Abstract:
:Prodigiosin 25-C inhibited the accumulation of 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine and acridine orange in the acidic compartments of baby hamster kidney cells with little perturbation of cellular ATP levels. In rat liver lysosomes, prodigiosin 25-C inhibited the proton pump activity with an IC50 of approximately 30 nM, but did not affect ATPase activity up to 1 microM. It also delayed the transport of vesicular stomatitis virus G protein and induced a drastic swelling of Golgi apparatus and mitochondria. These results indicate that prodigiosin 25-C raises the pH of acidic compartments through inhibition of the proton pump activity of vacuolar type H(+)-ATPase, thereby causing the functional and morphological changes to the Golgi apparatus.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kataoka T,Muroi M,Ohkuma S,Waritani T,Magae J,Takatsuki A,Kondo S,Yamasaki M,Nagai Kdoi
10.1016/0014-5793(94)01446-8subject
Has Abstractpub_date
1995-02-06 00:00:00pages
53-9issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)01446-8journal_volume
359pub_type
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