Abstract:
:Many receptors coupled to inhibitory Go/Gi-type G proteins often also produce stimulatory signals like Ca2+ mobilisation. When expressed in CHO cells the alpha2-adrenoceptor subtypes alpha2A, alpha2B and alpkha2C mobilised Ca2+. These responses were strongly reduced by the P2Y-purinoceptor antagonist suramin. A large proportion of the total pool of purine nucleotides was found extracellularly. Removal of extracellular nucleotides with apyrase or by constant perfusion had a similar effect as suramin. These treatments did not affect the alpha2-adrenoceptor-mediated inhibition of cAMP production. This indicates that cells may be primed or their signaling pathways redirected towards Ca2+ mobilisation by 'autocrine' release of nucleotides.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Akerman KE,Näsman J,Lund PE,Shariatmadari R,Kukkonen JPdoi
10.1016/s0014-5793(98)00664-4subject
Has Abstractpub_date
1998-07-03 00:00:00pages
209-12issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00664-4journal_volume
430pub_type
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