In the rat, tumor necrosis factor alpha administration results in an increase in both UCP2 and UCP3 mRNAs in skeletal muscle: a possible mechanism for cytokine-induced thermogenesis?

abstract：:Reversible phosphorylation of myosin subunits is observed in almost all eukaryotic cells. The data concerning sites and effects of phosphorylation on actin-activated ATPase activity of myosin and on its filament formation are described. These observations are discussed in terms of possible evolutionary trends and rule...

journal_title：FEBS letters

authors： Kuźnicki J

更新日期：1986-08-18 00:00:00

abstract：:It has been suggested that alpha-dystroglycan links the dystrophin-associated protein complex and extracellular matrix and that the absence of dystrophin and alpha-dystroglycan in Duchenne muscular dystrophy (DMD) may lead to the breakdown of this linkage. In the present study, myotubes from DMD patients and murine X-...

journal_title：FEBS letters

authors： Angoli D,Corona P,Baresi R,Mora M,Wanke E

更新日期：1997-05-26 00:00:00

abstract：:Activities of prevalent protein phosphatases decreased by nearly 95% and those of individual protein kinases were differentially reduced at low temperature. Inhibition of phosphatase activity at temperatures below 12 degrees C resulted in marked hyperphosphorylation of a 58-kDa protein (PP58). The temperature threshol...

journal_title：FEBS letters

authors： Monroy AF,Labbé E,Dhindsa RS

更新日期：1997-06-30 00:00:00

abstract：:Mature dermal glands of Xenopus laevis contain storage granules with a characteristic ellipsoid shape. A few major proteins are present in these granules, including two with the same amino-terminal sequence and apparent molecular masses of 26 and 28 kDa. Using antibodies against these proteins, positive clones were is...

journal_title：FEBS letters

authors： Berger H,Kreil G

更新日期：1989-06-05 00:00:00

abstract：:A number of human diseases can be linked to aberrations in protein folding which cause an imbalance in protein homeostasis. Molecular chaperones, including heat shock proteins, act to assist protein folding, stability and activity in the cell. Attention has begun to focus on modulating the expression and/or activity o...

journal_title：FEBS letters

authors： Powers MV,Workman P

更新日期：2007-07-31 00:00:00

abstract：:The effect of artificial model membranes on the complement system was investigated. Incubation of the model membranes with human serum resulted in consumption of complement haemolytic activity when phosphatidylserine-containing vesicles were used. The activation of the complement system appeared to proceed through the...

journal_title：FEBS letters

authors： Comis A,Easterbrook-Smith SB

更新日期：1986-03-03 00:00:00

abstract：:Hemin stimulates cAMP production in human peripheral blood mononuclear cells (PBMC). The kinetics are similar to that of hormone-induced cAMP generation, namely a rapid effect followed by a desensitization phase. Several experimental findings suggest that prostaglandins do not mediate this effect. First, macrophage de...

journal_title：FEBS letters

authors： Lander HM,Levine DM,Novogrodsky A

更新日期：1992-06-01 00:00:00

abstract：:We tested the effect of melatonin on membrane fluidity in microsomes of a rat liver model in which lipid peroxidation was induced by the addition of FeCl3, ADP and NADPH. Membrane fluidity was monitored using fluorescence spectroscopy and lipid peroxidation was estimated by quantifying malonaldehyde (MDA)+4-hydroxyalk...

journal_title：FEBS letters

authors： García JJ,Reiter RJ,Guerrero JM,Escames G,Yu BP,Oh CS,Muñoz-Hoyos A

更新日期：1997-05-26 00:00:00

abstract：:The yeast gene, ACP1, encoding the mitochondrial acyl carrier protein, was deleted by gene replacement. The resulting acp1-deficient mutants had only 5-10% of the wild-type lipoic acid content remaining, and exhibited a respiratory-deficient phenotype. Upon meiosis, the lipoate deficiency co-segregated with the acp1 d...

journal_title：FEBS letters

authors： Brody S,Oh C,Hoja U,Schweizer E

更新日期：1997-05-19 00:00:00

abstract：:Rat liver fructose 1,6-bisphosphatase can be protected against partial inactivation by N-ethylmaleimide by low concentrations of fructose 2,6-bisphosphate or high concentrations of fructose 1,6-bisphosphate. The partially inactivated enzyme has a much reduced sensitivity to high substrate inhibition and has lost the s...

journal_title：FEBS letters

authors： Meek DW,Nimmo HG

更新日期：1983-08-22 00:00:00

abstract：:We previously showed that the post-translational cleavage of alphav subunit is essential for integrin-dependent signalling and cell adhesion. Here, we report that blocking alphav subunit cleavage by expression of alpha1-PDX, a convertase inhibitor, modified the capacity of cells to change shape, via a remodelling of t...

journal_title：FEBS letters

authors： Berthet V,Rigot V,Nejjari M,Marvaldi J,Luis J

更新日期：2004-01-16 00:00:00

abstract：:Organelles have been studied traditionally as single units, but a novel concept is now emerging: each organelle has distinct functional zones that regulate specific functions. The Golgi apparatus seems to have various zones, including zones for: glycosylphosphatidylinositol-anchored proteins; proteoglycan, mucin and l...

journal_title：FEBS letters

authors： Sasaki K,Yoshida H

更新日期：2019-09-01 00:00:00

abstract：:Treatment of Chinese hamster ovary K1 cells with phosphatidylserine (PS) caused typical apoptosis with distinct morphological and biochemical features in a dose- and time-dependent manner. However, unlike camptothecin-induced apoptosis, changes in mitochondrial transmembrane potential were not observed. In addition, c...

journal_title：FEBS letters

authors： Miyato Y,Ibuki Y,Ohyama H,Yamada T,Goto R

更新日期：2001-08-24 00:00:00

abstract：:Reaction of ubiquinone in the high-affinity quinone-binding site (QH) in bo-type ubiquinol oxidase from Escherichia coli was revealed by EPR and optical studies. In the QH site, ubiquinol was shown to be oxidized to ubisemiquinone and to ubiquinone, while no semiquinone signal was detected in the oxidase isolated from...

journal_title：FEBS letters

authors： Sato-Watanabe M,Itoh S,Mogi T,Matsuura K,Miyoshi H,Anraku Y

更新日期：1995-10-30 00:00:00

abstract：:Muscarinic acetylcholine receptors purified from porcine atrium were phosphorylated, depending on the presence of agonists, by a protein kinase partially purified from porcine brain, which had similar properties to the beta-adrenergic receptor kinase. GTP-binding regulatory proteins (Go) had dual effects on the phosph...

journal_title：FEBS letters

authors： Haga K,Haga T

更新日期：1990-07-30 00:00:00

abstract：:Cyanobacteria possess genes encoding phytochrome-related proteins. We used a DNA microarray approach to evaluate the impact of the phytochromes Cph1 and Cph2 on red light (R)- and far-red light (FR)-dependent gene expression in the unicellular cyanobacterium Synechocystis sp. PCC 6803. In cells of wild-type and phytoc...

journal_title：FEBS letters

authors： Hübschmann T,Yamamoto H,Gieler T,Murata N,Börner T

更新日期：2005-03-14 00:00:00

abstract：:A point mutation (Ala-589 to Thr) in the transmembrane protein of the human immunodeficiency virus type 1 (HIV-1) has been shown to decrease the sensitivity of the virus to the neutralizing effect of human HIV-1 specific antibodies [(1990) J. Virol. 64, 3240-3248]. Here 17-residue peptides with the parental and mutant...

journal_title：FEBS letters

authors： Klasse J,Pipkorn R,Blomberg J,Han KH,Hilton B,Ferretti JA

更新日期：1993-05-24 00:00:00

abstract：:The interaction of Bordetella pertussis adenylate cyclase toxin (CyaA) with complement receptor 3 (CR3, CD11b/CD18) involves N-linked oligosaccharide chains. To investigate the relative importance of the individual N-glycans of CR3 for toxin activity, the asparagine residues of the consensus N-glycosylation sites of C...

journal_title：FEBS letters

authors： Hasan S,Osickova A,Bumba L,Novák P,Sebo P,Osicka R

更新日期：2015-01-30 00:00:00

abstract：:Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DNA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres. We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80. Two reac...

journal_title：FEBS letters

authors： Song K,Jung D,Jung Y,Lee SG,Lee I

更新日期：2000-09-08 00:00:00

abstract：:Transcription mediators are evolutionarily conserved from yeast to human. We previously reported the specific in vivo roles of mediators during development. Transcriptional mediators including med-6, med-7, and med-10 were shown to be involved in the regulated transcription of specific genes, but not in the transcript...

journal_title：FEBS letters

authors： Kwon JY,Kim-Ha J,Lee BJ,Lee J

更新日期：2001-11-23 00:00:00

abstract：:In human platelet membranes enhanced tyrosine phosphorylation of certain proteins was observed when Zn2+ instead of Mg2+ or Mn2+ was used as a divalent cation for the kinase reaction. An enhanced level of phosphate incorporation into tyrosine residues occurred into a 68 kDa polypeptide besides the 45 kDa and 105 kDa p...

journal_title：FEBS letters

authors： Findik D,Presek P

更新日期：1988-08-01 00:00:00

abstract：:The bipartite human interleukin-4 (IL-4) receptor was functionally expressed in murine pro-B cells and activated by human IL-4 to evoke intracellular signaling. Mutual association of signal transducing proteins within the receptor complex was then studied in dependence of ligand stimulation. Besides ligand-induced rec...

journal_title：FEBS letters

authors： Erhardt I,Lischke A,Sebald W,Friedrich K

更新日期：1998-11-13 00:00:00

abstract：:A cell-free system derived from carrot cell cytosol extract has been developed for reassembling nuclear structure around the added demembranated sperm chromatin of Xenopus. Morphological evidence suggests that reassembled nuclei display the typical characteristics of normal eukaryotic nuclei, such as double-layered nu...

journal_title：FEBS letters

authors： Zhao Y,Liu X,Wu M,Tao W,Zhai Z

更新日期：2000-09-01 00:00:00

abstract：:In thrombin-stimulated platelets alpha IIb beta 3 integrin engagement triggers both phosphatidylinositol 3',4'-bisphosphate synthesis and calpain activation. We checked the possible involvement of calpains in phosphatidylinositol 3-kinase signalling pathway using a cell permeant specific inhibitor of calpains, calpept...

journal_title：FEBS letters

authors： Montsarrat N,Racaud-Sultan C,Mauco G,Plantavid M,Payrastre B,Breton-Douillon M,Chap H

更新日期：1997-03-03 00:00:00

abstract：:Laser flash-induced photovoltage responses of the D85S and D85T mutants as well as of the wild-type acid blue form are similar and reflect intraprotein charge redistribution caused by retinal isomerization. The Cl- -induced transition of all of these blue forms into purple ones is accompanied by the appearance of elec...

journal_title：FEBS letters

authors： Kalaidzidis IV,Kaulen AD

更新日期：1997-12-01 00:00:00

abstract：:This review focuses on the factors that regulate the proliferation of pancreatic islet beta-cells in vitro, and in particular on the intracellular pathways that convey the mitogenic signal into a proliferative response. Substances as diverse as nutrients, polypeptides, cytokines, adrenergic agents, lithium, phorbol es...

journal_title：FEBS letters

authors： Sjöholm A

更新日期：1992-10-19 00:00:00

abstract：:The cholecystokinin (CCK) receptor in purified plasma membranes prepared from mouse pancreatic acini had a binding affinity of 1.8 nM, an acid pH optimum between 6.0 and 6.5, and an analog specificity of CCK8 greater than CCK33 greater than desulphated CCK8 greater than CCK4. Binding of CCK to its receptor was abolish...

journal_title：FEBS letters

authors： Sakamoto C,Williams JA,Wong KY,Goldfine ID

更新日期：1983-01-10 00:00:00

abstract：:Glycogen synthase from human and bovine polymorphonuclear leukocytes was purified to homogeneity. Rabbit antisera were raised against the two glycogen synthases and used for immunochemical analysis. Western blotting analysis showed that the subunit of glycogen synthase in crude homogenates of human and bovine leukocyt...

journal_title：FEBS letters

authors： Rasmussen LH,Juhl H,Esmann V,Petersen HU

更新日期：1988-03-28 00:00:00

abstract：:Prolonged incubation of membrane fragments containing homogeneous (Na+ + K+)-ATPase with Mg2+, K+ and VO-3 at 4 degrees C resulted in formation of two-dimensional crystals of this enzyme with unit cell parameters: a = 66 A, b = 118 A, gamma = 108 degrees. The crystals correspond to the two-sided plane group p21. By co...

journal_title：FEBS letters

authors： Ovchinnikov YuA,Demin VV,Barnakov AN,Kuzin AP,Lunev AV,Modyanov NN,Dzhandzhugazyan KN

更新日期：1985-10-07 00:00:00

abstract：:Incubation of rat skeletal muscle mitochondria with the nitric oxide generator, S-nitrosoglutathione (GSNO) reversibly inhibited oxygen utilisation with all substrates tested. The visible absorption spectra of the inhibited mitochondria showed that cytochromes c+c1, b and a+a3 were reduced, indicating a block at the d...

journal_title：FEBS letters

authors： Cleeter MW,Cooper JM,Darley-Usmar VM,Moncada S,Schapira AH

更新日期：1994-05-23 00:00:00