Histidine-419 plays a role in energy coupling in the vesicular monoamine transporter from rat.

abstract：:Screening of hemorphins in extracts of rat lung, brain, heart and spleen was carried out. The threshold for detection of hemorphins was 0.01 nmol for spleen and 0.05 nmol for other tissues. Both the content and the composition of hemorphins differed significantly in the tissues analyzed. Heart and lung extracts were r...

journal_title：FEBS letters

authors： Yatskin ON,Philippova MM,Blishchenko EYu,Karelin AA,Ivanov VT

更新日期：1998-05-29 00:00:00

abstract：:A reversible two-step (native state<-->denatured state) folding mechanism based on equilibrium and stopped flow experiments is proposed for urea denaturation of the lipoyl-bearing domain (hbLBD) of human mitochondrial branched chain alpha-ketoacid dehydrogenase (BCKD) complex. The results from this circular dichroism ...

journal_title：FEBS letters

authors： Naik MT,Chang YC,Huang TH

更新日期：2002-10-23 00:00:00

abstract：:Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1beta 10(-10) M, tumour necrosis factor-alpha 10(-10) M, interferon-gamma 5 U/ml) led to extensive cell death, which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-treatment with insulin (25 ng/ml) or i...

journal_title：FEBS letters

authors： Harrison M,Dunger AM,Berg S,Mabley J,John N,Green MH,Green IC

更新日期：1998-09-18 00:00:00

abstract：:T cells are stimulated by the engagement of antigen, cytokine, pathogen, and hormone receptors. While research performed over many years has focused on deciphering the molecular components of these pathways, recent data underscore the importance of the metabolic environment in conditioning responses to receptor engage...

journal_title：FEBS letters

authors： Yong CS,Abba Moussa D,Cretenet G,Kinet S,Dardalhon V,Taylor N

更新日期：2017-10-01 00:00:00

abstract：:PsbV2 is a c-type cytochrome present in a very low abundance in the thermophilic cyanobacterium Thermosynechococcus elongatus. We purified this cytochrome and solved its crystal structure at a resolution of 1.5Å. The protein existed as a dimer in the crystal, and has an overall structure similar to other c-type cytoch...

journal_title：FEBS letters

authors： Suga M,Lai TL,Sugiura M,Shen JR,Boussac A

更新日期：2013-10-01 00:00:00

abstract：:Jeotgalicoccus sp. 8456 OleTJE (CYP152L1) is a fatty acid decarboxylase cytochrome P450 that uses hydrogen peroxide (H2 O2 ) to catalyse production of terminal alkenes, which are industrially important chemicals with biofuel applications. We report enzyme fusion systems in which Streptomyces coelicolor alditol oxidase...

journal_title：FEBS letters

authors： Matthews S,Tee KL,Rattray NJ,McLean KJ,Leys D,Parker DA,Blankley RT,Munro AW

更新日期：2017-03-01 00:00:00

abstract：:Cellular retinoic acid binding proteins (CRABPs) bind all-trans-retinoic acid (atRA) tightly. This study aimed to determine whether atRA is channeled directly to cytochrome P450 (CYP) CYP26B1 by CRABPs, and whether CRABPs interact directly with CYP26B1. atRA bound to CRABPs (holo-CRABP) was efficiently metabolized by ...

journal_title：FEBS letters

authors： Nelson CH,Peng CC,Lutz JD,Yeung CK,Zelter A,Isoherranen N

更新日期：2016-08-01 00:00:00

abstract：:Protein misfolding and aggregation are linked to several degenerative diseases and are responsible for the formation of bacterial inclusion bodies. Roles of molecular chaperones in promoting protein deposition have been speculated but not proven in vivo. We have investigated the involvement of individual chaperones in...

journal_title：FEBS letters

authors： Carrió MM,Villaverde A

更新日期：2003-02-27 00:00:00

abstract：:Guanylate cyclase activity was measured in disrupted rod outer segments of the toad retina. The experiments showed that cGMP is synthesized from GTP at a rate of 3 +/- 1 nmol/min per mg protein. In darkness this value is largely independent of the Ca2+ concentration, while it is enhanced by flashes of light of increas...

journal_title：FEBS letters

authors： Pepe IM,Panfoli I,Cugnoli C

更新日期：1986-07-14 00:00:00

abstract：:Gastric H+,K+-ATPase activity is not affected by Na+ at pH 7.0 but is significantly stimulated by Na+ at pH 8.5. For the stimulation at the latter pH, the presence of both Na+ and K+ were essential. Contrary the H+,K+-ATPase, the associated K+-pNPPase was inhibited by Na+ at both pH values. Sodium competes with K+ for...

journal_title：FEBS letters

authors： Ray TK,Nandi J

更新日期：1985-06-03 00:00:00

abstract：:Incubation of rat skeletal muscle mitochondria with the nitric oxide generator, S-nitrosoglutathione (GSNO) reversibly inhibited oxygen utilisation with all substrates tested. The visible absorption spectra of the inhibited mitochondria showed that cytochromes c+c1, b and a+a3 were reduced, indicating a block at the d...

journal_title：FEBS letters

authors： Cleeter MW,Cooper JM,Darley-Usmar VM,Moncada S,Schapira AH

更新日期：1994-05-23 00:00:00

abstract：:We present here a combined GC-MS and LC-MS metabolic profiling approach to unraveling the pathological outcomes of aristolochic acid (AA)-induced nephrotoxicity. Urine samples were analyzed by GC-MS and LC-MS in combination with pattern recognition techniques, e.g. principal component analysis (PCA), orthogonal projec...

journal_title：FEBS letters

authors： Ni Y,Su M,Qiu Y,Chen M,Liu Y,Zhao A,Jia W

更新日期：2007-02-20 00:00:00

abstract：:Nitric oxide (.NO) synthase (NOS) activity in subcellular fractions from cultured endothelial cells (EC) and lipopolysaccharide-activated J774.2 monocyte/macrophages was investigated by monitoring the .NO-mediated increase in intracellular cyclic GMP in LLC-PK1 pig kidney epithelial cells. The constitutive NOS in EC (...

journal_title：FEBS letters

authors： Hecker M,Walsh DT,Vane JR

更新日期：1991-12-09 00:00:00

abstract：:Preillumination of intact cells of the eukaryotic, halotolerant, cell-wall-less green alga Dunaliella salina induces a dark ATPase activity the magnitude of which is about 3-5-fold higher than the ATPase activity observed in dark-adapted cells. The light-induced activity arises from the activation and stabilization in...

journal_title：FEBS letters

authors： Selman-Reimer S,Selman BR

更新日期：1988-03-28 00:00:00

abstract：:Oil crops are in increasing demand both for food and as renewable sources of chemicals. It is therefore vital to understand how oil accumulation is regulated. Different ways of obtaining such information are discussed with an emphasis on metabolic control analysis. The usefulness of the latter has been well-illustrate...

journal_title：FEBS letters

authors： Harwood JL,Guschina IA

更新日期：2013-06-27 00:00:00

abstract：:Rapidly generated high-titer Semliki Forest virus (SFV) vectors can infect numerous mammalian cell lines and primary cell cultures, and result in high levels of transgene expression. SFV-based expression of transmembrane receptors has been characterized by specific ligand-binding activity and functional responses. Ada...

journal_title：FEBS letters

authors： Lundstrom K,Schweitzer C,Rotmann D,Hermann D,Schneider EM,Ehrengruber MU

更新日期：2001-08-31 00:00:00

abstract：:Drug carriers such as liposomes are not readily transported across cellular barriers that constitute epithelia. However, certain epithelia (rabbit yolk sac endoderm and enterocytes of suckling rat gut proximal small intestine) are well known to transcytose maternal IgG by Fc receptor-mediated endocytic events. We have...

journal_title：FEBS letters

authors： Patel HM,Wild AE

更新日期：1988-07-18 00:00:00

abstract：:Treatment of HepG2 with all-trans retinoic acid (RA) induces expression of fatty acid synthase (FAS) mRNA and protein. Transfections show that the FAS promoter positively responds to retinoid X receptor (RXR) but not to RA receptor (RAR) agonists. Since RXR alone is capable of mediating the RA response of FAS, the exi...

journal_title：FEBS letters

authors： Roder K,Zhang L,Schweizer M

更新日期：2007-06-12 00:00:00

abstract：:A lowered efficiency of oxidative phosphorylation was recently found in a Leber hereditary optic neuropathy (LHON) proband carrying a mutation in the mtDNA gene for subunit 6 of the membrane-bound F0 segment of the F1F0-ATP synthase [9]. This phenotype was transferred to cytoplasmic hybrid cells together with the muta...

journal_title：FEBS letters

authors： Majander A,Lamminen T,Juvonen V,Aula P,Nikoskelainen E,Savontaus ML,Wikström M

更新日期：1997-07-28 00:00:00

abstract：:A monoclonal antibody, MANDYS141, binds to both dystrophin and actinin on Western blots (SDS-denatured), but only to actinin in frozen sections of human muscle (native conformation). It differs from a polyclonal cross-reacting antiserum in that it binds to several muscle isoforms of actinin (smooth, fast and slow) fro...

journal_title：FEBS letters

authors： Nguyen TM,Ellis JM,Ginjaar IB,van Paassen MM,van Ommen GJ,Moorman AF,Cartwright AJ,Morris GE

更新日期：1990-10-15 00:00:00

abstract：:The oxidoreductase ERp57 is a component of the major histocompatibility complex (MHC) class I peptide-loading complex. ERp57 can interact directly with MHC class I molecules, however, little is known about which of the cysteine residues within the MHC class I molecule are relevant to this interaction. MHC class I mole...

journal_title：FEBS letters

authors： Antoniou AN,Santos SG,Campbell EC,Lynch S,Arosa FA,Powis SJ

更新日期：2007-05-15 00:00:00

abstract：:Trace amounts of gangliosides were found in rabbit skeletal muscle sarcoplasmic reticulum and their main part was shown, by high performance thin layer chromatography. to be GM3. Addition of GM3 to the soybean phospholipids used for reconstitution of proteoliposomes markedly increased ATP hydrolysis as well as Ca2+ up...

journal_title：FEBS letters

authors： Wang LH,Tu YP,Yang XY,Tsui ZC,Yang FY

更新日期：1996-06-17 00:00:00

abstract：:Synthetic high- and low-molecular-mass atrial peptides were phosphorylated in vitro by cyclic AMP-dependent protein kinase and [32P]ATP. From a series of atrial peptide analogs, it was deduced that the amino acid sequence, Arg101-Ser104 of atriopeptin was required for optimal phosphorylation. Phosphorylated AP(99-126)...

journal_title：FEBS letters

authors： Olins GM,Mehta PP,Blehm DJ,Patton DR,Zupec ME,Whipple DE,Tjoeng FS,Adams SP,Olins PO,Gierse JK

更新日期：1987-11-30 00:00:00

abstract：:Heme is essential and synthesized via highly regulated processes. For this reason, most organisms strive to recycle it or acquire it from their environment. When heme is bound to proteins noncovalently, degradation of the polypeptide is sufficient to release it. However, in some hemoproteins, such as c-type cytochrome...

journal_title：FEBS letters

authors： Murphey AC,Mavridou DAI,Hodgkin J,Ferguson SJ

更新日期：2018-03-01 00:00:00

abstract：:Cerebellar granule cells differentiated in culture were incubated with ganglioside [3H-Sph]GM1 in order to have it inserted into the plasma membrane and metabolized. Among the formed metabolites radioactive sphingosine and ceramide were identified. [3H]Ceramide started to be measurable after 10 min of incubation (puls...

journal_title：FEBS letters

authors： Riboni L,Bassi R,Sonnino S,Tettamanti G

更新日期：1992-03-30 00:00:00

abstract：:The possible regulation of the expression of uncoupling protein-2 (UCP2) mRNA by thyroid hormones in different tissues was examined in rats. Triiodothyronine (T3) was found to produce an organ-specific enhancement of UCP2 expression in rat tissues. The effect of T3 was markedly observed in heart, whereas a moderate ef...

journal_title：FEBS letters

authors： Lanni A,De Felice M,Lombardi A,Moreno M,Fleury C,Ricquier D,Goglia F

更新日期：1997-11-24 00:00:00

abstract：:The cofactor of several flavoenzymes is autocatalytically bound to the polypeptide via a histidyl(N3)-(8alpha)-FAD linkage which makes the generation of apoenzyme difficult. We introduced an alternative covalent protein-FAD bond at the active site of 6-hydroxy-D-nicotine oxidase (6HDNO) by replacing the FAD-binding hi...

journal_title：FEBS letters

authors： Stoltz M,Henninger HP,Brandsch R

更新日期：1996-05-20 00:00:00

abstract：:The red cell membrane is comprised of a lipid bilayer studded with transmembrane proteins, and laminated by a protein network, the membrane skeleton, at the surface of the inner monolayer. The erythrocyte owes its mechanical properties to the membrane skeleton. Hereditary spherocytosis, hereditary elliptocytosis or po...

journal_title：FEBS letters

authors： Delaunay J

更新日期：1995-08-01 00:00:00

abstract：:Incubation of calf thymus DNA with 3-morpholinosydnonimine (SIN-1), which simultaneously generates nitric oxide (NO) and superoxide (O2-), induced a significant increase of 8-hydroxydeoxyguanosine (8-OH-dG). Peroxynitrite also increased 8-OH-dG in calf thymus DNA. Addition of free hydroxyl radical (.OH) scavengers inh...

journal_title：FEBS letters

authors： Inoue S,Kawanishi S

更新日期：1995-08-28 00:00:00

abstract：:The verprolin family of proteins, WIP, CR16 and WIRE/WICH, has emerged as critical regulators of cytoskeletal organisation in vertebrate cells. The founding father of the family, verprolin, was originally identified in budding yeast and later shown to be needed for actin polymerisation during polarised growth and duri...

journal_title：FEBS letters

authors： Aspenström P

更新日期：2005-10-10 00:00:00