Abstract:
:Human kallikrein 8 (KLK8) is a member of the human kallikrein gene family of serine proteases, and its protein, hK8, has recently been suggested to serve as a new ovarian cancer marker. To gain insights into the physiological role of hK8, the active recombinant enzyme was obtained in a pure state for biochemical and enzymatic characterizations. hK8 had trypsin-like activity with a strong preference for Arg over Lys in the P1 position, and its activity was inhibited by typical serine protease inhibitors. The protease degraded casein, fibronectin, gelatin, collagen type IV, fibrinogen, and high-molecular-weight kininogen. hK8 also converted human single-chain tissue-type plasminogen activator (65 kDa) to its two-chain form (32 and 33 kDa) by specifically cleaving the peptide bond Arg275-Ile276. This conversion resulted in a drastic increase in the activity of the activator toward the fluorogenic substrate Pyr-Gly-Arg-MCA and plasminogen in the absence of fibrin. Our findings suggest that hK8 may be implicated in ECM protein degradation in the area surrounding hK8-producing cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rajapakse S,Ogiwara K,Takano N,Moriyama A,Takahashi Tdoi
10.1016/j.febslet.2005.11.039keywords:
subject
Has Abstractpub_date
2005-12-19 00:00:00pages
6879-84issue
30eissn
0014-5793issn
1873-3468pii
S0014-5793(05)01402-Xjournal_volume
579pub_type
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