Abstract:
:Translocation of folded proteins by the Tat system of Escherichia coli is believed to rely on the presence of phosphatidylethanolamine (PE) and the negatively charged phospholipids cardiolipin (CL) and phosphatidylglycerol (PG). Here, we show that while PE is indeed essential for activity, the Tat system is fully functional in a clsA/clsB/clsC deletion strain lacking CL, and in a pgsA deletion strain lacking both PG and CL during aerobic growth on complex media. In contrast to early studies that relied on strains with reduced lipid levels, this study therefore demonstrates that PG and CL are dispensable for Tat transport. The lack of these lipids may be compensated by other anionic phospholipids such as phosphatidic acid, CDP-diacylglycerol or N-acyl-PE.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rathmann C,Schlösser AS,Schiller J,Bogdanov M,Brüser Tdoi
10.1002/1873-3468.12794subject
Has Abstractpub_date
2017-09-01 00:00:00pages
2848-2858issue
18eissn
0014-5793issn
1873-3468journal_volume
591pub_type
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