Golgi stress response and organelle zones.

abstract：:Interactions of U2 snRNA with anti-Sm precipitable proteins in RNA-free cytoplasmic complexes were analyzed. U2 snRNA was found to bind specifically with proteins in the 6S complexes but not in the 20S complexes. The binding activity was preserved using U2 snRNA having a mutated Sm binding site. Label-transfer experim...

journal_title：FEBS letters

authors： Fury MG,Andersen J

更新日期：1997-03-03 00:00:00

abstract：:The sulfoxide Ro 18-5364, a potential metabolite of the IND Ro 18-5362, is a powerful inhibitor of gastric mucosal (H+ + K+)-ATPase, decreasing enzymatic activity with an apparent Ki of 0.1 microM. Exposure of Ro 18-5364-treated gastric membranes to dithiothreitol fully restored (H+ + K+)-ATPase activity. ATP protecte...

journal_title：FEBS letters

authors： Sigrist-Nelson K,Müller RK,Fischli AE

更新日期：1986-03-03 00:00:00

abstract：:Bacterial fructosyltransferase enzymes belonging to glycoside hydrolase family 68 (GH68) are not known to require a metal cofactor. Here, we show that Ca2+ ions play an important structural role in the Lactobacillus reuteri 121 levansucrase (Lev) and inulosucrase (Inu) enzymes. Analysis of the Bacillus subtilis Lev 3D...

journal_title：FEBS letters

authors： Ozimek LK,Euverink GJ,van der Maarel MJ,Dijkhuizen L

更新日期：2005-02-14 00:00:00

abstract：:Mitochondrial fatty acid synthesis is catalyzed by a dissociated fatty acid synthase similar to those of plant plastids and bacteria. The crystal structure of a mitochondrial beta-ketoacyl-[acyl carrier protein] synthase (mtKAS), namely that from Arabidopsis thaliana, has been determined for the first time. This enzym...

journal_title：FEBS letters

authors： Olsen JG,Rasmussen AV,von Wettstein-Knowles P,Henriksen A

更新日期：2004-11-05 00:00:00

abstract：:Trace amounts of gangliosides were found in rabbit skeletal muscle sarcoplasmic reticulum and their main part was shown, by high performance thin layer chromatography. to be GM3. Addition of GM3 to the soybean phospholipids used for reconstitution of proteoliposomes markedly increased ATP hydrolysis as well as Ca2+ up...

journal_title：FEBS letters

authors： Wang LH,Tu YP,Yang XY,Tsui ZC,Yang FY

更新日期：1996-06-17 00:00:00

abstract：:The nef gene of human and simian immunodeficiency virus is a key factor in acquired immunodeficiency syndrome pathogenesis and virus replication. Several Nef-induced phenomena, including the down-regulation of CD4 molecule, have been previously reported. In this study, we have identified and cloned a novel cellular pr...

journal_title：FEBS letters

authors： Fukushi M,Dixon J,Kimura T,Tsurutani N,Dixon MJ,Yamamoto N

更新日期：1999-01-08 00:00:00

abstract：:In this work we have investigated the role of specific peptide sequences for glucose-inactivation of the yeast isocitrate lyase. Thus, different fragments of the ICL1 coding region were fused to the lacZ gene of E. coli to provide a reporter construction. Determinations of beta-galactosidase activities indicated that ...

journal_title：FEBS letters

authors： Ordiz I,Herrero P,Rodicio R,Moreno F

更新日期：1995-07-03 00:00:00

abstract：:Annexin V is an alpha-helical protein which shows anticoagulatory and antiinflammatory activity. It is supposed to be involved in membrane fusion and exocytosis. In this study acid-induced equilibrium unfolding of the human annexin V is investigated by fluorescence and circular dichroism spectroscopy. The spectroscopi...

journal_title：FEBS letters

authors： Beermann ofm cap BB,Hinz HJ,Hofmann A,Huber R

更新日期：1998-02-20 00:00:00

abstract：:Several peptidic and non-peptidic factors can modulate Na,K-ATPase activity, among them mainly inhibitors of this enzyme, ouabain being the most effective. In a very few cases only, activation of Na,K-ATPase by endogenous factors has been recorded. We have investigated the effect on Na,K-ATPase of a novel regulatory p...

journal_title：FEBS letters

authors： Kairane C,Zilmer M,Mutt V,Sillard R

更新日期：1994-05-23 00:00:00

abstract：:Chemokines play a key role in modulating leukocyte functions at sites of inflammation. To assess chondrocyte contribution to the chemotactic environment of inflamed joints the intracellular content of CC and CXC chemokines was investigated. IL-8, GROalpha, MCP-1, RANTES, MIP-1alpha and MIP-1beta expression was evaluat...

journal_title：FEBS letters

authors： Borzi RM,Mazzetti I,Macor S,Silvestri T,Bassi A,Cattini L,Facchini A

更新日期：1999-07-23 00:00:00

abstract：:Treatment of yeast phosphoglycerate kinase (PGK) with trypsin results in a fourfold increase in the Vmax of this enzyme, without affecting the Km. This activation is shown to be due to the removal of the C-terminal lysine residue. The C-terminal sequence folds back over the N-terminal domain and contacts the extreme N...

journal_title：FEBS letters

authors： Adams B,Fowler R,Hudson M,Pain RH

更新日期：1996-04-29 00:00:00

abstract：:Retroviral proteinases (PRs) are essential for retrovirus infectivity but the mechanism of their activity regulation is poorly understood. We investigated possible involvement in this process of the C-terminal domain (CTD) of betaretroviral PRs. We found that the presence of CTD attenuates proteolytic activity of Maso...

journal_title：FEBS letters

authors： Svec M,Bauerová H,Pichová I,Konvalinka J,Strísovský K

更新日期：2004-10-08 00:00:00

abstract：:A full-length cDNA coding for troponin-C isolated from an adult rabbit fast skeletal muscle library has been sequenced and expressed in an E. coli host. The amino acid sequence derived from the coding region agrees with the published protein sequence except that the first two residues are reversed. The expressed prote...

journal_title：FEBS letters

authors： Chen Q,Taljanidisz J,Sarkar S,Tao T,Gergely J

更新日期：1988-02-08 00:00:00

abstract：:Understanding specific cargo distribution in differentiated cells is a major challenge. Trafficking kinesin proteins (TRAKs) are kinesin adaptors. They bind the cargo binding domain of kinesin-1 motor proteins forming a link between the motor and their cargoes. To refine the TRAK1/2 binding sites within the kinesin-1 ...

journal_title：FEBS letters

authors： Randall TS,Moores C,Stephenson FA

更新日期：2013-11-29 00:00:00

abstract：:Aminoacyl-tRNA synthetases, a group of enzymes catalyzing aminoacyl-tRNA formation, may possess inherent editing activity to clear mistakes arising through the selection of non-cognate amino acid. It is generally assumed that both editing substrates, non-cognate aminoacyl-adenylate and misacylated tRNA, are hydrolyzed...

journal_title：FEBS letters

authors： Gruic-Sovulj I,Rokov-Plavec J,Weygand-Durasevic I

更新日期：2007-10-30 00:00:00

abstract：:Using sensitive methods of sequence analysis including hydrophobic cluster analysis, we report here a hitherto undescribed family of modules, the BAH (bromo-adjacent homology) family, which includes proteins such as eukaryotic DNA (cytosine-5) methyltransferases, the origin recognition complex 1 (Orc1) proteins, as we...

journal_title：FEBS letters

authors： Callebaut I,Courvalin JC,Mornon JP

更新日期：1999-03-05 00:00:00

abstract：:HepG2 cells, stably transfected with MDR1 cDNA, encoding the P-glycoprotein multidrug resistance efflux pump, display an altered sphingolipid composition compared to control cells, stably transfected with empty vector. The MDR1 overexpressing cells display a approximately 3-fold increased level of lactosylceramide and...

journal_title：FEBS letters

authors： Hummel I,Klappe K,Kok JW

更新日期：2005-06-20 00:00:00

abstract：:Replacements of Asp-295 by Asn (D295N) and Glu (D295E) decreased the catalytic center activity of Leuconostoc mesenteroides sucrose phosphorylase to about 0.01% of the wild-type level (k(cat)=200s(-1)). Glucosylation and deglucosylation steps of D295N were affected uniformly, approximately 10(4.3)-fold, and independen...

journal_title：FEBS letters

authors： Mueller M,Nidetzky B

更新日期：2007-04-03 00:00:00

abstract：:The alternative nitrogenase of Rhodobacter capsulatus, isolated from a nifHDK deletion mutant, has been purified to near homogeneity and identified as an 'iron only' nitrogenase. The dithionite-reduced component 1 ('FeFe protein') of this enzyme showed an EPR spectrum consisting of two components: a minor S = 1/2 sign...

journal_title：FEBS letters

authors： Müller A,Schneider K,Knüttel K,Hagen WR

更新日期：1992-05-25 00:00:00

abstract：:The relationship between ground state cis-trans isomerization and protonation state is explored for a model green fluorescent protein chromophore, 4-hydroxybenzylidene-1,2-dimethylimidazolinone (HBDI). We find that the protonation state has only a modest effect on the free energy differences between cis and trans isom...

journal_title：FEBS letters

authors： He X,Bell AF,Tonge PJ

更新日期：2003-08-14 00:00:00

abstract：:Alix and its homologs are involved in various phenomena such as endosomal protein-sorting and adaptation to stress conditions. In this study, we found that development of Dictyostelium discoideum Alix (DdAlix) deletion mutant (alx-) cells was impaired in alkaline pH environments. The fruiting body formation efficiency...

journal_title：FEBS letters

authors： Ohkouchi S,Saito H,Aruga F,Maeda T,Shibata H,Maki M

更新日期：2005-03-14 00:00:00

abstract：:Increased expression of low voltage-activated, T-type Ca(2+) channels has been correlated with a variety of cellular events including cell proliferation and cell cycle kinetics. The recent cloning of three genes encoding T-type alpha(1) subunits, alpha(1G), alpha(1H) and alpha(1I), now allows direct assessment of thei...

journal_title：FEBS letters

authors： Chemin J,Monteil A,Briquaire C,Richard S,Perez-Reyes E,Nargeot J,Lory P

更新日期：2000-07-28 00:00:00

abstract：:Islet amyloid polypeptide (IAPP) is the 37-amino acid peptide subunit of amyloid found in pancreatic islets of type 2 diabetic patients and in insulinomas. Recently, we isolated the human gene encoding IAPP [(1988) FEBS Lett. 239, 227-232]. We now report the nucleotide sequences of a human insulinoma cDNA encoding a c...

journal_title：FEBS letters

authors： Mosselman S,Höppener JW,Lips CJ,Jansz HS

更新日期：1989-04-10 00:00:00

abstract：:The gene for titin, a 4MDa myofibrillar protein, was analysed in golden hamster DNAs from different sources, using human cDNA probes and PCR. In the DNA from the BHK-21-Bi subline of baby hamster kidney cells, extended sequences coding for Z-line associated domains were missing, indicating a deletion that renders titi...

journal_title：FEBS letters

authors： Jäckel M,Witt C,Antonova O,Curdt I,Labeit S,Jockusch H

更新日期：1997-05-12 00:00:00

abstract：:In this work, we investigated electron transport around the photosynthetic pigment-protein complex of Photosystem I (PS I) mediated by external high-potential electron carrier 2,3-dichloro-1,4-naphtoquinone (Cl2 NQ) and ascorbate. It has been demonstrated that the oxidized species of Cl2 NQ and ascorbate serve as inte...

journal_title：FEBS letters

authors： Petrova AA,Trubitsin BV,Boskhomdzhieva BK,Semenov AY,Tikhonov AN

更新日期：2018-07-01 00:00:00

abstract：:The presence of G-proteins in the eyespot fraction of Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTP gamma[35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' alpha-subunit. Cross-reaction with a 24-kDa protein is...

journal_title：FEBS letters

authors： Korolkov SN,Garnovskaya MN,Basov AS,Chunaev AS,Dumler IL

更新日期：1990-09-17 00:00:00

abstract：:Co-expression of the two genes encoding the alpha- and beta-subunit of the Thermoplasma acidophilum thermosome in Escherichia coli yielded fully assembled hetero-oligomeric complexes (alpha+beta). Surprisingly, also separate expression of both genes resulted in formation of hexadecameric complexes (alpha, beta) in the...

journal_title：FEBS letters

authors： Waldmann T,Nitsch M,Klumpp M,Baumeister W

更新日期：1995-11-27 00:00:00

abstract：:γ-Glutamyl kinase (GK; the PRO1 gene product) is a key enzyme in the Saccharomyces cerevisiae proline biosynthesis pathway. Δpro1 cells are more sensitive to various stresses than wild-type cells, suggesting that GK has an alternative function independent of proline biosynthesis. We show that PRO1 genetically interact...

journal_title：FEBS letters

authors： Tatehashi Y,Watanabe D,Takagi H

更新日期：2016-09-01 00:00:00

abstract：:SSTR3, a somatostatin (SST) receptor, is an adenylyl cyclase (AC)-inhibiting receptor. To assign the G-protein alpha-subunit (G alpha) linked to this receptor, we created a novel reporter system which utilizes the well-established facts that the C-terminal 5 residues of G alpha are the receptor contact site and G alph...

journal_title：FEBS letters

authors： Komatsuzaki K,Murayama Y,Giambarella U,Ogata E,Seino S,Nishimoto I

更新日期：1997-04-07 00:00:00

abstract：:The structure of the Ala38 variant of yeast iso-1-cytochrome c, in which the previously unchanged Arg38 has been replaced, has been characterised by NMR. The NMR data indicate that the structure of the Ala38 variant is very similar to that of the wild type protein. In particular, the heme environment and interactions ...

journal_title：FEBS letters

authors： Thurgood AG,Pielak GJ,Cutler RL,Davies AM,Greenwood C,Mauk AG,Smith M,Williamson DJ,Moore GR

更新日期：1991-06-24 00:00:00