Binding properties of T4 gene 32 protein fragments carrying partially cleaved terminal domains.

abstract：:It has recently been discovered that glutathione-dependent formaldehyde dehydrogenase (FALDH) exhibits a strong S-nitrosoglutathione reductase activity. Plants use NO and S-nitrosothiols as signaling molecules to activate defense mechanisms. Therefore, it is interesting to investigate the regulation of FALDH by mechan...

journal_title：FEBS letters

authors： Díaz M,Achkor H,Titarenko E,Martínez MC

更新日期：2003-05-22 00:00:00

abstract：:Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a top A deletion mutant of Escherichia coli (DM800) that has a compensatory mutation in gyrB, lowering the activity of gyrase 10-fold,...

journal_title：FEBS letters

authors： McEachern F,Fisher LM

更新日期：1989-08-14 00:00:00

abstract：:L- and D-specific nicotinamide adenine dinucleotide (NAD)-dependent dehydrogenases map to the same structural protein superfamily as defined by the Structural Classification of Proteins (SCOP) and are based on the Rossmann fold type domains. A detailed classification of these domains is proposed using a novel diagnost...

journal_title：FEBS letters

authors： Kutzenko AS,Lamzin VS,Popov VO

更新日期：1998-02-13 00:00:00

abstract：:The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae...

journal_title：FEBS letters

authors： Kowaltowski AJ,Vercesi AE,Rhee SG,Netto LE

更新日期：2000-05-12 00:00:00

abstract：:Tumor necrosis factor (TNF) has been shown to inhibit the growth of some cell types and stimulate the proliferation of others by a mechanism that is not understood. In the present study, we investigated the effect of transfection of NIH-3T3 cells with either the basic fibroblast growth factor gene (bFGF) or the kaposi...

journal_title：FEBS letters

authors： Aggarwal BB,Pocsik E,Totpal K

更新日期：1995-09-18 00:00:00

abstract：:The TSH-induced cyclic AMP response was studied using a 3-year-old ovine thyroid cell line TSH-independent for growth: OVNIS 5H. The kinetics of cyclic AMP production was followed both in cell layers and in cell culture media, with or without phosphodiesterase inhibitor. It is noteworthy that following the first wave ...

journal_title：FEBS letters

authors： Fayet G,Aouani A,Hovsépian S

更新日期：1986-01-06 00:00:00

abstract：:Residues 370-383 (helix C) of the human nerve growth factor receptor (NGF-R) are highly similar to the sequence of the 14 residue wasp toxin, mastoparan. Both regions are predicted to form amphiphilic alpha-helices, as is the amino-terminal region of the third intracytoplasmic loop (i3) of the beta 2-adrenergic recept...

journal_title：FEBS letters

authors： Feinstein DL,Larhammar D

更新日期：1990-10-15 00:00:00

abstract：:The ATPase Cdc48 is required for membrane fusion and protein degradation. Recently it has been suggested that Cdc48 in a complex with Ufd1 and Npl4 acts as an ubiquitin-dependent chaperone. Here it is shown that recombinant Cdc48 alone can distinguish between the native and the non-native conformation of model substra...

journal_title：FEBS letters

authors： Thoms S

更新日期：2002-06-05 00:00:00

abstract：:Vibrio vulnificus is an opportunistic human pathogen that causes severe infections in susceptible individuals. While the components of the Escherichia coli phosphoenolpyruvate: sugar phosphotransferase system (PTS) have been shown to regulate numerous targets, little such information is available for the V. vulnificus...

journal_title：FEBS letters

authors： Kim YJ,Ryu Y,Koo BM,Lee NY,Chun SJ,Park SJ,Lee KH,Seok YJ

更新日期：2010-11-19 00:00:00

abstract：:The present study reported the isolation and characterization of a novel human secreted protein, named as hPAP21 (human protease-associated domain-containing protein, 21 kDa), encoded by the hypothetical gene chromosome 2 open reading frame 7 (C2orf7) that contains signal peptide in its N-terminus, without transmembra...

journal_title：FEBS letters

authors： Zhou YB,Liu F,Zhu ZD,Zhu H,Zhang X,Wang ZQ,Liu JH,Han ZG

更新日期：2004-10-22 00:00:00

abstract：:Niemann-pick type C1 (NPC1) is an endo/lysosomal membrane protein involved in intracellular cholesterol trafficking, and its luminal domain C is an essential endosomal receptor for Ebola and Marburg viruses. We have determined the crystal structure of glycosylated NPC1 luminal domain C and find all seven possible site...

journal_title：FEBS letters

authors： Zhao Y,Ren J,Harlos K,Stuart DI

更新日期：2016-03-01 00:00:00

abstract：:On the basis of its predicted homology to human telomerase reverse transcriptase (hTERT), a cDNA for Arabidopsis thaliana TERT (AtTERT) has now been isolated from cultured cells. The cDNA contains an open reading frame of 3372 bp, encoding a protein with a predicted size of 131 kDa and isoelectric point of 9.9. The At...

journal_title：FEBS letters

authors： Oguchi K,Liu H,Tamura K,Takahashi H

更新日期：1999-09-03 00:00:00

abstract：:Immunoblotting analysis was used to detect the microtubule-associated proteins present at different stages of rat brain development. Polyclonal antibodies were raised against the two main adult brain microtubule-associated proteins: MAP-2 (300 kDa) and TAU (60-70 kDa). Whatever the stage of development, anti-MAP-2 ser...

journal_title：FEBS letters

authors： Couchie D,Nunez J

更新日期：1985-09-02 00:00:00

abstract：:Anthrax is a severe bacterial infection that occurs when Bacillus anthracis spores gain access into the body and germinate in macrophages, causing septicemia and toxemia. Anthrax toxin is a binary A-B toxin composed of protective antigen (PA), lethal factor (LF), and edema factor (EF). PA mediates the entry of either ...

journal_title：FEBS letters

authors： Ascenzi P,Visca P,Ippolito G,Spallarossa A,Bolognesi M,Montecucco C

更新日期：2002-11-20 00:00:00

abstract：:Expression plasmids encoding native human preporenin and a mutant deleted in its entire prosegment were transfected into Chinese hamster ovary cells. The cells transfected with the expression plasmid of native preporenin secreted exclusively inactive prorenin, while the cells transfected with the mutant secreted the a...

journal_title：FEBS letters

authors： Nagahama M,Nakayama K,Hori H,Murakami K

更新日期：1989-12-18 00:00:00

abstract：:It is clear that G1-S phase control is exerted after the mouse embryo implants into the uterus 4.5 days after fertilization (E4.5); null mutants of genes that control cell cycle commitment such as max, rb (retinoblastoma), and dp1 are embryonic lethal after implantation with proliferation phenotypes. But, a number of ...

journal_title：FEBS letters

authors： Xie Y,Sun T,Wang QT,Wang Y,Wang F,Puscheck E,Rappolee DA

更新日期：2005-01-17 00:00:00

abstract：:Phosphoenolpyruvate carboxylases from various organisms contain two conserved lysine residues. In the C4 dicot Flaveria trinervia, one of these residues is Lys600. Converting this Lys600 to Arg600 or Thr600 mainly increased the Km values and but had minimal effect on the Vmax. The Km for PEP, Mg2+ increased by up to 3...

journal_title：FEBS letters

authors： Gao Y,Woo KC

更新日期：1995-11-13 00:00:00

abstract：:A colostral proline-rich polypeptide complex (PRP) consisting of over 30 peptides shows beneficial effects in Alzheimer's disease (AD) patients when administered in the form of sublinqual tablets called Colostrinin. The aim of the present studies was to investigate whether nanopeptide fragment of PRP (NP) - one of the...

journal_title：FEBS letters

authors： Janusz M,Woszczyna M,Lisowski M,Kubis A,Macała J,Gotszalk T,Lisowski J

更新日期：2009-01-05 00:00:00

abstract：:Proline-rich tyrosine kinase2 (Pyk2) is a cytoplasmic tyrosine kinase related to focal adhesion kinase. Pyk2 expression has been known to be restricted to neuronal and hematopoietic cells and Pyk2 tyrosine phosphorylation and its kinase activity is important for the function of monocytes/macrophages. In NB4 acute prom...

journal_title：FEBS letters

authors： Park MH,Park SY,Kim Y

更新日期：2008-02-06 00:00:00

abstract：:A short form of Escherichia coli translational initiation factor IF3, repeatedly found both in vivo and in vitro, lacking the positively charged N-terminal hexapeptide has been produced by mild trypsinization. The properties of this short form of IF3 have been studied. Compared to the long native form of the factor, t...

journal_title：FEBS letters

authors： Lammi M,Pon CL,Gualerzi CO

更新日期：1987-05-04 00:00:00

abstract：:We investigated 38 cases of B-cell chronic lymphocytic leukemia (B-CLL) for the presence of non-productive rearrangements in the S(mu) regions and defined for the first time the molecular nature of these rearrangements. Southern blot analysis revealed S(mu) region rearrangements in 13 cases (34%) and polymerase chain ...

journal_title：FEBS letters

authors： Nardini E,Rizzi S,Capello D,Vitolo U,Gaidano G,Menard S,Balsari A

更新日期：2002-05-08 00:00:00

abstract：:Microinjection of human liver mRNA from a patient homozygous for alpha 1-antitrypsin deficiency (PiZZ) into Xenopus oocytes led to a 2--10-fold increase in lysosomal activity. Stimulation of lysosomal activity was not observed when mRNA from a normal human liver (alpha 1-antitrypsin PiMM), or water was injected into t...

journal_title：FEBS letters

authors： Bathurst IC,Errington DM,Foreman RC,Judah JD,Carrell RW

更新日期：1985-04-22 00:00:00

abstract：:The pl6INK4a tumor suppressor negatively regulates progression through the G1 phase of the mammalian cell cycle. To mimic the downmodulation of p16INK4a commonly seen in cancer, we designed and characterized a hammerhead ribozyme against exon E1alpha of the murine pl6INK4a transcript. Stable expression of the ribozyme...

journal_title：FEBS letters

authors： Nylandsted J,Rohde M,Bartek J,Strauss M

更新日期：1998-09-25 00:00:00

abstract：:Coagulation factor Va is an essential cofactor which combines with the serine protease factor Xa on a phospholipid surface to form the prothrombinase complex. In the present study, the structure of factor Va interacting with lipid surfaces containing phosphatidylserine was studied by electron microscopy. Two-dimension...

journal_title：FEBS letters

authors： Stoylova S,Mann KG,Brisson A

更新日期：1994-09-12 00:00:00

abstract：:The structure of the Fab fragment of the monoclonal antibody MAK 33 (kappa/IgG1) at pH 2 was characterized. Spectroscopic and kinetic analysis revealed a molten globule-like state, characterized by elements of secondary structure but less defined tertiary contacts than in the native state. However, some aromatic side ...

journal_title：FEBS letters

authors： Lilie H,Buchner J

更新日期：1995-03-27 00:00:00

abstract：:We found that miR-96 is overexpressed in glioma, and its level inversely correlates with the survival of patients. The reduction in miR-96 abundance suppresses the proliferation and colony formation of glioma cells. The tumorigenicity of U-87 MG cells is reduced by miR-96 silencing. miR-96 contributes to the activatio...

journal_title：FEBS letters

authors： Yan Z,Wang J,Wang C,Jiao Y,Qi W,Che S

更新日期：2014-08-25 00:00:00

abstract：:Single rat hepatocytes microinjected with aequorin show free Ca oscillations when stimulated with Ca(2+)-mobilizing hormones. We show here that an inhibitor of diacylglycerol kinase (R 59 022) and an analogue of native diacylglycerol (diC8) inhibit free Ca oscillations induced by phenylephrine and vasopressin. These r...

journal_title：FEBS letters

authors： Sanchez-Bueno A,Cobbold PH

更新日期：1992-11-02 00:00:00

abstract：:Even though the plant photoreceptors cryptochromes were discovered more than 20 years ago, the mechanism through which they transduce light signals to their partner molecules such as COP1 (Constitutive Photomorphogenic 1) or SPA1 (Suppressor of Phytochrome A) still remains to be established. We propose that a negative...

journal_title：FEBS letters

authors： Müller P,Bouly JP

更新日期：2015-01-16 00:00:00

abstract：:Mitochondrial thioredoxin (mtTrx) can be oxidized in response to inducers of oxidative stress; yet the functional consequences of the oxidation have not been determined. This study evaluated the redox status of mtTrx and its association to oxidant-induced apoptosis. Results showed that mtTrx was oxidized after exposur...

journal_title：FEBS letters

authors： Chen Y,Cai J,Jones DP

更新日期：2006-12-11 00:00:00

abstract：:The possible in vivo interaction of the Nicotiana tabacum agglutinin (Nictaba) with endogenous glycoproteins was corroborated using a combination of confocal/electron microscopy of an EGFP-Nictaba fusion protein expressed in tobacco Bright Yellow-2 (BY-2) cells and biochemical analyses. In vitro binding studies demons...

journal_title：FEBS letters

authors： Lannoo N,Peumans WJ,Pamel EV,Alvarez R,Xiong TC,Hause G,Mazars C,Van Damme EJ

更新日期：2006-11-27 00:00:00