Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins.

Abstract:

:Human kallikrein 8 (KLK8) is a member of the human kallikrein gene family of serine proteases, and its protein, hK8, has recently been suggested to serve as a new ovarian cancer marker. To gain insights into the physiological role of hK8, the active recombinant enzyme was obtained in a pure state for biochemical and enzymatic characterizations. hK8 had trypsin-like activity with a strong preference for Arg over Lys in the P1 position, and its activity was inhibited by typical serine protease inhibitors. The protease degraded casein, fibronectin, gelatin, collagen type IV, fibrinogen, and high-molecular-weight kininogen. hK8 also converted human single-chain tissue-type plasminogen activator (65 kDa) to its two-chain form (32 and 33 kDa) by specifically cleaving the peptide bond Arg275-Ile276. This conversion resulted in a drastic increase in the activity of the activator toward the fluorogenic substrate Pyr-Gly-Arg-MCA and plasminogen in the absence of fibrin. Our findings suggest that hK8 may be implicated in ECM protein degradation in the area surrounding hK8-producing cells.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Rajapakse S,Ogiwara K,Takano N,Moriyama A,Takahashi T

doi

10.1016/j.febslet.2005.11.039

keywords:

subject

Has Abstract

pub_date

2005-12-19 00:00:00

pages

6879-84

issue

30

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(05)01402-X

journal_volume

579

pub_type

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