Abstract:
:Adrenomedullin, a potently hypotensive peptide isolated from human pheochromocytoma, is known to elicit a rise in cAMP levels within mammalian endothelial and smooth muscle cells. Until now, however, little has been known about the adrenomedullin receptor. Recently, a group called receptor activity-modifying proteins that complex with the calcitonin receptor-like receptor, and thereby regulate its transport and ligand specificity, were identified. Here we show that mRNA for both the calcitonin receptor-like receptor and the receptor activity-modifying protein 2, but not the receptor activity-modifying protein 1 or receptor activity-modifying protein 3, are expressed in human endothelial and vascular smooth muscle cells. We also found that adrenomedullin increased cAMP levels in HeLa EBNA and 293 EBNA cells, expressing both the receptor activity-modifying protein 2 and calcitonin receptor-like receptor proteins. Thus, the receptor activity-modifying protein 2/calcitonin receptor-like receptor complex apparently serves as a functional adrenomedullin receptor in human endothelial and vascular smooth muscle cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kamitani S,Asakawa M,Shimekake Y,Kuwasako K,Nakahara K,Sakata Tdoi
10.1016/s0014-5793(99)00358-0keywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
111-4issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00358-0journal_volume
448pub_type
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