Evidence that oxidative dephosphorylation by the nonheme Fe(II), α-ketoglutarate:UMP oxygenase occurs by stereospecific hydroxylation.

abstract：:Electron paramagnetic resonance (EPR) spectroscopy and analysis of the primary structure of the CMP-N-acetylneuraminic acid hydroxylase revealed that this enzyme is the first iron-sulphur protein of the Rieske type to be found in the cytosol of Eukarya. The dithionite-reduced hydroxylase exhibited an EPR signal known ...

journal_title：FEBS letters

authors： Schlenzka W,Shaw L,Kelm S,Schmidt CL,Bill E,Trautwein AX,Lottspeich F,Schauer R

更新日期：1996-05-06 00:00:00

abstract：:The 3-nitrosobenzamide (NOBA) drug abolishes SIV replication sharply at 20 microM concentration when CEM x 174 cells are preincubated for 1 h with the drug prior to viral infection. Treatment of CEM x 174 cells with 20 microM NOBA resulted in the inhibition of the synthesis of the DNA sequence coding for the gag gene,...

journal_title：FEBS letters

authors： Chuang AJ,Killam KF Jr,Chuang RY,Rice WG,Schaeffer CA,Mendeleyev J,Kun E

更新日期：1993-07-12 00:00:00

abstract：:Sensitivity of mammalian cells to the bacterial toxin aerolysin is due to the presence at their surface of glycosylphosphatidyl inositol (GPI)-anchored proteins which act as receptors. Using a panel of mutants that are affected in the GPI biosynthetic pathway and Trypanosoma brucei variant surface glycoproteins, we sh...

journal_title：FEBS letters

authors： Abrami L,Velluz MC,Hong Y,Ohishi K,Mehlert A,Ferguson M,Kinoshita T,Gisou van der Goot F

更新日期：2002-02-13 00:00:00

abstract：:The tuf gene of eubacteria, encoding the EF-tu elongation factor, was duplicated early in the evolution of the taxon. Phylogenetic and genomic location analysis of 20 complete eubacterial genomes suggests that this ancient duplication has been differentially lost and maintained in eubacteria. ...

journal_title：FEBS letters

authors： Lathe WC 3rd,Bork P

更新日期：2001-08-03 00:00:00

abstract：:The gene for human tetranectin was isolated from a genomic library with a mixture of degenerate oligonucleotide probes. The gene is about 12 kbp and contains two intervening sequences. The gene encodes a protein of 202 amino acid residues, with a signal peptide of 21 amino acid residues, followed by the tetranectin se...

journal_title：FEBS letters

authors： Berglund L,Petersen TE

更新日期：1992-08-31 00:00:00

abstract：:DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including ...

journal_title：FEBS letters

authors： Jingami H,Mizuno N,Takahashi H,Shibahara S,Furutani Y,Imura H,Numa S

更新日期：1985-10-21 00:00:00

abstract：:Pathway analysis methods are frequently applied to cancer gene expression data to identify dysregulated pathways. These methods often infer pathway activity based on the expression of genes belonging to a given pathway, even though the proteins ultimately determine the activity of a given pathway. Furthermore, the ass...

journal_title：FEBS letters

authors： Szalai B,Saez-Rodriguez J

更新日期：2020-12-01 00:00:00

abstract：:Different hydrophobic glycoproteins are associated to native biliary vesicles, which are the major carrier of biliary cholesterol. Some of these proteins promote cholesterol crystallization, a key step in cholesterol gallstone formation. This study was specifically conducted to identify the 130 kDa biliary vesicle-ass...

journal_title：FEBS letters

authors： Núñez L,Amigo L,Rigotti A,Puglielli L,Mingrone G,Greco AV,Nervi F

更新日期：1993-08-23 00:00:00

abstract：:Osteogenesis depends on a coordinated network of transcription factors including Sp7. Emerging evidence indicates that microRNAs (miRNAs) act as pivotal regulators in various biological processes including osteoblast proliferation and differentiation. Here, we investigated the effect of miR-145 on osteogenic different...

journal_title：FEBS letters

authors： Jia J,Tian Q,Ling S,Liu Y,Yang S,Shao Z

更新日期：2013-09-17 00:00:00

abstract：:The interaction of Bordetella pertussis adenylate cyclase toxin (CyaA) with complement receptor 3 (CR3, CD11b/CD18) involves N-linked oligosaccharide chains. To investigate the relative importance of the individual N-glycans of CR3 for toxin activity, the asparagine residues of the consensus N-glycosylation sites of C...

journal_title：FEBS letters

authors： Hasan S,Osickova A,Bumba L,Novák P,Sebo P,Osicka R

更新日期：2015-01-30 00:00:00

abstract：:The Golgi apparatus is known to modify and sort newly synthesized secretory proteins. However, fundamental mysteries remain about the structure, operation, and dynamics of this organelle. Important insights have emerged from studying the Golgi in yeasts. For example, yeasts have provided direct evidence for Golgi cist...

journal_title：FEBS letters

authors： Papanikou E,Glick BS

更新日期：2009-12-03 00:00:00

abstract：:We have used a competitive enzyme-linked immunoassay with a panel of monoclonal antibodies to probe the topography of the membrane-bound form of apolipoprotein B (apo B) in rabbit microsomes. All epitopes investigated were found to be expressed at the cytosolic side of the microsomal membrane under conditions in which...

journal_title：FEBS letters

authors： Wilkinson J,Higgins JA,Groot PH,Gherardi E,Bowyer DE

更新日期：1992-06-08 00:00:00

abstract：:Higher basal 2-deoxy-D-glucose uptake in rheumatoid synovial cells than in non-rheumatoid synovial cells, was found to be associated with an increased interleukin-1 beta (IL-1 beta) secretion (respectively 850 +/- 238 vs. 8.3 +/- 2.4 pg/24 h/10(5) cells, mean +/- S.E.M.). When exogenous human recombinant IL-1 beta was...

journal_title：FEBS letters

authors： Hernvann A,Aussel C,Cynober L,Moatti N,Ekindjian OG

更新日期：1992-05-25 00:00:00

abstract：:A cytosolic calmodulin wave has been described in the pre-replicative phase of rat liver regeneration. Here we demonstrate that this calmodulin increase is reduced by injection of cycloheximide (translation inhibitor) and actinomycin D (transcription inhibitor). In addition, we found that calmodulin mRNA levels are in...

journal_title：FEBS letters

authors： Piõl MR,Berchtold MW,Bachs O,Heizmann CW

更新日期：1988-04-25 00:00:00

abstract：:Treatment of human vascular smooth muscle cells (SMC) with human alpha-thrombin greatly increased DNA synthesis and cell proliferation. Both the integrity of the catalytic site and that of the anion binding exosite were required for expression of this activity. Experiments employing Northerns indicated induction of c-...

journal_title：FEBS letters

authors： Kanthou C,Parry G,Wijelath E,Kakkar VV,Demoliou-Mason C

更新日期：1992-12-14 00:00:00

abstract：:This study describes the comparative analysis of two insect recombinant aminotransferases, Aedes aegypti 3-hydroxykynurenine (3-HK) transaminase/alanine glyoxylate aminotransferase (Ae-HKT/AGT) and Drosophila melanogaster serine pyruvate aminotransferase (Dm-Spat), which share 52% identity in their amino acid sequence...

journal_title：FEBS letters

authors： Han Q,Li J

更新日期：2002-09-11 00:00:00

abstract：:The ARG-11 gene in Saccharomyces cerevisiae encodes a protein with the characteristic features of a family of 35 related membrane proteins that are encoded in the fungal genome. Some of them are known to transport various substrates and products across the inner membranes of mitochondria, but the functions of 29 membe...

journal_title：FEBS letters

authors： Palmieri L,De Marco V,Iacobazzi V,Palmieri F,Runswick MJ,Walker JE

更新日期：1997-06-30 00:00:00

abstract：:Osteoblasts and adipocytes are believed to share a common progenitor. Peroxisome proliferator-activated receptor gamma (PPARgamma) plays a key role in the switching of these two cell lineages. Here, we demonstrated the differentiation of ES cells into an osteoblastic lineage using siRNA against PPARgamma without the a...

journal_title：FEBS letters

authors： Yamashita A,Takada T,Nemoto K,Yamamoto G,Torii R

更新日期：2006-07-24 00:00:00

abstract：:'Peripheral' binding sites for benzodiazepines are under neural or homonal control in the pineal gland, olfactory bulb, and kidney. These observations prompted a search for an endogenous substance which could modulate these sites under physiological conditions. Acidified methanol extracts from several tissues (e.g. st...

journal_title：FEBS letters

authors： Mantione CR,Weissman BA,Goldman ME,Paul SM,Skolnick P

更新日期：1984-10-15 00:00:00

abstract：:We demonstrated that unmodified and modified (phosphorothioate) oligonucleotides prevent cDNA synthesis by AMV or HIV reverse transcriptases. Antisense oligonucleotide/RNA hybrids specifically arrest primer extension. The blockage involves the degradation of the RNA fragment bound to the antisense oligonucleotide by t...

journal_title：FEBS letters

authors： Hatta T,Kim SG,Nakashima H,Yamamoto N,Sakamoto K,Yokoyama S,Takaku H

更新日期：1993-09-13 00:00:00

abstract：:A covalent complex formed by bacterial tRNAs and prothymosin alpha, an abundant acidic nuclear protein involved in proliferation of mammalian cells, upon production of the recombinant rat protein in Escherichia coli cells was studied. Several tRNA attachment sites were identified in the prothymosin alpha molecule usin...

journal_title：FEBS letters

authors： Lukashev DE,Chichkova NV,Vartapetian AB

更新日期：1999-05-21 00:00:00

abstract：:The characteristics of renal transport of L-alanine by luminal-membrane vesicles from proximal straight tubules (pars recta) of rabbit kidney were investigated. The following picture emerges from transport studies. Two electrogenic and Na+ requiring systems confined to this region of the nephron exist for the transpor...

journal_title：FEBS letters

authors： Vorum H,Jessen H,Jørgensen KE,Sheikh MI

更新日期：1988-01-18 00:00:00

abstract：:Metal-binding peptides in proteolytic digest maps have been identified by matrix-assisted UV laser desorption time-of-flight mass spectrometry (LDTOF-MS). The plasma and milk metal transport protein chosen to demonstrate this process, histidine-rich glycoprotein (HRG), was purified and then digested with trypsin; the ...

journal_title：FEBS letters

authors： Hutchens TW,Nelson RW,Yip TT

更新日期：1992-01-13 00:00:00

abstract：:In thrombin-stimulated platelets alpha IIb beta 3 integrin engagement triggers both phosphatidylinositol 3',4'-bisphosphate synthesis and calpain activation. We checked the possible involvement of calpains in phosphatidylinositol 3-kinase signalling pathway using a cell permeant specific inhibitor of calpains, calpept...

journal_title：FEBS letters

authors： Montsarrat N,Racaud-Sultan C,Mauco G,Plantavid M,Payrastre B,Breton-Douillon M,Chap H

更新日期：1997-03-03 00:00:00

abstract：:Using specific antisera, the expression of the G protein alpha subunits, G8, G(i1), G(i2), G(i3) and G0, were determined in 3T3-F442A cells during their differentiation to adipocytes in a hormonally defined medium. Differentiation caused distinct increases in the expression of two Gs isoforms and decreases in the expr...

journal_title：FEBS letters

authors： Kilgour E,Anderson NG

更新日期：1993-08-16 00:00:00

abstract：:Oxygen is an essential element in the survival of complex organisms, however the level of oxygen, low or high, can be a source of stress depending on the biological context. Low levels of oxygen in tissues (hypoxia) can be the consequence of a number of pathophysiological conditions including ischemic disorders and ca...

journal_title：FEBS letters

authors： Brahimi-Horn MC,Pouysségur J

更新日期：2007-07-31 00:00:00

abstract：:Buthionine sulfoximine (BSO) induces decreased glutathione (GSH) and trypanothione [T(SH)2 ] pools in trypanosomatids, presumably because only gamma-glutamylcysteine synthetase (γECS) is blocked. However, some BSO effects cannot be explained by exclusive γECS inhibition; therefore, its effect on the T(SH)2 metabolism ...

journal_title：FEBS letters

authors： Vázquez C,Mejia-Tlachi M,González-Chávez Z,Silva A,Rodríguez-Zavala JS,Moreno-Sánchez R,Saavedra E

更新日期：2017-12-01 00:00:00

abstract：:Glycogen phosphorylase b at concentrations close to those found in skeletal muscle interacts with sarcoplasmic reticulum membranes, but not with liposomes made of lipids extracted from these membranes, and is inhibited upon binding to the membrane. The interaction of glycogen phosphorylase with the sarcoplasmic reticu...

journal_title：FEBS letters

authors： Cuenda A,Centeno F,Gutierrez-Merino C

更新日期：1991-06-03 00:00:00

abstract：:The inositol (1,4,5)-trisphosphate receptor (InsP3R) is an intracellular calcium release channel that plays a crucial role in cell signaling. In Drosophila melanogaster, a single InsP3R gene (itpr) encodes a protein (DmInsP3R) that is approximately 60% conserved with mammalian InsP3Rs. The functional properties of wil...

journal_title：FEBS letters

authors： Srikanth S,Wang Z,Hasan G,Bezprozvanny I

更新日期：2004-09-24 00:00:00

abstract：:Ballistic transfection, based on cell and tissue bombardment by the tungsten and gold microparticles covered with the gene DNA, was used for the delivery of a bacterial beta-galactosidase and a full-length cDNA copy of the human dystrophin genes into mouse skeletal muscles. CMV-lacZ, SV40-lacZ, LTR-lacZneo and full-le...

journal_title：FEBS letters

authors： Zelenin AV,Kolesnikov VA,Tarasenko OA,Shafei RA,Zelenina IA,Mikhailov VV,Semenova ML,Kovalenko DV,Artemyeva OV,Ivaschenko TE,Evgrafov OV,Dickson G,Baranovand VS

更新日期：1997-09-08 00:00:00