Activation of the Na+/K+-ATPase by interleukin-2.

abstract：:Recombinant expression of the large alternansucrase (2057 amino acids) was hindered in E. coli due to poor enzyme solubility and protein degradation. The effects of deletions of the alternansucrase C-terminal CW-like and APY repeated motifs on enzyme solubility and specificity were investigated. A truncated variant de...

journal_title：FEBS letters

authors： Joucla G,Pizzut S,Monsan P,Remaud-Simeon M

更新日期：2006-02-06 00:00:00

abstract：:Several analogues of heat-stable enterotoxins (STh and STp) produced by enterotoxigenic Escherichia coli were synthesized. Peptides (STh[6-18] and STp[5-17]) consisting of 13 amino acid residues from the Cys residue near the N-terminus to the Cys residue near the C-terminus and linked by three disulfide bonds had the ...

journal_title：FEBS letters

authors： Yoshimura S,Ikemura H,Watanabe H,Aimoto S,Shimonishi Y,Hara S,Takeda T,Miwatani T,Takeda Y

更新日期：1985-02-11 00:00:00

abstract：:A Ca2+ indicator arsenazo III was used to demonstrate calcium uptake activity of symbiosomes and the peribacteroid membrane (PBM) vesicles isolated from broad bean root nodules and placed in the medium containing ATP and Mg2+ ions. This process was shown to be rapidly stopped by vanadate, completely reversed in the pr...

journal_title：FEBS letters

authors： Andreev IM,Dubrovo PN,Krylova VV,Izmailov SF

更新日期：1999-03-19 00:00:00

abstract：:Ballistic transfection, based on cell and tissue bombardment by the tungsten and gold microparticles covered with the gene DNA, was used for the delivery of a bacterial beta-galactosidase and a full-length cDNA copy of the human dystrophin genes into mouse skeletal muscles. CMV-lacZ, SV40-lacZ, LTR-lacZneo and full-le...

journal_title：FEBS letters

authors： Zelenin AV,Kolesnikov VA,Tarasenko OA,Shafei RA,Zelenina IA,Mikhailov VV,Semenova ML,Kovalenko DV,Artemyeva OV,Ivaschenko TE,Evgrafov OV,Dickson G,Baranovand VS

更新日期：1997-09-08 00:00:00

abstract：:Human Tid-1 (hTid-1) is a DnaJ chaperone protein with homology to the Drosophila tumor suppressor Tid56. We report the first case of a tumor-associated mutation at the human TID1 locus, which was identified in the SF767 glioma cell line giving rise to aberrantly high levels of a hTid-1(L) mutant variant. In this study...

journal_title：FEBS letters

authors： Trentin GA,He Y,Wu DC,Tang D,Rozakis-Adcock M

更新日期：2004-12-17 00:00:00

abstract：:In the current work, we report that specific inhibition of Janus tyrosine kinase (JAK3) via NC1153 induces apoptosis of certain leukemia/lymphoma cell lines. Affymetrix microarray profiling following NC1153 treatment unveiled JAK3 dependent survival modulating pathways (p53, TGF-beta, TNFR and ER stress) in Kit225 cel...

journal_title：FEBS letters

authors： Nagy ZS,Ross JA,Rodriguez G,Bader J,Dimmock J,Kirken RA

更新日期：2010-04-16 00:00:00

abstract：:Inositol 1,4,5-trisphosphate (1,4,5-InsP3) was perfused into rat dorsal root ganglion (DRG) neurons by whole-cell patch-clamp electrodes, while measuring the membrane potential. This operation evoked a transient (2-3 min) membrane hyperpolarization of about -15 mV (from -42 mV) followed by a depolarization. The membra...

journal_title：FEBS letters

authors： Wang Z,Ypey DL,Van Duijn B

更新日期：1992-06-15 00:00:00

abstract：:The eye lens protein and chaperonin, alpha-crystallin, was studied by differential scanning microcalorimetry, spectroscopy and size exclusion chromatography. The thermal transition of alpha-crystallin proceeds at Ttrs = 59.8 +/- 0.6 degrees C with an enthalpy change of delta H = 336 +/- 9 kJ per mol subunit. Disagreem...

journal_title：FEBS letters

authors： Gesierich U,Pfeil W

更新日期：1996-09-16 00:00:00

abstract：:The effect of the three platelet-derived growth factor (PDGF) isoforms AA, AB and BB on the induction of the early growth response genes c-fos, egr-1 and c-myc mRNA in vascular smooth muscle cells from rat was compared with their respective mitogenic potency. The three PDGF isoforms strongly stimulated the induction t...

journal_title：FEBS letters

authors： Sachinidis A,Schulte K,Ko Y,Meyer zu Brickwedde MK,Hoppe V,Hoppe J,Vetter H

更新日期：1993-03-22 00:00:00

abstract：:Gibberellins are ent-kaurene-derived diterpenoid phytohormones produced by plants, fungi, and bacteria. The distinct gibberellin biosynthetic pathways in plants and fungi are known, but not that in bacteria. Plants typically use two diterpene synthases to form ent-kaurene, while fungi use only a single bifunctional di...

journal_title：FEBS letters

authors： Morrone D,Chambers J,Lowry L,Kim G,Anterola A,Bender K,Peters RJ

更新日期：2009-01-22 00:00:00

abstract：:Human monoclonal antibodies often display limited thermodynamic and colloidal stabilities. This behavior hinders their production, and places limitations on the development of novel formulation conditions and therapeutic applications. Antibodies are highly diverse molecules, with much of the sequence variation observe...

journal_title：FEBS letters

authors： Rouet R,Lowe D,Christ D

更新日期：2014-01-21 00:00:00

abstract：:Five mouse and human leptin receptors (Ob-R) have recently been identified, a long isoform (Ob-Rb), preferentially expressed in hypothalamus, and 4 short isoforms, Ob-Ra, Ob-Rc, Ob-Rd, and Ob-Re. We have identified a new short isoform in the rat, r-OB-Rf, with 6 C-terminal amino acids and a 3' untranslated region with...

journal_title：FEBS letters

authors： Wang MY,Zhou YT,Newgard CB,Unger RH

更新日期：1996-08-26 00:00:00

abstract：:Proline residues are rarely found in the three most C-terminal positions of bacterial signal peptides, and have never been found in position +1 immediately following the cleavage site. It was recently shown that a Pro+1 mutation in the E. coli maltose binding protein precursor not only prevents cleavage of the signal ...

journal_title：FEBS letters

authors： Nilsson I,von Heijne G

更新日期：1992-03-16 00:00:00

abstract：:LIM-homeodomain (LIM-HD) proteins are evolutionary conserved developmental transcription factors. LIM-HD Lmx1a and Lmx1b orchestrate complex temporal and spatial gene expression of the dopaminergic pathway, and evidence shows they are also involved in adult neuronal homeostasis. In this review, the multiple roles play...

journal_title：FEBS letters

authors： Doucet-Beaupré H,Ang SL,Lévesque M

更新日期：2015-12-21 00:00:00

abstract：:Tau is reversibly hyperphosphorylated in the mouse brain by starvation or cold water swimming. Here, we report tau phosphorylation in the hippocampus of normal mouse after ether anesthesia, known to trigger typical stress reactions. Robust phosphorylation of tau was observed immediately and 10min after ether vapor exp...

journal_title：FEBS letters

authors： Ikeda Y,Ishiguro K,Fujita SC

更新日期：2007-03-06 00:00:00

abstract：:Structural and biochemical studies have begun to elucidate the pathway of RNA silencing that leads to the formation of the RISC complex. The outstanding feature of this pathway is the precise recognition and processing of double-stranded RNA. We present a review of recent structures that illustrate the molecular mecha...

journal_title：FEBS letters

authors： Collins RE,Cheng X

更新日期：2005-10-31 00:00:00

abstract：:Complex I (NADH:ubiquinone oxidoreductase) is the first and largest protein complex of the oxidative phosphorylation. Crystal structures have elucidated the positions of most subunits of bacterial evolutionary origin in the complex, but the positions of the eukaryotic subunits are unknown. Based on the analysis of seq...

journal_title：FEBS letters

authors： Szklarczyk R,Wanschers BF,Nabuurs SB,Nouws J,Nijtmans LG,Huynen MA

更新日期：2011-03-09 00:00:00

abstract：:Yeast genes involved in thiamin pyrophosphate (TPP) synthesis are transcriptionally induced in response to thiamin starvation. In this system, three proteins (Thi2p, Thi3p, and Pdc2p) act as positive regulatory factors. Thi3p is a TPP-binding protein and upregulates THI genes expression when TPP is not bound. We found...

journal_title：FEBS letters

authors： Nosaka K,Onozuka M,Konno H,Akaji K

更新日期：2008-12-10 00:00:00

abstract：:Using specific antisera, the expression of the G protein alpha subunits, G8, G(i1), G(i2), G(i3) and G0, were determined in 3T3-F442A cells during their differentiation to adipocytes in a hormonally defined medium. Differentiation caused distinct increases in the expression of two Gs isoforms and decreases in the expr...

journal_title：FEBS letters

authors： Kilgour E,Anderson NG

更新日期：1993-08-16 00:00:00

abstract：:E. coli GroEL chaperonin monomers, isolated after urea-induced dissociation of GroEL14, undergo cold denaturation below 5 degrees C. Above 5 degrees C, these monomers undergo MgATP-dependent self-assembly. We have demonstrated a conformational transition at 0 degree C induced by interaction of monomeric GroEL with ade...

journal_title：FEBS letters

authors： Lissin NM,Hemmingsen SM

更新日期：1993-06-07 00:00:00

abstract：:Using radiolysis with (32)P enriched phosphate as an internal source of ionizing radiation, the formation of hydroperoxo-ferric complex from oxy-ferrous precursor with a high yield was monitored at 77 K in heme oxygenase (HO) by means of optical absorption spectroscopy. Well-resolved absorption spectra (maxima at 421 ...

journal_title：FEBS letters

authors： Denisov IG,Ikeda-Saito M,Yoshida T,Sligar SG

更新日期：2002-12-04 00:00:00

abstract：:Southern blotting using a 5'-proximal probe of the Saccharomyces cerevisiae CYR1 gene has revealed heterogeneity in laboratory strains. It is demonstrated that strain AB320 contains a Ty1 element inserted in the promoter region of CYR1. The Ty1 orientation suggests that transcription of CYR1 is initiated downstream fr...

journal_title：FEBS letters

authors： Lenzen G,Masson P,Jacquemin JM,Danchin A

更新日期：1987-07-13 00:00:00

abstract：:Laser flash-induced photovoltage responses of the D85S and D85T mutants as well as of the wild-type acid blue form are similar and reflect intraprotein charge redistribution caused by retinal isomerization. The Cl- -induced transition of all of these blue forms into purple ones is accompanied by the appearance of elec...

journal_title：FEBS letters

authors： Kalaidzidis IV,Kaulen AD

更新日期：1997-12-01 00:00:00

abstract：:Neurons grow during development and extend long axons to make contact with their targets with the help of an intrinsic program of axonal growth as well as a range of extrinsic cues and a permissive milieu. Injury events in adulthood induce some neuron types to revert to a regenerative state in the peripheral nervous s...

journal_title：FEBS letters

authors： Doron-Mandel E,Fainzilber M,Terenzio M

更新日期：2015-06-22 00:00:00

abstract：:The binding of glycosaminoglycans to a synthetic peptide (SKAQKAQAKQAKQAQKAQKAQAKQAKQW-CONH(2)), consisting of a hybrid consensus heparin binding sequence, is studied using circular dichroism, fluorescence anisotropy and nuclear magnetic resonance techniques. The results unveil certain novel features, most importantly...

journal_title：FEBS letters

authors： Jayaraman G,Wu CW,Liu YJ,Chien KY,Fang JC,Lyu PC

更新日期：2000-09-29 00:00:00

abstract：:We show that inverted membrane vesicles from Corynebacterium glutamicum, a Gram-positive bacterium, are able to generate and maintain an electrochemical gradient of protons in response to the addition of NADH. This result indicates that the respiratory chain is intact and that the vesicles are reasonably impermeable t...

journal_title：FEBS letters

authors： Schrempp S,Bayan N,Shechter E

更新日期：1994-12-12 00:00:00

abstract：:Gastric H+,K+-ATPase activity is not affected by Na+ at pH 7.0 but is significantly stimulated by Na+ at pH 8.5. For the stimulation at the latter pH, the presence of both Na+ and K+ were essential. Contrary the H+,K+-ATPase, the associated K+-pNPPase was inhibited by Na+ at both pH values. Sodium competes with K+ for...

journal_title：FEBS letters

authors： Ray TK,Nandi J

更新日期：1985-06-03 00:00:00

abstract：:The role of p53 as the "guardian of the genome" in differentiated somatic cells, triggering various biological processes, is well established. Recent studies in the stem cell field have highlighted a profound role of p53 in stem cell biology as well. These studies, combined with basic data obtained 20 years ago, provi...

journal_title：FEBS letters

authors： Aloni-Grinstein R,Shetzer Y,Kaufman T,Rotter V

更新日期：2014-08-19 00:00:00

abstract：:In a previous investigation of the rDNA region in Tetrahymena pyriformis mitochondrial DNA, we identified a putative tRNA(Met) gene [Heinonen et al. (1987) J. Biol. Chem. 262, 2879-2887]. On the basis of Northern hybridization analyses, we suggested that this gene is expressed, even though the resulting tRNA would be ...

journal_title：FEBS letters

authors： Schnare MN,Greenwood SJ,Gray MW

更新日期：1995-03-27 00:00:00

abstract：:In kidney cortex tubules isolated from fed rabbits L-alanine is not utilized as glucose precursor, when added as a sole substrate. However, this amino acid decreases gluconeogenesis from low (up to 1 mM) 2-oxoglutarate concentrations and stimulates this process at higher (2.5-10 mM) ketoacid contents in the suspension...

journal_title：FEBS letters

authors： Zabłocki K,Bryła J

更新日期：1989-12-18 00:00:00