Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support.

abstract：:A cDNA of a type 7 adenylyl cyclase isoform was cloned from a bovine retinal pigment epithelium cDNA library using oligonucleotides developed to conserved regions common to mammalian adenylyl cyclases. A 6.7 kb mRNA of very high abundance was uniquely present on Northern blots containing mRNA or total RNA from the pig...

journal_title：FEBS letters

authors： Völkel H,Beitz E,Klumpp S,Schultz JE

更新日期：1996-01-15 00:00:00

abstract：:The residues on MAP kinase kinase-1 (MAPKK1) phosphorylated by MAP kinase in vitro have been identified as Thr-291 and Thr-385. Both threonines are phosphorylated in PC12 cells and the 32P-labelling of each residue increases after stimulation with nerve growth factor (NGF). The results establish that MAPKK1 is a physi...

journal_title：FEBS letters

authors： Saito Y,Gomez N,Campbell DG,Ashworth A,Marshall CJ,Cohen P

更新日期：1994-03-14 00:00:00

abstract：:The influence of aniso-osmolarity on the activity of the MAP kinases Erk-1 and Erk-2 was studied in C6 glioma cells. Hypo-osmotic treatment (205 mosmol/l) led to an increased activity of Erk-1 and Erk-2 within 3 min, which became maximal at 10 min and returned to basal level within 120 min. In contrast, Erk activity w...

journal_title：FEBS letters

authors： Sinning R,Schliess F,Kubitz R,Häussinger D

更新日期：1997-01-03 00:00:00

abstract：:Using antibody against the Rho binding domain of ROKalpha, two neuronal phosphoproteins of 62 and 80 kDa were co-immunoprecipitated from brain extracts. Peptide analysis revealed their identity as collapsin response mediator proteins (CRMPs); p62 was CRMP-2 whereas p80 was a novel splice form of CRMP-1 with an extende...

journal_title：FEBS letters

authors： Leung T,Ng Y,Cheong A,Ng CH,Tan I,Hall C,Lim L

更新日期：2002-12-18 00:00:00

abstract：:A lowered efficiency of oxidative phosphorylation was recently found in a Leber hereditary optic neuropathy (LHON) proband carrying a mutation in the mtDNA gene for subunit 6 of the membrane-bound F0 segment of the F1F0-ATP synthase [9]. This phenotype was transferred to cytoplasmic hybrid cells together with the muta...

journal_title：FEBS letters

authors： Majander A,Lamminen T,Juvonen V,Aula P,Nikoskelainen E,Savontaus ML,Wikström M

更新日期：1997-07-28 00:00:00

abstract：:Barley thioredoxin h isozymes 1 (HvTrxh1) and barley thioredoxin h isozymes 2 (HvTrxh2) show distinct spatiotemporal distribution in germinating seeds. Using a novel approach involving measurement of bidirectional electron transfer rates between Escherichia coli thioredoxin, which exhibits redox-dependent fluorescence...

journal_title：FEBS letters

authors： Maeda K,Hägglund P,Björnberg O,Winther JR,Svensson B

更新日期：2010-08-04 00:00:00

abstract：:The geometries are reported for interacting arginine-carboxyl pairs obtained from 37 high resolution protein structures solved to a resolution of 2.0 A or better. The closest interatomic distance between the guanidinium and carboxyl is less than 4.2 A for 74 arginine and carboxyl groups, with the majority of these lyi...

journal_title：FEBS letters

authors： Singh J,Thornton JM,Snarey M,Campbell SF

更新日期：1987-11-16 00:00:00

abstract：:A novel GTP binding protein (G protein) alpha subunit cDNA was isolated from a T cell leukemia cell line, Jurkat, utilizing polymerase chain reaction (PCR). The predicted amino acid sequence of this G protein alpha subunit showed the highest identity (96.6%) to bovine cone cell-specific transducin (Tc alpha). The orga...

journal_title：FEBS letters

authors： Kubo M,Hirano T,Kakinuma M

更新日期：1991-10-21 00:00:00

abstract：:Furin, a member of the subtilisin-like pro-protein convertase family, is a type I membrane protein that undergoes ectodomain shedding. Metabolic labeling of cells stably expressing furin demonstrated that the shed form of furin is detected after 30 min. Moreover, sequence analysis revealed that specific residues of th...

journal_title：FEBS letters

authors： Denault J,Bissonnette L,Longpré J,Charest G,Lavigne P,Leduc R

更新日期：2002-09-11 00:00:00

abstract：:Dinucleotide polyphosphates (ApnA) have emerged as signaling molecules in rapidly dividing cells. The presence and role of Ap5A in the heart remain unknown. Here, we report that the myocardium contains abundant amounts of diadenosine 5',5"-P1,P5-pentaphosphate (Ap5A), a member of the ApnA family. Ischemia induced 10-f...

journal_title：FEBS letters

authors： Jovanovic A,Jovanovic S,Mays DC,Lipsky JJ,Terzic A

更新日期：1998-02-27 00:00:00

abstract：:Methanosarcina barkeri inserts pyrrolysine (Pyl) at an in-frame UAG codon in its monomethylamine methyltransferase gene. Pyrrolysyl-tRNA synthetase acylates Pyl onto tRNAPyl, the amber suppressor pyrrolysine Pyl tRNA. Here we show that M. barkeri Fusaro tRNAPyl can be misacylated with serine by the M. barkeri bacteria...

journal_title：FEBS letters

authors： Gundllapalli S,Ambrogelly A,Umehara T,Li D,Polycarpo C,Söll D

更新日期：2008-10-15 00:00:00

abstract：:Stimulation of the bicarbonate dehydration reaction in thylakoid suspension under conditions of saturating light at pH 7.6-8.0 was discovered. This effect was inhibited by nigericin or the lipophilic carbonic anhydrase (CA) inhibitor ethoxyzolamide (EZ), but not by the hydrophilic CA inhibitor, acetazolamide. It was s...

journal_title：FEBS letters

authors： Moskvin OV,Ivanov BN,Ignatova LK,Kollmeier MA

更新日期：2000-03-31 00:00:00

abstract：:The cDNA coding for mature human alpha 1-proteinase inhibitor (alpha 1-PI) has been inserted into a variety of yeast expression vectors. Yeast cells transformed with these plasmids were then assayed for the production of mature, unglycosylated alpha 1-PI. The production level is optimal when the recombinant plasmid ca...

journal_title：FEBS letters

authors： Hoylaerts M,Weyens A,Bollen A,Harford N,Cabezón T

更新日期：1986-08-11 00:00:00

abstract：:In the present study we show the existence of a functional nitric oxide synthase (NOS) in rat liver mitochondria. The enzyme uses L-arginine (L-arg) to produce nitric oxide (NO) and L-citrulline, and is Ca2+-dependent. L-Arg analogues, N(omega)monomethyl-L-arg and N(omega)-nitro-L-arg, inhibit the enzyme, and D-argini...

journal_title：FEBS letters

authors： Ghafourifar P,Richter C

更新日期：1997-12-01 00:00:00

abstract：:The effects of mildly acidic conditions on the free energy of unfolding (DeltaG(u)(buff)) of the pore-forming alpha-hemolysin (alphaHL) from Staphylococcus aureus were assessed between pH 5.0 and 7.5 by measuring intrinsic tryptophan fluorescence, circular dichroism and elution time in size exclusion chromatography du...

journal_title：FEBS letters

authors： Bortoleto RK,Ward RJ

更新日期：1999-10-15 00:00:00

abstract：:Mesenchymal stem cells (MSC) transplantation has been shown to decrease fibrosis in the heart; however, whether MSC directly influence the function of cardiac fibroblasts (CFB) remains unknown. MSC-conditioned medium significantly attenuated proliferation of CFB compared with CFB-conditioned medium. MSC-conditioned me...

journal_title：FEBS letters

authors： Ohnishi S,Sumiyoshi H,Kitamura S,Nagaya N

更新日期：2007-08-21 00:00:00

abstract：:Physiological and pathological states that are associated with elevated plasma fatty acids (FAs) increase uncoupling protein 2 (UCP2) mRNA in white adipose tissue and UCP3 mRNA in skeletal muscle and heart. A direct effect of unsaturated fatty acids from all classes has been shown in various cultured cells. There is e...

journal_title：FEBS letters

authors： Thompson MP,Kim D

更新日期：2004-06-18 00:00:00

abstract：:The expression of the cytochrome P4501A1 gene, CYP1A1, is induced by e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) mainly by transcriptional mechanisms. The inducers mediate their effect upon binding and activation of the aryl hydrocarbon receptor (AHR) transcription-factor complex. Utilizing chimeric CYP1A1/CAT con...

journal_title：FEBS letters

authors： Jørgensen EC,Autrup H

更新日期：1995-05-29 00:00:00

abstract：:We present the three-dimensional structure of the N-terminal FK506-binding protein (FKBP)-like domain of the immunophilin FKBP42 from Arabidopsis thaliana. The data provide the structural background for the explanation of key functional properties reported previously. ...

journal_title：FEBS letters

authors： Weiergräber OH,Eckhoff A,Granzin J

更新日期：2006-01-09 00:00:00

abstract：:A simple model is put forward to explain the long-known three-base periodicity in coding DNA. We propose the concept of same-phase triplet clustering, i.e. a condition wherein a triplet appears several times in one phase without interruption by the two other possible phases. For instance, in the sequence (i): NTT_GNN_...

journal_title：FEBS letters

authors： Sánchez J,López-Villaseñor I

更新日期：2006-11-27 00:00:00

abstract：:The chloroplast inner envelope translocon subunit Tic110 is imported via a soluble stromal translocation intermediate. In this study an in-organellar import system is established which allows for an accumulation of this intermediate in order to analyze its requirements for reexport. All results demonstrate that the re...

journal_title：FEBS letters

authors： Vojta L,Soll J,Bölter B

更新日期：2007-06-12 00:00:00

abstract：:Chemical conjugation of fatty acids to antibodies generates lipid-modified molecules which have found use in the targeting of liposome-mediated drug delivery and in liposome-based immunoassays. Alternatively, bacterial expression of antibodies as single-chain FV fragments fused to lipoprotein signal peptide and N-term...

journal_title：FEBS letters

authors： Keinänen K,Laukkanen ML

更新日期：1994-06-06 00:00:00

abstract：:C6 rat glioma cells persistently infected with subacute sclerosing panencephalitis virus (C6/SSPE) were treated with measles antiserum and purified anti-measles IgG. This stimulated phosphoinositide breakdown and an increase in inositol phosphates. In uninfected C6 cells, however, only fetal calf serum (FCS), but not ...

journal_title：FEBS letters

authors： Weinmann-Dorsch C,Koschel K

更新日期：1989-04-24 00:00:00

abstract：:In this work, we have spectroscopically characterised CYP157C1 from Streptomyces coelicolor A3(2) which has the motif E(297)QSLW(301) rather than the invariant EXXR motif in the P450 K-helix. Site-directed mutagenesis of native E(297)QSLW(301) in CYP157C1 to E(297)ESLR(301) or E(297)QSRW(301) both containing standard ...

journal_title：FEBS letters

authors： Rupasinghe S,Schuler MA,Kagawa N,Yuan H,Lei L,Zhao B,Kelly SL,Waterman MR,Lamb DC

更新日期：2006-11-27 00:00:00

abstract：:HMr alpha 2CPI was found to be an inhibitor of human liver cathepsin H by the measurement of the dissociation constant (Ki), the association rate constant (k1) and the dissociation rate constant (k-1) between the enzyme and the inhibitor. These data suggest that this protein-proteinase inhibitor can play a physiologic...

journal_title：FEBS letters

authors： Pagano M,Engler R

更新日期：1984-01-23 00:00:00

abstract：:The aim of this study was to investigate the distribution of a calcium-inhibitable adenylyl cyclase type VI (type VI AC) in the central nervous system using an antiserum directed against the N-terminus of type VI AC. Our results indicate that type VI AC immunoreactivity is generally expressed in many brain regions wit...

journal_title：FEBS letters

authors： Liu FC,Wu GC,Hsieh ST,Lai HL,Wang HF,Wang TW,Chern Y

更新日期：1998-09-25 00:00:00

abstract：:Using the method of contextual analysis, we have studied the collection of somatic mutations in immunoglobulin genes. It has been found that the emergence of somatic mutations can be based on the reparation of complementarity violations in the heteroduplexes corresponding to complementary palindromes or direct repeats...

journal_title：FEBS letters

authors： Kolchanov NA,Solovyov VV,Rogozin IB

更新日期：1987-04-06 00:00:00

abstract：:Linkage between the high-capacity Ca2+-binding protein calsequestrin and the ryanodine receptor is proposed to be essential for proper Ca2+-release during skeletal muscle excitation-contraction coupling. However, no direct biochemical evidence exists showing a connection between these high-molecular-mass complexes in ...

journal_title：FEBS letters

authors： Murray BE,Ohlendieck K

更新日期：1998-06-16 00:00:00

abstract：:The OxyR protein is a transcriptional activator for a subset of peroxide stress-inducible genes, most of which are involved in defense systems against oxidative stress. Recently, it was demonstrated that purified OxyR has one intramolecular disulfide bond, which led to the proposal that the reversible disulfide bond f...

journal_title：FEBS letters

authors： Tao K

更新日期：1999-08-20 00:00:00

abstract：:Three regions of the alpha chain of Torpedo californica acetylcholine receptor (AChR), corresponding to residues alpha 262-276, alpha 388, 408 and alpha 427-437 were synthesized, purified and characterized. The first two peptides have been proposed to occupy inter-transmembrane regions while the third represented the ...

journal_title：FEBS letters

authors： Atassi MZ,Manshouri T,Yokoi T

更新日期：1988-02-15 00:00:00