombinant proteins, we show that these variants have similar steady state kinetic parameters, although NQO2-L...

journal_title：FEBS letters

authors： Megarity CF,Gill JR,Caraher MC,Stratford IJ,Nolan KA,Timson DJ

更新日期：2014-05-02 00:00:00

abstract：:Phalloidin and fluorescently labeled phalloidin analogs are established reagents to stabilize and mark actin filaments for the investigation of acto-myosin interactions. In the present study, we employed transient and steady-state kinetic measurements as well as in vitro motility assays to show that phalloidin perturb...

journal_title：FEBS letters

authors： Diensthuber RP,Müller M,Heissler SM,Taft MH,Chizhov I,Manstein DJ

更新日期：2011-03-09 00:00:00

abstract：:Pigeon liver malic enzyme (EC 1.1.1.40) has a double dimer quaternary structure. The NADP+ analogs, aminopyridine adenine dinucleotide phosphate and nicotinamide-1,N6-ethenoadenosine dinucleotide phosphate, bind to the enzyme anti-cooperatively. In the presence of non-cooperative competing ligand NADP+, the binding pa...

journal_title：FEBS letters

authors： Lee HJ,Chang GG

更新日期：1990-12-17 00:00:00

abstract：:Sequence comparisons of 5 S and other ribosomal RNAs by segments can be useful in understanding anomalous primary and secondary structures and in assessing phylogenetic relationships. In a segmented analysis, the 5'-half of the Chlamydomonas reinhardii chloroplast 5 S ribosomal RNA is found to have a very close sequen...

journal_title：FEBS letters

authors： Delihas N

更新日期：1987-09-14 00:00:00

abstract：:The MDM2 oncoprotein is overexpressed in many human tumors and cancers. MDM2 functions as an E3 ligase for p53 and for itself. MDM2 also interacts with the retinoblastoma protein (RB) and the transcription factor E2F1 to promote cell cycle S-phase entry. Here, we report that MDM2 protein expression is cell cycle-regul...

journal_title：FEBS letters

authors： Gu L,Ying H,Zheng H,Murray SA,Xiao ZX

更新日期：2003-06-05 00:00:00

abstract：:In Clostridium thermoaceticum, the synthesis of acetyl-CoA from methyl tetrahydrofolate occurs via a series of enzymatic reactions involving methyl transferase, corrinoid/Fe-S protein (corrinoid), carbon monoxide dehydrogenase (CODH) and ferredoxin. We have investigated the possibility of one or more of these proteins...

journal_title：FEBS letters

authors： Shanmugasundaram T,Sundaresh CS,Kumar GK

更新日期：1993-07-12 00:00:00

abstract：:Proline residues are rarely found in the three most C-terminal positions of bacterial signal peptides, and have never been found in position +1 immediately following the cleavage site. It was recently shown that a Pro+1 mutation in the E. coli maltose binding protein precursor not only prevents cleavage of the signal ...

journal_title：FEBS letters

authors： Nilsson I,von Heijne G

更新日期：1992-03-16 00:00:00

abstract：:CD133 is a widely used cell surface marker of cancer stem cells that plays an important role in tumor initiation and metastasis. Increasing evidence shows that CD133 is secreted to the extracellular space. However, the underlying mechanisms of CD133 secretion remain largely unknown. In this study, we report that secre...

journal_title：FEBS letters

authors： Li Y,Shi D,Yang F,Chen X,Xing Y,Liang Z,Zhuang J,Liu W,Gong Y,Jiang J,Wei Y

更新日期：2019-04-01 00:00:00

abstract：:cDNAs encoding aquaporins PIP1;1, PIP2;1, and TIP1;1 were isolated from Mimosa pudica (Mp) cDNA library. MpPIP1;1 exhibited no water channel activity; however, it facilitated the water channel activity of MpPIP2;1 in a phosphorylation-dependent manner. Mutagenesis analysis revealed that Ser-131 of MpPIP1;1 was phospho...

journal_title：FEBS letters

authors： Temmei Y,Uchida S,Hoshino D,Kanzawa N,Kuwahara M,Sasaki S,Tsuchiya T

更新日期：2005-08-15 00:00:00

abstract：:Contrary to previously published data, we have found that in mammalian skeletal muscles, phosphoglycerate mutase (PGM) is organized in a regular, striated fashion within the sarcomere. In the absence of the enzyme effectors, PGM localizes mainly at the M-line, but under conditions typical for contracting muscle, the e...

journal_title：FEBS letters

authors： Kowalski W,Gizak A,Rakus D

更新日期：2009-06-18 00:00:00

abstract：:The possible relationship between pyridoxal phosphate-dependent ornithine decarboxylase (ODC) activity and glucose-6-phosphate dehydrogenase (G6PD) activity has been studied in the osteoblasts of the growth-plate of metatarsals of rats fed a pyridoxine-deficient diet, which caused depressed G6PD levels. The G6PD activ...

journal_title：FEBS letters

authors： Dodds RA,Dunham J,Bitensky L,Chayen J

更新日期：1986-05-26 00:00:00

abstract：:A peptide reproducing the G-H loop amino acid sequence of foot-and-mouth disease virus VP1 protein was fused to the solvent-exposed C-terminus of the bacteriophage P22 tailspike protein [Carbonell and Villaverde (1996) Gene, in press], a homotrimeric polypeptide with a strong beta-helical structure. This fusion does n...

journal_title：FEBS letters

authors： Carbonell X,Benito A,Villaverde A

更新日期：1996-11-18 00:00:00

abstract：:Fibroblast growth factor 11 (FGF11) is an intracellular FGF. Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia-inducible factor-1α (HIF-1α) as an int...

journal_title：FEBS letters

authors： Lee KW,Yim HS,Shin J,Lee C,Lee JH,Jeong JY

更新日期：2017-01-01 00:00:00

abstract：:In addition to the selective amplification of cDNA from total RNA by the PCR method, the distinctive properties of ferredoxin-expressing colonies can be used for cloning a ferredoxin cDNA. This strategy for cloning and expressing cDNA in E. coli was applied to a sheep adreno-ferredoxin. The expressed sheep ferredoxin ...

journal_title：FEBS letters

authors： Matsuo Y,Tsujita M,Mizoguchi K,Ichikawa Y

更新日期：1992-04-20 00:00:00

abstract：:When MCF-7 cells were treated with 17 beta-estradiol, dexamethasone, or promegestone at 37 degrees C, the KD of receptors for their cognate ligands was found to decrease as compared to that measured at 2 degrees C. Cell incubation with hormone at 37 degrees C did not affect the Bmax of glucocorticoid and progesterone ...

journal_title：FEBS letters

authors： Rossini GP,Malaguti C

更新日期：1995-12-04 00:00:00

abstract：:We employed cDNA representational difference analysis to identify new genes that are upregulated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in a human hepatoblastoma cell line HepG2. We isolated several TCDD-responsive cDNAs. Sequence analysis revealed that one of them encodes E16/CD98LC/hLAT1, an integral membrane...

journal_title：FEBS letters

authors： Sarkar D,Kambe F,Hirata A,Iseki A,Ohmori S,Seo H

更新日期：1999-12-03 00:00:00

abstract：:Wild-type trypanosomal triosephosphate isomerase (wtTIM) is a very tight dimer. The interface residue His-47 of wtTIM has been mutated into an asparagine. Ultracentrifugation data show that this variant (H47N) only dimerises at protein concentrations above 3 mg/ml. H47N has been characterised at a protein concentratio...

journal_title：FEBS letters

authors： Borchert TV,Zeelen JP,Schliebs W,Callens M,Minke W,Jaenicke R,Wierenga RK

更新日期：1995-07-03 00:00:00

abstract：:Different bioinformatics methods illuminate different aspects of protein function, from specific catalytic activities to broad functional categories. Here, a triple-pronged approach to predict function for a domain of unknown function, DUF2086, is applied. Distant homology to characterised enzymes and conservation of ...

journal_title：FEBS letters

authors： Mello LV,Rigden DJ

更新日期：2012-11-02 00:00:00

abstract：:Mutation of Thr90 to Ala has a profound effect on bacteriorhodopsin properties. T90A shows about 20% of the proton pumping efficiency of wild type, once reconstituted into liposomes. Mutation of Thr90 influences greatly the Schiff base/Asp85 environment, as demonstrated by altered lambda(max) of 555 nm and pK(a) of As...

journal_title：FEBS letters

authors： Perálvarez A,Barnadas R,Sabés M,Querol E,Padrós E

更新日期：2001-11-23 00:00:00

abstract：:The triplex structure in vitro is well established; however, no direct evidence has been available concerning its existence in the cell. Using the direct chemical probing here we show that the triplex H structure can exist in E. coli cells at acidic intracellular pH values; this structure differs in some details from ...

journal_title：FEBS letters

authors： Karlovsky P,Pecinka P,Vojtiskova M,Makaturova E,Palecek E

更新日期：1990-11-12 00:00:00

abstract：:The complete amino acid sequence of porcine muscarinic acetylcholine receptor III has been deduced by cloning and sequencing the genomic DNA. The antagonist binding properties of muscarinic acetylcholine receptor III expressed from the cloned DNA in Xenopus oocytes correspond most closely to those of the pharmacologic...

journal_title：FEBS letters

authors： Akiba I,Kubo T,Maeda A,Bujo H,Nakai J,Mishina M,Numa S

更新日期：1988-08-01 00:00:00

abstract：:Expression of androgen receptor (AR) in prostate epithelial cells is thought to regulate cell proliferation, differentiation, and survival. However, the molecular mechanisms remain unclear. We report that re-expression of AR in PC-3 human prostate cancer cell line resulted in upregulation of IFI16 protein, a negative ...

journal_title：FEBS letters

authors： Alimirah F,Chen J,Xin H,Choubey D

更新日期：2006-03-06 00:00:00

abstract：:We analyzed the expression of aquaporins (AQPs) in the small intestine to elucidate their functions, and found that AQP9, which had not previously been detected there, is present in duodenum, jejunum, and ileum. AQP9 is expressed in colon as well, but not in stomach. Also, its expression in these intestinal sections i...

journal_title：FEBS letters

authors： Okada S,Misaka T,Matsumoto I,Watanabe H,Abe K

更新日期：2003-04-10 00:00:00

abstract：:Anandamide loses its cannabimimetic activities upon hydrolysis to arachidonic acid and ethanolamine. So far the anandamide hydrolyzing activity widely distributed in mammalian organs has been attributed exclusively to an enzyme referred to as anandamide amidohydrolase with an optimum pH around 9. We found another enzy...

journal_title：FEBS letters

authors： Ueda N,Yamanaka K,Terasawa Y,Yamamoto S

更新日期：1999-07-09 00:00:00

abstract：:A human insulinoma cDNA (PC2) that encodes a protein homologous to the Kex2/subtilisin-like proteinases has recently been described [1990, J. Biol. Chem. 265, 2997-3000]. In order to characterise the associated proteinase activity, mRNA encoding PC2 was synthesised in vitro and microinjected into Xenopus oocytes. The ...

journal_title：FEBS letters

authors： Shennan KI,Smeekens SP,Steiner DF,Docherty K

更新日期：1991-06-24 00:00:00

abstract：:The complete amino acid sequence of a mammalian acetylcholinesterase from fetal bovine serum (FBS AChE) is presented. This enzyme has a high degree of sequence identity with other cholinesterases, liver carboxyesterases, esterase-6, lysophospholipase, and thyroglobulin. The locations of 191 amino acids in 10 regions o...

journal_title：FEBS letters

authors： Doctor BP,Chapman TC,Christner CE,Deal CD,De La Hoz DM,Gentry MK,Ogert RA,Rush RS,Smyth KK,Wolfe AD

更新日期：1990-06-18 00:00:00

abstract：:A cDNA clone (pUP-23) corresponding to a member of a protein family that includes inhibitors of trypsin and of heterologous alpha-amylases has been selected from a library derived from developing barley endosperm and its sequence has been determined. A stretch of 95 nucleotides that included the signal peptide and the...

journal_title：FEBS letters

authors： Lázaro A,Rodriguez-Palenzuela P,Maraña C,Carbonero P,Garcia-Olmedo F

更新日期：1988-10-24 00:00:00