Exploration of the spontaneous fluctuating activity of single enzyme molecules.

abstract：:Resonance Raman (RR) spectra of the monomeric cyanomethaemoglobin CTT III from insect larvae of Chironomus thummi thummi are shown for the range of 200-550 cm-1. By iron and cyanide isotope exchange a line varying between 307 cm-1 for 57Fe-13C15N and 311 cm-1 for 54Fe-12C14N, has been assigned to the Fe-N epsilon stre...

journal_title：FEBS letters

authors： Kerr EA,Yu NT,Gersonde K

更新日期：1984-12-03 00:00:00

abstract：:Rat brain microtubules were prepared at the adult stage and from immature (i.e., 4-day-old) animals. At an early stage of development, the composition of microtubule-associated proteins is qualitatively different from that found at the adult stage [(1982) Eur. J. Biochem. 129, 465-471]. The influence of calmodulin on ...

journal_title：FEBS letters

authors： Erneux C,Passareiro H,Nunez J

更新日期：1984-07-09 00:00:00

abstract：:Saccharomyces cerevisiae mutant strain DTY26, lacking ACE1, the protein mediator for the induction of metallothionein gene expression, is unable to increase Cu,Zn superoxide dismutase mRNA in response to copper. In the wild-type strain DTY22 transcription of both Cu,Zn superoxide dismutase and metallothionein genes is...

journal_title：FEBS letters

authors： Carri MT,Galiazzo F,Ciriolo MR,Rotilio G

更新日期：1991-01-28 00:00:00

abstract：:The relevance of MDR-1 gene expression to the multidrug resistance phenotype was investigated. Drug-resistant cells, KB-V1 and MCF7/ADR, constantly expressed mRNA of the MDR-1 gene and were more resistant to vinblastine and adriamycin than drug-sensitive cells, KB-3-1 and MCF7. The drug efflux rate of KB-V1 was the sa...

journal_title：FEBS letters

authors： Kim HM,Oh GT,Hong DH,Kim MS,Kang JS,Park SM,Han SB

更新日期：1997-07-21 00:00:00

abstract：:The effect of the myotoxic drug chlorpromazine was studied in vitro on proteins of sarcoplasmic reticulum and mitochondrial matrix of skeletal muscle in the normal mouse. Our results indicate that the drug is specific for calcium-binding proteins (calcium ATPase, calsequestrin and calmitine). Its proteolytic effect on...

journal_title：FEBS letters

authors： Lucas-Heron B,Le Ray B,Schmitt N

更新日期：1995-11-06 00:00:00

abstract：:Two major embryonic myosin heavy chains are expressed in embryonic chick breast muscle until the first week after hatching. Of these, one is already detected in the 8-day-old embryo. The other appears on day 12. Two putative slow embryonic isoforms represent minor components transiently expressed between days 8 and 12...

journal_title：FEBS letters

authors： Hofmann S,Düsterhöft S,Pette D

更新日期：1988-10-10 00:00:00

abstract：:Reconstitution of the 16 kDa N-terminal domain of the peridinin-chlorophyll-protein, N-PCP, with mixtures of chlorophyll a (Chl a) and Chl b, resulted in 32 kDa complexes containing two pigment clusters, each bound to one N-PCP. Besides homo-chlorophyllous complexes, hetero-chlorophyllous ones were obtained that conta...

journal_title：FEBS letters

authors： Brotosudarmo TH,Hofmann E,Hiller RG,Wörmke S,Mackowski S,Zumbusch A,Bräuchle C,Scheer H

更新日期：2006-10-02 00:00:00

abstract：:Treatment of human neutrophils with triphenyltin chloride (TPTCl)-inhibited superoxide (O-2) production stimulated with phorbol myristate acetate (PMA). TPTCl was more potent as inhibitor of O-2 production than other phenyltin compounds. The O-2 production by the xanthine oxidase-acetaldehyde system was not inhibited ...

journal_title：FEBS letters

authors： Matsui H,Wada O,Ushijima Y,Akuzawa T

更新日期：1983-12-12 00:00:00

abstract：:We investigated in vitro effects of recombinant human thrombopoietin (TPO), or c-Mpl ligand, on human platelets. TPO induced rapid dose-dependent tyrosine phosphorylation of several proteins. We identified Janus tyrosine kinases, Tyk2 and JAK2, and a member of STAT (signal transducers and activators of transcription) ...

journal_title：FEBS letters

authors： Ezumi Y,Takayama H,Okuma M

更新日期：1995-10-23 00:00:00

abstract：:Flagellins of two Escherichia coli strains have been investigated by limited proteolysis and scanning microcalorimetry. It has been shown that a monomer flagellin consists of two parts: a central one cooperatively melting, rather resistant to proteases, and the other without a stable tertiary structure and thus easily...

journal_title：FEBS letters

authors： Kostyukova AS,Pyatibratov MG,Filimonov VV,Fedorov OV

更新日期：1988-12-05 00:00:00

abstract：:The surprising result that the spanning domain causes retention of proteins in the Golgi stack poses the question as to the actual mechanism. Here we present a simple model that might have general applicability. ...

journal_title：FEBS letters

authors： Nilsson T,Slusarewicz P,Hoe MH,Warren G

更新日期：1993-09-06 00:00:00

abstract：:Glucose-regulated protein 58 (GRP58)-like immunoreactivity in rat liver obtained in the evening or after fasting underwent an electrophoretic band-shift, which disappeared after phosphatase-treatment. Since mass spectrometric analysis raised a possibility that Ser150 of GRP58 is phosphorylated, an antibody against the...

journal_title：FEBS letters

authors： Kita K,Okumura N,Takao T,Watanabe M,Matsubara T,Nishimura O,Nagai K

更新日期：2006-01-09 00:00:00

abstract：:In Ruminococcus albus, 4-O-β-D-mannosyl-D-glucose phosphorylase (RaMP1) and β-(1,4)-mannooligosaccharide phosphorylase (RaMP2) belong to two subfamilies of glycoside hydrolase family 130. The two enzymes phosphorolyze β-mannosidic linkages at the nonreducing ends of their substrates, and have substantially diverse sub...

journal_title：FEBS letters

authors： Ye Y,Saburi W,Odaka R,Kato K,Sakurai N,Komoda K,Nishimoto M,Kitaoka M,Mori H,Yao M

更新日期：2016-03-01 00:00:00

abstract：:In dog-fish spermatid nuclei two intermediate proteins S1 and S2 replace histones before the setting down of protamines. These spermatid-specific proteins were isolated by carboxymethyl-cellulose chromatography and purified by high pressure liquid chromatography. S1 and S2 are characterized by a high content of basic ...

journal_title：FEBS letters

authors： Chauviere M,Laine B,Sautiere P,Chevaillier P

更新日期：1983-02-21 00:00:00

abstract：:Antipsychotic drugs of known antidiarrhoeal and anticalmodulin activity inhibited the cholera-toxin-catalysed ADP-ribosylation of proteins of Mr 37,000, 40,000 and 45,000 (thought to be regulatory components of the adenylate cyclase complex) that was previously shown to occur in plasma membranes from rabbit intestinal...

journal_title：FEBS letters

authors： Longbottom D,van Heyningen S

更新日期：1990-10-15 00:00:00

abstract：:In C4 plants the activity of phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) is regulated by phosphorylation/dephosphorylation which is mediated by light/dark signals. The study using protein kinase inhibitors showed that the inhibition pattern of maize PEPC-protein kinase (PEPC-PK) is similar to that of myosin li...

journal_title：FEBS letters

authors： Ogawa N,Okumura S,Izui K

更新日期：1992-05-04 00:00:00

abstract：:Bovine insulin can be split into several fractions by gel filtration on Sephadex G50. These are, in order of decreasing molecular weight, a new unidentified substance, proinsulin, and a large fraction containing several substances. Ultracentrifugal examination of the proinsulin in acetic acid solution (pH 2.3) gave a ...

journal_title：FEBS letters

authors： Zühlke H,Behlke J

更新日期：1968-12-01 00:00:00

abstract：:Light stimulation of rod cells in vertebrate eyes may cause Ca2+ release from the intracellular disks. Radiolabelled tracers show that light causes a small hyperpolarization of intact disk stacks and redistribution of the ions Ca2+ and Cl-. ...

journal_title：FEBS letters

authors： Hughes SM,Brand MD

更新日期：1985-03-25 00:00:00

abstract：:Most tumor cells attain their immortality by reactivating telomerase. We report here the telomerase inhibitory potential of chimeric oligonucleotides composed of a 13mer antisense sequence targeting the telomerase RNA template region and a (s4dU)n moiety at its 3' or 5'-end. The increase of the thiolated chain length ...

journal_title：FEBS letters

authors： Tarkanyi I,Horváth A,Szatmari I,Eizert H,Vámosi G,Damjanovich S,Ségal-Bendirdjian E,Aradi J

更新日期：2005-02-28 00:00:00

abstract：:The amino acid sequence and part of the DNA sequence of a glutamic acid-specific serine protease from Streptomyces griseus is reported. This protease is shown to be homologous with other serine proteases. An improved purification protocol for this enzyme is described. ...

journal_title：FEBS letters

authors： Svendsen I,Jensen MR,Breddam K

更新日期：1991-11-04 00:00:00

abstract：:DNA-dependent RNA polymerase from blood forms and culture forms of the parasitic protozoan Trypanosoma brucei was resolved into multiple peaks of activity by DEAE-Sephadex chromatography. The enzyme from the two forms was found to be different by several criteria, suggesting that it is subject to developmental control...

journal_title：FEBS letters

authors： Tittawella I

更新日期：1988-01-25 00:00:00

abstract：:It has been suggested that the C-terminal domain of Bcl-2 family members may contain a signal anchor sequence that targets these proteins to the mitochondrial outer membrane. We have investigated the consequence of deleting this domain upon cytochrome c release in yeast strains that coexpress truncated forms of Bax (i...

journal_title：FEBS letters

authors： Priault M,Camougrand N,Chaudhuri B,Manon S

更新日期：1999-01-25 00:00:00

abstract：:A number of cDNA clones in summary encoding 700 amino acid residues from the N-end of rat liver elongation factor 2 (EF-2) and including 49 nucleotides of the 5'-untranslated mRNA region have been obtained. EF-2 cDNA clones were isolated from gradually constructed small (1000-5000 clones) specific cDNA libraries using...

journal_title：FEBS letters

authors： Oleinikov AV,Jokhadze GG,Alakhov YuB

更新日期：1989-05-08 00:00:00

abstract：:Friedreich ataxia is a recessively inherited neurodegenerative disease caused by deficiency of a highly conserved mitochondrial protein, frataxin. Frataxin deficiency results in mitochondrial iron accumulation and oxidative stress. Frataxin shows homology with the CyaY proteins of gamma-purple bacteria, whose function...

journal_title：FEBS letters

authors： Li DS,Ohshima K,Jiralerspong S,Bojanowski MW,Pandolfo M

更新日期：1999-07-30 00:00:00

abstract：:Glycogen phosphorylase b at concentrations close to those found in skeletal muscle interacts with sarcoplasmic reticulum membranes, but not with liposomes made of lipids extracted from these membranes, and is inhibited upon binding to the membrane. The interaction of glycogen phosphorylase with the sarcoplasmic reticu...

journal_title：FEBS letters

authors： Cuenda A,Centeno F,Gutierrez-Merino C

更新日期：1991-06-03 00:00:00

abstract：:The two subunits of gamma-glutamyl transpeptidase (EC 2.3.2.2) are derived from a single-chain glycosylated precursor. A small fraction of the propeptide survives proteolytic processing in the rat kidney and has been purified by an immunoaffinity technique. The propeptide contains determinants for both the subunits an...

journal_title：FEBS letters

authors： Tate SS

更新日期：1986-01-01 00:00:00

abstract：:We applied 1D homonuclear Hartmann-Hahn (1D-HOHAHA) and difference NOE experiments to determine the chemical structure of Forssman's antigen, a glycolipid purified from sheep red blood cells. The subspectra corresponding to the individual sugar components were extracted from overlapping proton resonances by selective ...

journal_title：FEBS letters

authors： Inagaki F,Kodama C,Suzuki M,Suzuki A

更新日期：1987-07-13 00:00:00

abstract：:The TOE1 gene was discovered as a target of the Egr1 transcription factor that participates in cell growth regulation through the upregulation of p21 and a cell cycle delay at the G2/M phase. We report here that TOE1 is able to bind to the p53 tumor suppressor protein, specifically interacting with the C terminal tetr...

journal_title：FEBS letters

authors： Sperandio S,Tardito S,Surzycki A,Latterich M,de Belle I

更新日期：2009-07-07 00:00:00

abstract：:Hemocyanin is present as 2 subunits in the hemolymph of Penaeus vannamei. Isolated from a hepatopancreas cDNA library of this penaeid shrimp, the cDNA chain (2095 bp) corresponds to a full length hemocyanin messenger as determined by Northern hybridization, with a short 5' untranslated region (17 bp), an open reading ...

journal_title：FEBS letters

authors： Sellos D,Lemoine S,Van Wormhoudt A

更新日期：1997-04-28 00:00:00

abstract：:The human gene frataxin and its yeast homolog YFH1 affect mitochondrial function. Deficits in frataxin result in Friedreich ataxia, while deletion of YFH1 results in respiratory incompetence. We determined that as long as respiratory incompetent yeast express Yfh1p they do not accumulate excessive mitochondrial iron. ...

journal_title：FEBS letters

authors： Chen OS,Kaplan J

更新日期：2001-11-30 00:00:00