Abstract:
:The role of adenosine 5'-triphosphate (ATP) and P2Y(1) nucleotide receptor in potentiating agrin-induced acetylcholine receptor (AChR) aggregation is being demonstrated in a co-culture system of NG108-15 cell, a mouse neuroblastoma X rat glioma hybrid cell line that resembles spinal motor neuron, with C2C12 myotube. In the co-cultures, antagonized P2Y(1) receptors showed a reduction in NG108-15 cell-induced AChR aggregation. Parallel to this observation, cultured NG108-15 cell secreted ATP into the conditioned medium in a time-dependent manner. Enhancement of ATP release from the cultured NG108-15 cells by overexpression of active mutants of small GTPases increased the aggregation of AChRs in co-culturing with C2C12 myotubes. In addition, ecto-nucleotidase was revealed in the co-culture, which rapidly degraded the applied ATP. These results support the notion that ATP has a role in directing the formation of post-synaptic apparatus in vertebrate neuromuscular junctions.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ling KK,Siow NL,Choi RC,Tsim KWdoi
10.1016/j.febslet.2005.03.054keywords:
subject
Has Abstractpub_date
2005-04-25 00:00:00pages
2469-74issue
11eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00400-Xjournal_volume
579pub_type
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