Abstract:
:A 122 nt uridine-rich sequence (URE) in the Na(+)/glucose cotransporter (SGLT1) mRNA 3'-untranslated region is critical for cAMP-dependent message stabilization. Its function was investigated in LLC-PK(1) cells stably expressing beta-globin reporter transcripts. Insertion of the SGLT1 URE downstream from an unrelated destabilizing sequence, the c-fos ARE, evoked cAMP-dependent message stabilization. Stabilization was blocked by a substitution mutation within the SLGT1 URE. These observations indicate that the SGLT1 URE is sufficient to transmit cAMP-dependent, cis-dominant mRNA stabilization in the presence of appropriate trans-acting factors and appears to function independently of the nature of the destabilizing domain.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Loflin P,Lever JEdoi
10.1016/s0014-5793(01)02260-8keywords:
subject
Has Abstractpub_date
2001-03-16 00:00:00pages
233-7issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02260-8journal_volume
492pub_type
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