Guanylate cyclase in rod outer segments of the toad retina. Effect of light and Ca2+.

abstract：:USP4, 11 and 15 are three closely related paralogues of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. The DUSP domain and the UBL domain in these proteins are juxtaposed which may provide a functional unit conferring specificity. We determined the structures of the USP15 DUSP-UBL double dom...

journal_title：FEBS letters

authors： Elliott PR,Liu H,Pastok MW,Grossmann GJ,Rigden DJ,Clague MJ,Urbé S,Barsukov IL

更新日期：2011-11-04 00:00:00

abstract：:Sensitivity of mammalian cells to the bacterial toxin aerolysin is due to the presence at their surface of glycosylphosphatidyl inositol (GPI)-anchored proteins which act as receptors. Using a panel of mutants that are affected in the GPI biosynthetic pathway and Trypanosoma brucei variant surface glycoproteins, we sh...

journal_title：FEBS letters

authors： Abrami L,Velluz MC,Hong Y,Ohishi K,Mehlert A,Ferguson M,Kinoshita T,Gisou van der Goot F

更新日期：2002-02-13 00:00:00

abstract：:Insulin causes a translocation of glucose transporters from intracellular microsomes to the plasma membrane in adipocytes. To determine whether insulin has a similar effect in rat hindlimb muscles, we used glucose-inhibitable cytochalasin B binding to estimate the number of glucose transporters in membrane fractions f...

journal_title：FEBS letters

authors： Klip A,Ramlal T,Young DA,Holloszy JO

更新日期：1987-11-16 00:00:00

abstract：:Gemin5 acts as a U1 small nuclear RNA (snRNA)-binding protein in U1 small nuclear ribonucleic protein (snRNP) biogenesis. Here, we report a role for Gemin5 in unassembled U1 snRNP disposal under survival of motor neuron (SMN) protein-deficient conditions. We demonstrate that non-Sm protein-associated U1 snRNA and U1A ...

journal_title：FEBS letters

authors： Jiang D,Zou X,Zhang C,Chen J,Li Z,Wang Y,Deng Z,Wang L,Chen S

更新日期：2018-04-01 00:00:00

abstract：:We have demonstrated over a decade ago that hepoxilins cause the release of insulin from isolated pancreatic islets of Langerhans in vitro. However, no studies are available so far to indicate whether these compounds are active in vivo. The present study is the first to our knowledge which demonstrates that hepoxilins...

journal_title：FEBS letters

authors： Pace-Asciak CR,Demin PM,Estrada M,Liu G

更新日期：1999-11-19 00:00:00

abstract：:Several studies have indicated the existence of direct effects of melatonin on platelets. Here we show that, melatonin at high concentration is capable of significantly raising platelet intracellular calcium even in the absence of an agonist. The effect of melatonin on platelets was abolished by luzindole, a melatonin...

journal_title：FEBS letters

authors： Kumari S,Dash D

更新日期：2011-07-21 00:00:00

abstract：:We examined whether a proposed spatial proximity between Asp114(2.50) and Asn332(7.49) affected the functional properties of the mu opioid receptor. The D114(2.50)N mutant had reduced binding affinities for morphine, DAMGO and CTAP, but not for naloxone and [3H]diprenorphine; this mutation also abolished agonist-induc...

journal_title：FEBS letters

authors： Xu W,Ozdener F,Li JG,Chen C,de Riel JK,Weinstein H,Liu-Chen LY

更新日期：1999-03-26 00:00:00

abstract：:Amino acid sequences coded for by 13 different genes of mammalian mitochondrial DNA (mtDNA) including 8 unassigned open reading frames (URFs) were compared in pairs. It was found that significant homologies exist among the amino acid sequences of the three URFs (URF2, URF4 and URF5) with a probability of occurrence of...

journal_title：FEBS letters

authors： Kikuno R,Miyata T

更新日期：1985-09-09 00:00:00

abstract：:To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCPI) in white fat, mitochondrial membrane potential (delta psi(m)) was estimated by flow cytometry in adipocytes isolated from gonadal fat. Ectopic UCP1...

journal_title：FEBS letters

authors： Baumruk F,Flachs P,Horáková M,Floryk D,Kopecký J

更新日期：1999-02-12 00:00:00

abstract：:Dark-adapted isolated spinach chloroplasts and leaves, unlike sub-chloroplast fractions, are capable of emitting ultraweak light spontaneously (50-125 counts/s per cm2). The emission of leaves is due to two processes with activation energies of 97 and 25 kJ/mol while in isolated chloroplasts, it is the result of a sin...

journal_title：FEBS letters

authors： Hideg E,Kobayashi M,Inaba H

更新日期：1990-11-26 00:00:00

abstract：:The vasopressin gene is highly transcribed in magnocellular neurons of the supraoptic (SON) and paraventricular nucleus (PVN) in the rat hypothalamus. In order to identify cis-acting elements involved in the expression of the vasopressin gene, approximately 1 kb upstream of the transcription start site has been sequen...

journal_title：FEBS letters

authors： Mohr E,Richter D

更新日期：1990-01-29 00:00:00

abstract：:An up to 14-fold increase in total hexokinase activity induced by low-frequency stimulation in rat fast-twitch muscle was followed by a rapid decay in enzyme activity after cessation of stimulation. In vivo labeling revealed that these alterations were related to rapid changes in [35S]methionine incorporation into hex...

journal_title：FEBS letters

authors： Weber FE,Pette D

更新日期：1990-02-26 00:00:00

abstract：:The small GTPase Rho1 plays an essential role in controlling the organization of the actin cytoskeleton and synthesis of the cell wall in the fission yeast Schizosaccharomyces pombe. Here we studied the role of Rho5 whose primary structure is very similar to that of Rho1. It was found that elevated expression of Rho5 ...

journal_title：FEBS letters

authors： Nakano K,Arai R,Mabuchi I

更新日期：2005-09-26 00:00:00

abstract：:Cytosolic Ca2+ concentration and membrane potential were monitored in individual cultured endothelial cells mechanically stimulated with a micropipette attached to the stage of a microscope. Both dimpling and poking of endothelial cells resulted in Ca2+i transients (from 63 +/- 12 to 397 +/- 52 nM, characterized by a ...

journal_title：FEBS letters

authors： Goligorsky MS

更新日期：1988-11-21 00:00:00

abstract：:The fibroblast growth factor receptor substrate (FRS)-2 protein family comprises FRS2alpha, a well-known central mediator for fibroblast growth factor signaling, and FRS2beta, whose endogenous expression pattern and function are not yet defined. Immunohistochemical analysis revealed that expression of FRS2beta was res...

journal_title：FEBS letters

authors： Minegishi Y,Iwanari H,Mochizuki Y,Horii T,Hoshino T,Kodama T,Hamakubo T,Gotoh N

更新日期：2009-02-18 00:00:00

abstract：:The X-ray structure of bovine beta-lactoglobulin with the ligand 12-bromododecanoic acid as a model for fatty acids has been determined at a resolution of 2.23 A in the trigonal lattice Z form. The ligand binds inside the calyx, resolving a long-standing controversy as to where fatty-acid like ligands bind. The carbox...

journal_title：FEBS letters

authors： Qin BY,Creamer LK,Baker EN,Jameson GB

更新日期：1998-11-06 00:00:00

abstract：:The amino acid sequence of the alpha-subunit of Gi, the human adenylate cyclase inhibiting GTP-binding protein, has been deduced from the nucleotide sequence of a DNA clone complementary to Gi alpha mRNA from differentiated U937 cells. The cDNA encodes a polypeptide of 355 amino acids (Mr 40456). The amino acid sequen...

journal_title：FEBS letters

authors： Didsbury JR,Ho YS,Snyderman R

更新日期：1987-01-26 00:00:00

abstract：:A procedure for isolation of two iso-plastocyanins from parsley has been described here. Three consecutive chromatographic steps on DE-52-Whatman cellulose were applied for isolation of two total plastocyanin (PC) fractions, oxidized [PC(II)] and reduced [PC(I)]. By chromatofocusing of PC(II) on Polybuffer exchanger 7...

journal_title：FEBS letters

authors： Dimitrov MI,Donchev AA,Egorov TA

更新日期：1990-06-04 00:00:00

abstract：:We have measured SGLT1 mRNA content and SGLT1-mediated glucose transport at different positions along the small intestine of control and streptozotocin diabetic rats and shown both parameters to be similar but higher in jejunal compared with ileal tissue. No such correlation was seen when comparing measurements of SGL...

journal_title：FEBS letters

authors： Dong R,Srai SK,Debnam E,Smith M

更新日期：1997-04-07 00:00:00

abstract：:The major mono- and disialogangliosides of the extensively characterized established human glioma line D54MG were isolated and purified from subcutaneous solid xenografts grown in athymic (nu/nu) mice. Structural determination showed that they belonged to the lactotetraosylceramide series. The sialyllactotetraosylcera...

journal_title：FEBS letters

authors： Månsson JE,Fredman P,Bigner DD,Molin K,Rosengren B,Friedman HS,Svennerholm L

更新日期：1986-05-26 00:00:00

abstract：:The solution conformation of the cardiac stimulatory sea anemone polypeptide anthopleurin-A has been characterised using distance geometry and restrained molecular dynamics calculations. A set of 253 approximate interproton distance restraints and 14 peptide backbone torsion angle restraints derived from two-dimension...

journal_title：FEBS letters

authors： Torda AE,Mabbutt BC,van Gunsteren WF,Norton RS

更新日期：1988-11-07 00:00:00

abstract：:A variety of plant tissues contain an uncoupling mitochondrial protein (PUMP), recently described and characterized by our group. In this study we show that the inhibition of PUMP activity in potato tuber mitochondria significantly increases mitochondrial H2O2 generation, while PUMP substrates, such as linoleic acid, ...

journal_title：FEBS letters

authors： Kowaltowski AJ,Costa AD,Vercesi AE

更新日期：1998-03-27 00:00:00

abstract：:The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Amon...

journal_title：FEBS letters

authors： Bluyssen HA,Lolkema MP,van Beest M,Boone M,Snijckers CM,Los M,Gebbink MF,Braam B,Holstege FC,Giles RH,Voest EE

更新日期：2004-01-02 00:00:00

abstract：:The entire structure of a 98 kb genomic region that abounds in genes related to magnetosome synthesis was first described in the Magnetospirillum sp. strain AMB-1. The deletion of this 98 kb genomic region and the circular form after excision from the chromosome was detected by PCR amplification. This strongly suggest...

journal_title：FEBS letters

authors： Fukuda Y,Okamura Y,Takeyama H,Matsunaga T

更新日期：2006-02-06 00:00:00

abstract：:Metal-binding peptides in proteolytic digest maps have been identified by matrix-assisted UV laser desorption time-of-flight mass spectrometry (LDTOF-MS). The plasma and milk metal transport protein chosen to demonstrate this process, histidine-rich glycoprotein (HRG), was purified and then digested with trypsin; the ...

journal_title：FEBS letters

authors： Hutchens TW,Nelson RW,Yip TT

更新日期：1992-01-13 00:00:00

abstract：:Sperm beta-acrosin activity is inhibited by suramin, a polysulfonated naphthylurea compound with therapeutic potential as a combined antifertility agent and microbicide. A kinetic analysis of enzyme inhibition suggests that three and four molecules of suramin bind to one molecule of ram and boar beta-acrosins respecti...

journal_title：FEBS letters

authors： Hermans JM,Haines DS,James PS,Jones R

更新日期：2003-06-05 00:00:00

abstract：:The farnesoid X receptor (FXR) is a nuclear receptor activated by bile acids (BAs). In response to ligand-binding, FXR regulates many genes involved in BA, lipid, and lipoprotein metabolism. To identify new FXR target genes, microarray technology was used to profile total RNA extracted from HepG2 cells treated with th...

journal_title：FEBS letters

authors： Sirvent A,Claudel T,Martin G,Brozek J,Kosykh V,Darteil R,Hum DW,Fruchart JC,Staels B

更新日期：2004-05-21 00:00:00

abstract：:Rab proteins are small GTPases involved in the regulation of membrane traffic. Rab5a has been shown to regulate transport in the early endocytic pathway. Here we report the isolation of cDNA clones encoding two highly related isoforms, Rab5b and Rab5c. The two proteins share with Rab5a all the structural features requ...

journal_title：FEBS letters

authors： Bucci C,Lütcke A,Steele-Mortimer O,Olkkonen VM,Dupree P,Chiariello M,Bruni CB,Simons K,Zerial M

更新日期：1995-06-05 00:00:00

abstract：:We have previously shown that the Mycoplasma mycoides glycine tRNA (anticodon UCC) effectively reads the codons GGU and GGC in violation of the classic codon reading rules. We have attempted to elucidate what structural elements in this tRNA molecule confer this translational property and in the course of this investi...

journal_title：FEBS letters

authors： Claesson C,Samuelsson T,Lustig F,Borén T

更新日期：1990-10-29 00:00:00

abstract：:Gene array profiling of RNA from cells engineered to express a dominant-negative version of the ETS family member transcription factor Pea3 (polyomavirus enhancer activator 3) identified WT1 as a candidate downstream gene. Given the co-expression of WT1 and Pea3 in developing kidney and breast tissue undergoing mesenc...

journal_title：FEBS letters

authors： Discenza MT,Vaz D,Hassell JA,Pelletier J

更新日期：2004-02-27 00:00:00