Abstract:
:Two hexokinases were characterized in Schizosaccharomyces pombe: hexokinase 1, with a low phosphorylation coefficient on glucose (Km 8.5 mM) and hexokinase 2, a kinetically conventional hexokinase. Genes hxk1+ and hxk2+ encoding these enzymes were cloned and sequenced. Disruption of hxk1+ had no effect on growth but disruption of hxk2+ doubled the generation time in glucose. Spores carrying the double disruption hxk1+ hxk2+ did not grow on glucose or fructose after one week. Expression of hxk1+ increased strongly during growth in fructose or glycerol. Expression of hxk2+ was highest during growth in glycerol. A NADP-dependent glucose dehydrogenase was detected, but not a glucokinase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Petit T,Blázquez MA,Gancedo Cdoi
10.1016/0014-5793(95)01451-9subject
Has Abstractpub_date
1996-01-08 00:00:00pages
185-9issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(95)01451-9journal_volume
378pub_type
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