Cloning and characterization of a novel human pH-dependent organic cation transporter, OCTN1.

abstract：:Human ovarian clear cell carcinoma cell line (transferrin (Tf)-non-producer), HAC 2, cells were adapted to grow in chemically defined synthetic medium when the cells were cultured with medium containing 10 micrograms/ml of insulin at least for 6 months. They synthesized and secreted constantly the 80 kDa protein immun...

journal_title：FEBS letters

authors： Ohkawa K,Takada K,Takizawa N,Hatano T,Tsukada Y,Matsuda M

更新日期：1990-09-17 00:00:00

abstract：:The concentration of insulin that produces half-maximal stimulation of glycolysis by stripped soleus muscle preparations is markedly increased by the adenosine analogues, 2-chloroadenosine and N6-phenylisopropyladenosine, but is markedly decreased by the methyl xanthine analogue, 8-phenyltheophylline. 2-Chloroadenosin...

journal_title：FEBS letters

authors： Budohoski L,Challiss RA,McManus B,Newsholme EA

更新日期：1984-02-13 00:00:00

abstract：:The gene for human tetranectin was isolated from a genomic library with a mixture of degenerate oligonucleotide probes. The gene is about 12 kbp and contains two intervening sequences. The gene encodes a protein of 202 amino acid residues, with a signal peptide of 21 amino acid residues, followed by the tetranectin se...

journal_title：FEBS letters

authors： Berglund L,Petersen TE

更新日期：1992-08-31 00:00:00

abstract：:Two new three-disulfide intermediates have been found to be populated in the oxidative folding pathway of bovine pancreatic ribonuclease A at a low temperature (15 degrees C). These intermediates, des-[26-84] and des-[58-110], possess all but one of the four native disulfide bonds and have a stable tertiary structure,...

journal_title：FEBS letters

authors： Welker E,Narayan M,Volles MJ,Scheraga HA

更新日期：1999-11-05 00:00:00

abstract：:The covalent binding of pyridoxal 5'-phosphate (PLP) to human serum albumin (HSA) is important in the regulation of PLP metabolism. In plasma, PLP is bound to HSA at a single high-affinity and at two or more nonspecific sites. To characterize the primary PLP binding site, HSA was incubated with [3H] PLP, and the Schif...

journal_title：FEBS letters

authors： Bohney JP,Fonda ML,Feldhoff RC

更新日期：1992-02-24 00:00:00

abstract：:The quadruple mutation of the whole basic cluster, K74KKK77 conserved in the catalytic subunits of protein kinase CK2 and implicated in substrate recognition, not only abolishes inhibition by heparin but even induces with some peptide substrates an up to 5-fold stimulation by heparin in the 0.5-5 micrograms/ml concent...

journal_title：FEBS letters

authors： Vaglio P,Sarno S,Marin O,Meggio F,Issinger OG,Pinna LA

更新日期：1996-02-12 00:00:00

abstract：:Calf chymosin molecules exist in the two alternative structural forms: the first one has S1 and S3 binding pockets occluded by its own Tyr77 residue (the self-inhibited form); the second has these pockets free for a substrate binding (the active form). The preliminary incubation of the enzyme with a pentapeptide corre...

journal_title：FEBS letters

authors： Gustchina E,Rumsh L,Ginodman L,Majer P,Andreeva N

更新日期：1996-01-22 00:00:00

abstract：:The dependence of protein splicing on conserved residues of the Cne PRP8 intein was assessed by alanine scanning mutagenesis in a foreign protein context. Corroboration was obtained for the involvement of residues at the splice junctions and of the conserved threonine and histidine of motif B. Five additional residues...

journal_title：FEBS letters

authors： Pearl EJ,Tyndall JD,Poulter RT,Wilbanks SM

更新日期：2007-06-26 00:00:00

abstract：:The structure of GE82832, a translocation inhibitor produced by a soil microorganism, is shown to be highly related to that of dityromycin, a bicyclodecadepsipeptide antibiotic discovered long ago whose characterization had never been pursued beyond its structural elucidation. GE82832 and dityromycin were shown to int...

journal_title：FEBS letters

authors： Brandi L,Maffioli S,Donadio S,Quaglia F,Sette M,Milón P,Gualerzi CO,Fabbretti A

更新日期：2012-09-21 00:00:00

abstract：:We review the evidence for H+ translocation by reconstituted cytochrome c oxidase; attention is paid to those aspects which we feel most open to criticism. Possible alternative hypotheses are assessed, with regard to experiments carried out to test them directly and with regard to published data. We conclude that, whi...

journal_title：FEBS letters

authors： Casey RP,Azzi A

更新日期：1983-04-18 00:00:00

abstract：:Mona is an SH3 and SH2 domain-containing adapter molecule that is induced during monocytic differentiation. Here we have first shown that M-CSFR is the major Mona partner in M-CSF signaling, the interaction being mediated through tyrosine 697 of the receptor. Next we asked whether Mona expression would alter the Ras/M...

journal_title：FEBS letters

authors： Bourgin C,Bourette R,Mouchiroud G,Arnaud S

更新日期：2000-09-01 00:00:00

abstract：:The failure of insulin-regulated recruitment of the GLUT4 glucose transporter in cardiac muscle of obese Zucker rats is associated with alterations of the subcellular distribution of the small-molecular-mass GTP-binding protein rab4A. Here, we show by subcellular fractionation and Western blotting a translocation of t...

journal_title：FEBS letters

authors： Uphues I,Chern Y,Eckel J

更新日期：1995-12-18 00:00:00

abstract：:Addition of hydrocortisone to the rat mammary gland myoepithelial cell line, G4.2.3, induces the synthesis and secretion of alpha 2-macroglobulin. Interleukin-1 beta, interleukin-6 and transforming growth factor-beta synergize with hydrocortisone, increasing the synthesis of alpha 2-macroglobulin 2- to 4-fold, althoug...

journal_title：FEBS letters

authors： Warburton MJ,Gusterson BA,O'Hare MJ

更新日期：1993-10-11 00:00:00

abstract：:Interrogation of the Human Genome data for sequences related to the von Willebrand factor A-domain module identified a previously unreported 4.1 kb full-length cDNA that is predicted to encode a new member of the collagen superfamily of extracellular matrix proteins, collagen XXI. The domain organization of collagen X...

journal_title：FEBS letters

authors： Fitzgerald J,Bateman JF

更新日期：2001-09-14 00:00:00

abstract：:A human placenta cDNA expression library was screened for genes inducing flat reversion when transfected into a v-K-ras-transformed NIH3T3 cell line, DT. One such gene was found to encode a Kunitz-type serine protease inhibitor, tissue factor pathway inhibitor-2 (TFPI-2). While the TFPI-2 mRNA can be detected in norma...

journal_title：FEBS letters

authors： Izumi H,Takahashi C,Oh J,Noda M

更新日期：2000-09-08 00:00:00

abstract：:In our study, transcriptome microarrays are used to identify differentially expressed miRNAs and mRNAs in cervical cancer specimens. We find that microRNA-145 (miR-145) expression is significantly decreased in cervical cancer tissues and cell lines, and is associated with advanced cancer stages, large tumor size and m...

journal_title：FEBS letters

authors： Ye C,Sun NX,Ma Y,Zhao Q,Zhang Q,Xu C,Wang SB,Sun SH,Wang F,Li W

更新日期：2015-03-12 00:00:00

abstract：:Mature beta-hexosaminidase A has been found associated to the external leaflet of plasma membrane of cultured fibroblasts. The plasma membrane association of beta-hexosaminidase A has been directly determined by cell surface biotinylation followed by affinity chromatography purification of the biotinylated proteins, a...

journal_title：FEBS letters

authors： Mencarelli S,Cavalieri C,Magini A,Tancini B,Basso L,Lemansky P,Hasilik A,Li YT,Chigorno V,Orlacchio A,Emiliani C,Sonnino S

更新日期：2005-10-24 00:00:00

abstract：:Crosslinking of proteins by tissue transglutaminase (tTG) is enhanced in amyloid (Abeta) deposits characteristic of Alzheimer's disease and sporadic inclusion body myositis. Small heat shock proteins (sHsps) also occur in amyloid deposits. We here report the substrate characteristics for tTG of six sHsps. Hsp27, Hsp20...

journal_title：FEBS letters

authors： Boros S,Kamps B,Wunderink L,de Bruijn W,de Jong WW,Boelens WC

更新日期：2004-10-08 00:00:00

abstract：:In order to elucidate the molecular mechanisms responsible for the specific lipid-protein interactions, we have undertaken structural and idiotypic analyses of a monoclonal antibody, PS4A7, which binds specifically to phosphatidylserine (PS). Here we showed that one of the anti-idiotypic monoclonal antibodies raised a...

journal_title：FEBS letters

authors： Reza F,Igarashi K,Tokita S,Asai K,Aoki J,Asaoka Y,Umeda M,Inoue K

更新日期：1994-02-21 00:00:00

abstract：:Hepatocyte tight junctional permeability has been shown to be regulated by hormones that exert their effects via phospholipase C activation. However, the precise transduction pathway involved in this effect is not known. The present study has employed the selective inhibitor of microsomal Ca2+ sequestration, 2,5-di(te...

journal_title：FEBS letters

authors： Llopis J,Kass GE,Duddy SK,Farell GC,Gahm A,Orrenius S

更新日期：1991-03-11 00:00:00

abstract：:Previous studies using retinal analogs (Wada, A., Sakai, M., Kinumi, T., Tsujimoto, K., Yamauchi, M. and Ito, M. (1994) J. Org. Chem. 59, 6992-6997; Wada, A., Sakai, M., Imamoto, Y., Shichida., Y., Yoshizawa, T. and Ito, M. (1993) Chem. Pharm. Bull. 41, 793-795) revealed that both retinochrome and visual pigments shar...

journal_title：FEBS letters

authors： Sakamoto M,Wada A,Akai A,Ito M,Goshima T,Takahashi T

更新日期：1998-09-04 00:00:00

abstract：:Classically, alpha-1,4-glucan synthases have been divided into two families, animal/fungal glycogen synthases (GS) and bacterial/plant starch synthases (G(S)S), according to differences in sequence, sugar donor specificity and regulatory mechanisms. Detailed sequence analysis, predicted secondary structure comparison ...

journal_title：FEBS letters

authors： Cid E,Geremia RA,Guinovart JJ,Ferrer JC

更新日期：2002-09-25 00:00:00

abstract：:Yeast cells lacking the metacaspase-encoding gene YCA1 (Deltayca1) were compared with wild-type (WT) cells with respect to the occurrence, nature and time course of acetic-acid triggered death. We show that Deltayca1 cells undergo programmed cell death (PCD) with a rate lower than that of the WT and that PCD in WT cel...

journal_title：FEBS letters

authors： Guaragnella N,Pereira C,Sousa MJ,Antonacci L,Passarella S,Côrte-Real M,Marra E,Giannattasio S

更新日期：2006-12-22 00:00:00

abstract：:OmpC, a surface antigen of Salmonella typhi was crystallized after several attempts, using PEG 3350. Well shaped hexagonal crystals were grown from vapor diffusion method using octyl glucoside and C12E9 as detergents. Crystals are sensitive to X-ray and diffract weakly up to 7 A. Porin isoforms, due to the bound lipop...

journal_title：FEBS letters

authors： Arockiasamy A,Krishnaswamy S

更新日期：1999-06-25 00:00:00

abstract：:In yeast, three AAA superfamily metalloproteases (Yme1p, Afg3p and Rca1p) are localized to the mitochondrial inner membrane where they perform roles in the assembly and turnover of the respiratory chain complexes. We have investigated the function of the proposed human orthologue of yeast Yme1p, encoded by the YME1L g...

journal_title：FEBS letters

authors： Shah ZH,Hakkaart GA,Arku B,de Jong L,van der Spek H,Grivell LA,Jacobs HT

更新日期：2000-08-04 00:00:00

abstract：:Culturing human fibroblasts in a three-dimensional collagen matrix leads to a reduction of collagen I by more than 90%, both on the level of mRNA steady-state as well as protein. In order to differentiate changes in de novo transcription and posttranscriptional control, nuclear run on assays and pulse/chase experiment...

journal_title：FEBS letters

authors： Eckes B,Mauch C,Hüppe G,Krieg T

更新日期：1993-03-01 00:00:00

abstract：:Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding of paraoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase...

journal_title：FEBS letters

authors： Heldman E,Balan A,Horowitz O,Ben-Zion S,Torten M

更新日期：1985-01-28 00:00:00

abstract：:A human liver cDNA clone which encodes the UDP-glucose pyrophosphorylase was isolated by complementation of a bacterial galU mutant. The deduced amino acid sequence of the human enzyme comprised 508 amino acids with a calculated molecular mass of 56,950. The human enzyme significantly resembles those of potato tuber a...

journal_title：FEBS letters

authors： Peng HL,Chang HY

更新日期：1993-08-23 00:00:00

abstract：:It was found for RNases of different specificities that changes in the free energy for substrate-enzyme binding induced by variations in the nucleotide base structure are accompanied by proportional changes in kcat/Km. This was considered to be a consequence of the strain in the enzyme-substrate complex. ...

journal_title：FEBS letters

authors： Yakovlev GI,Bocharov AL,Moiseyev GP

更新日期：1984-10-01 00:00:00

abstract：:We have studied the effects of 'hydrophobic mismatch' between a poly-Leu transmembrane helix (TMH) and the ER membrane using a glycosylation mapping approach. The simplest interpretation of our results is that the lumenal end of the TMH is located deeper in the membrane for both short (negative mismatch) and long (pos...

journal_title：FEBS letters

authors： Monné M,von Heijne G

更新日期：2001-05-11 00:00:00