Abstract:
:Various cDNAs coding for part or all of human pre-pro lactase-phlorizin hydrolase (pre-proLPH) were transfected into COS cells and the subcellular location of the lactase-related proteins assessed. Only the complete proLPH reached the plasma membrane. LPH without the pro sequence, and a construct containing the pro sequence and the lactase domain of mature LPH, accumulated intracellularly; the pro sequence with no mature domain was secreted. We conclude that the pro sequence is important for LPH to be transported to the cell surface.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Oberholzer T,Mantei N,Semenza Gdoi
10.1016/0014-5793(93)80389-csubject
Has Abstractpub_date
1993-10-25 00:00:00pages
127-31issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(93)80389-Cjournal_volume
333pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::In mammalian cells, the Bcl-2 and Bcl-x(L) proteins suppress programmed cell death whereas the topographically similar Bax protein accelerates the apoptotic process. Recently published data suggest that expression of the human Bax-alpha gene is lethal for the yeast Saccharomyces cerevisiae and that this toxicity can b...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00044-0
更新日期:1996-02-12 00:00:00
abstract::The corpus of the scientific literature has reached such size that a lot of useful data, dispersed throughout millions different articles, are now hard to recover. For instance, many articles in the biological domain describe relationships between entities (gene, proteins, small molecules, etc.) yet this crucial infor...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2008.02.071
更新日期:2008-04-09 00:00:00
abstract::The BChla-containing B880-complex (core-complex) of Rhodopseudomonas marina (Rhodospirillaceae) was isolated with a new purification method. The isolation of the B880-complex was performed by solubilisation of the photosynthetic membranes with the detergent LDAO and subsequent fractionated ammonium-sulfate precipitati...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81383-w
更新日期:1992-10-19 00:00:00
abstract::We used cDNA microarray to identify transforming growth factor beta (TGF-beta) responsive target genes during osteoblast development and found that nephronectin (Npnt) is one such gene that is significantly down-regulated. Here we report the role of TGF-beta in regulating Npnt-mediated osteoblast differentiation. We f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.04.074
更新日期:2010-07-02 00:00:00
abstract::Cultured rat hepatocytes exhibit acid phospholipase A activity. On the basis of product formation from stereospecifically radiolabeled phosphatidylethanolamine substrates, phospholipases A1 and A2 have been identified with optimal activities at pH 4.5. According to subcellular fractionation studies, the acid phospholi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80062-0
更新日期:1988-08-29 00:00:00
abstract::Granulocytes from human eosinophilic donors were incubated with arachidonic acid or 15-hydroxyeicosatetraenoic acid (15-HETE) and stimulated with the ionophore A23187. The eicosanoids were extracted with reversed-phase cartridges and subjected to RP-HPLC analysis. When extracts from eosinophil-enriched populations wer...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81078-6
更新日期:1989-09-11 00:00:00
abstract::Adhesion of platelets to the exposed extracellular matrix proteins at sites of vascular injury is partly regulated by the local fluid shear stress. Because the Leu33Pro (Pl(A)) polymorphism of integrin beta(3) confers only a modest increase in adhesion under static conditions, we used CHO and 293 cells expressing the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00170-4
更新日期:2003-04-10 00:00:00
abstract::While negative effect of smoking on the resistance to viral infections was known, the underlying mechanisms remained unclear. Here we report that products of cigarette smoking compromise the cellular anti-viral defenses by inhibiting the signaling induced by Type I interferon (IFN). Cigarette smoking condensate (but n...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.08.013
更新日期:2008-09-22 00:00:00
abstract::A simple method was developed for the isolation of primase-free DNA polymerase-alpha from the DNA polymerase-alpha-primase complex of mouse FM3A cells. The polymerase was separated from primase subunits by chromatography on a single-stranded DNA-cellulose column in the presence of 50% etylene glycol. The primase-free ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81043-n
更新日期:1990-10-29 00:00:00
abstract::Genetic factors seem to play a significant role in susceptibility to systemic lupus erythematosus (SLE). We previously described the amino acid polymorphism (Val14Met) within the IFN-gamma receptor 1 (IFN-gammaRI), and that the frequency of the Metl4 allele in SLE patients was significantly higher than that of the hea...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00701-2
更新日期:1999-06-18 00:00:00
abstract::H2O2 addition to the oxidized cytochrome c oxidase reconstituted in liposomes brings about a red shift of the Soret band of the enzyme and an increased absorption in the visible region with two distinct peaks at approximately 570 and 605 nm. Throughout pH range 6-8.5, the spectral changes at 570 nm and in the Soret ba...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80258-2
更新日期:1987-07-27 00:00:00
abstract::Using the patch-clamp technique, we demonstrate here the opening of K+ channels evoked by the actively transported amino acid L-alanine in isolated Necturus enterocytes. These channels had a conductance of about 30 pS and their activation was dependent on transmembrane electrical potential and cytosolic Ca2+. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80134-0
更新日期:1988-07-18 00:00:00
abstract::Little is known about the mechanisms underlying calcium-induced Ca2+ release (CICR) in plants. The slow-activating vacuolar (SV) channel is both permeable to, and activated by Ca2+, and is therefore a prime candidate for a role in CICR. Cytosol-side-out vacuolar membrane vesicles loaded with 45Ca2+ showed voltage- and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01109-6
更新日期:1999-09-10 00:00:00
abstract::Gastrin (G17) and N-carboxymethylgastrin (G17-Gly) have been shown to stimulate the growth of colon cancer cells both in vivo and in vitro. The identity of the receptor mediating these effects is controversial. A recent study demonstrated the presence of a low affinity binding site for G17 and G17-Gly on the DLD-1 hum...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01408-x
更新日期:2004-01-02 00:00:00
abstract::We describe the identification and characterization of the BMH1 gene from the yeast Saccharomyces cerevisiae. The gene encodes a putative protein of 292 amino acids which is more than 50% identical with the bovine brain 14-3-3 protein and proteins isolated from sheep brain which are strong inhibitors of protein kinase...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80426-h
更新日期:1992-05-11 00:00:00
abstract::PMA-induced leukotriene C4 synthase (LTC4S) phosphorylation was investigated over a period of 8 h in a monocytic cell line (THP-1). The level of LTC4S phosphorylation was increased 3-5 fold over a 4 h period decreasing to basal levels after 8 h. This phosphorylation event was found to be specific to THP-1 cells as the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00397-x
更新日期:1999-04-16 00:00:00
abstract::The early response to bacterial inoculation has been investigated and two Arabidopsis genes, ap3.3a and ap4.3a have been characterized. The AP3.3A protein showed high identity to centrin, a ubiquitous cytoskeletal protein first identified in unicellular green alga. Amino-acid sequence analyses of the AP4.3A protein in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01015-1
更新日期:1998-09-04 00:00:00
abstract::Partially reduced metabolites of molecular oxygen, superoxide (O2-) and hydrogen peroxide (H2O2), are detected in respiratory tract lining fluid, and it is assumed that these are key components of innate immunity. Whether these reactive oxygen species (ROS) are produced specifically by the respiratory epithelium in re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.08.002
更新日期:2005-08-29 00:00:00
abstract::Beta-thymosins sequester G-actin and preserve a pool of monomers of actin which constitute an important prerequisite for cellular function of the microfilament system. To study the influence of paraquat binding to G-actin on the interaction of G-actin with thymosin beta4 we determined the apparent dissociation constan...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00295-6
更新日期:1998-04-03 00:00:00
abstract::To identify the linear epitope for Fc-binding on the bovine IgG2 Fc receptor (boFcgamma2R), peptides derived from the membrane-distal extracellular domain (EC1) of boFcgamma2R corresponding to the homologous region of human FcalphaRI were synthesized. Binding of bovine IgG2 to the different peptides was tested by Dot-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.01.060
更新日期:2006-02-20 00:00:00
abstract::So far, various approaches for phylogenetic analysis have been developed. Almost all of them put stress on analyzing nucleic acid sequences or protein primary structures. In this paper, we take the physicochemical properties of amino acids into account and introduce the hydropathy profile of amino acids into phylogene...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.086
更新日期:2006-10-02 00:00:00
abstract::F(0)F(1)-ATP synthase couples ATP synthesis/hydrolysis with transmembrane proton transport. The catalytic mechanism involves rotation of the gamma epsilon c(approximately 10)-subunits complex relative to the rest of the enzyme. In the absence of protonmotive force the enzyme is inactivated by the tight binding of MgAD...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.08.030
更新日期:2005-09-26 00:00:00
abstract::Methanosarcina barkeri inserts pyrrolysine (Pyl) at an in-frame UAG codon in its monomethylamine methyltransferase gene. Pyrrolysyl-tRNA synthetase acylates Pyl onto tRNAPyl, the amber suppressor pyrrolysine Pyl tRNA. Here we show that M. barkeri Fusaro tRNAPyl can be misacylated with serine by the M. barkeri bacteria...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.08.027
更新日期:2008-10-15 00:00:00
abstract::VirG, PhoB, and OmpR are positive regulators of the virulence, phosphate, and osmolarity regulons, respectively. Their target genes are attended with one or more significant blocks of similar 6-bp sequences in their regulatory regions. The distance between the block(s) and transcriptional start site varies with the ta...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(90)80691-b
更新日期:1990-04-09 00:00:00
abstract::The Na-Ca exchanger is essential for the Ca2+ homeostasis in many cell types. This transporter has been difficult to investigate because no specific inhibitor is available. We have synthesized an antisense oligodeoxynucleotide directed against the rat cardiac Na-Ca exchanger mRNA. To estimate the activity of the Na-Ca...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00391-l
更新日期:1995-05-08 00:00:00
abstract::Elongation factor EF-Tu from Escherichia coli was labelled with N-[14C]tosyl-L-phenylalanylchloromethane, digested with trypsin and the peptides obtained separated by HPLC. The only radioactive peak recovered corresponded to tryptic peptide containing residues 75-98. Sequencing of the peptide by automated Edman degrad...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)80795-3
更新日期:1982-12-27 00:00:00
abstract::The interaction of the adrenoleukodystrophy protein (ALDP), mutated in the peroxisomal disorder X-linked adrenoleukodystrophy, and the very long-chain acyl-CoA synthetase (VLACS), the enzyme whose function is missing in this disease, remains obscure. As a first step to studying this interaction in wild type versus ALD...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00255-5
更新日期:1998-03-27 00:00:00
abstract::Almost all eukaryotic mRNAs acquire a poly(A) tail at the 3'-end by a concerted RNA processing event: cleavage and polyadenylation. The canonical PAP, PAPα, was considered the only nuclear PAP involved in general polyadenylation of mRNAs. A phosphoinositide-modulated nuclear PAP, Star-PAP, was then reported to regulat...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.05.029
更新日期:2014-06-27 00:00:00
abstract::Read-through or mutations of a stop codon resulting in translation of the 3'-UTR produce potentially toxic C-terminally extended proteins. However, quality control mechanisms for such proteins are poorly understood in mammalian cells. Here, a comprehensive analysis of the 3'-UTRs of genes associated with hereditary di...
journal_title:FEBS letters
pub_type: 社论
doi:10.1002/1873-3468.13362
更新日期:2019-04-01 00:00:00
abstract::Mutations of the cysteines (positions 668 and 714) were generated in the IF2 C domain of Bacillus stearothermophilus translation initiation factor IF2. The corresponding proteins were characterized functionally and structurally. Most (yet not all) amino acid replacements at both positions resulted in severe reduction ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01280-6
更新日期:1999-10-15 00:00:00