cDNA cloning and mRNA distribution of a mouse very long-chain acyl-CoA synthetase.

abstract：:Phenylalanine-specific tRNA from yeast was hydrolysed with cobra venom ribonuclease in the double-stranded regions and the fragments isolated. The 'dissected' molecules with nicks in positions 28 and 41 were reconstructed from supplementary fragments and treated with T-4 RNA ligase. A phosphodiester bond between two f...

journal_title：FEBS letters

authors： Boutorin AS,Vassilenko SK,Baklanov MM,Nechaev YS

更新日期：1984-01-02 00:00:00

abstract：:Parts of the amino acid sequences of horse and dromedary pancreatic ribonuclease were reinvestigated. The sequence of residues 21-25 in horse ribonuclease is Ser-Asn-Pro-Thr-Tyr or Ser-Asn-Ser-Thr-Tyr. The asparagine in the latter sequence is glycosylated. Horse ribonuclease possesses four additional amino acid residu...

journal_title：FEBS letters

authors： Beintema JJ

更新日期：1985-06-03 00:00:00

abstract：:The small GTPase Rho1 plays an essential role in controlling the organization of the actin cytoskeleton and synthesis of the cell wall in the fission yeast Schizosaccharomyces pombe. Here we studied the role of Rho5 whose primary structure is very similar to that of Rho1. It was found that elevated expression of Rho5 ...

journal_title：FEBS letters

authors： Nakano K,Arai R,Mabuchi I

更新日期：2005-09-26 00:00:00

abstract：:Thrombin has been shown to inhibit skeletal muscle differentiation. However, the mechanisms by which thrombin represses myogenesis remain unknown. Since the thrombin receptor couples to G(i), G(q/11) and G(12), we examined which subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (Galpha(i), Galp...

journal_title：FEBS letters

authors： Nagao M,Kaziro Y,Itoh H

更新日期：2000-04-28 00:00:00

abstract：:Three novel neuropeptides, isolated from ganglia of the African giant snail, Achatina fulica, were named WWamide-1, -2 and -3. These substances were biologically active heptapeptide amides with a Trp residue at both the N- and C-termini. WWamide-1, which displayed an inhibitory activity on a central neuron of the snai...

journal_title：FEBS letters

authors： Minakata H,Ikeda T,Muneoka Y,Kobayashi M,Nomoto K

更新日期：1993-05-24 00:00:00

abstract：:Calcium release from internal stores of Entamoeba histolytica, a parasitic protozoan, was observed by measuring fluorescence of Fura-2. Emptying of inositol(1,4,5)trisphosphate (Ins(1,4,5)P3)-sensitive calcium pools in permeabilized E. histolytica did not significantly affect subsequent calcium release by inositol(1,3...

journal_title：FEBS letters

authors： Raha S,Giri B,Bhattacharyya B,Biswas BB

更新日期：1995-04-10 00:00:00

abstract：:A macromolecular complex containing survival of motor neurons (SMN), the spinal muscular atrophy protein, and Gemin2-7 interacts with Sm proteins and snRNAs to carry out the assembly of these components into spliceosomal small nuclear ribonucleoproteins (snRNPs). Here we report the characterization of unr-interacting ...

journal_title：FEBS letters

authors： Carissimi C,Baccon J,Straccia M,Chiarella P,Maiolica A,Sawyer A,Rappsilber J,Pellizzoni L

更新日期：2005-04-25 00:00:00

abstract：:We report a new member of the von Willebrand factor A-domain protein superfamily, WARP (for von Willebrand factor A-domain-related protein). The full-length mouse WARP cDNA is 2.3 kb in size and predicts a protein of 415 amino acids which contains a signal sequence, a VA-like domain, two fibronectin type III-like repe...

journal_title：FEBS letters

authors： Fitzgerald J,Tay Ting S,Bateman JF

更新日期：2002-04-24 00:00:00

abstract：:Hepatocyte tight junctional permeability has been shown to be regulated by hormones that exert their effects via phospholipase C activation. However, the precise transduction pathway involved in this effect is not known. The present study has employed the selective inhibitor of microsomal Ca2+ sequestration, 2,5-di(te...

journal_title：FEBS letters

authors： Llopis J,Kass GE,Duddy SK,Farell GC,Gahm A,Orrenius S

更新日期：1991-03-11 00:00:00

abstract：:Keratins form an intracellular keratin filament network in keratinocytes. Point mutations in the epidermal keratins could lead to the disruption of keratin filament formation, developing skin diseases such as epidermolytic hereditary palmoplantar keratoderma (EHPPK). We found a G to A transition in keratin 9 (K9) cDNA...

journal_title：FEBS letters

authors： Kobayashi S,Tanaka T,Matsuyoshi N,Imamura S

更新日期：1996-05-20 00:00:00

abstract：:The aim of this study was to investigate the distribution of a calcium-inhibitable adenylyl cyclase type VI (type VI AC) in the central nervous system using an antiserum directed against the N-terminus of type VI AC. Our results indicate that type VI AC immunoreactivity is generally expressed in many brain regions wit...

journal_title：FEBS letters

authors： Liu FC,Wu GC,Hsieh ST,Lai HL,Wang HF,Wang TW,Chern Y

更新日期：1998-09-25 00:00:00

abstract：:HMr alpha 2CPI was found to be an inhibitor of human liver cathepsin H by the measurement of the dissociation constant (Ki), the association rate constant (k1) and the dissociation rate constant (k-1) between the enzyme and the inhibitor. These data suggest that this protein-proteinase inhibitor can play a physiologic...

journal_title：FEBS letters

authors： Pagano M,Engler R

更新日期：1984-01-23 00:00:00

abstract：:Lipid transfer proteins (LTPs) and elicitins are both able to load and transfer lipidic molecules and share some structural and functional properties. While elicitins are known as elicitors of plant defence mechanisms, the biological function of LTP is still an enigma. We show that a wheat LTP1 binds with high affinit...

journal_title：FEBS letters

authors： Buhot N,Douliez JP,Jacquemard A,Marion D,Tran V,Maume BF,Milat ML,Ponchet M,Mikès V,Kader JC,Blein JP

更新日期：2001-11-30 00:00:00

abstract：:WT1 encodes a zinc finger protein with a key role in urogenital development that is inactivated in a subset of Wilms' tumors. This tumor suppressor gene product contains an amino-terminal dimerization domain required for trans-inhibition of wild-type WT1 activity by mutants defective for DNA binding. In the course of ...

journal_title：FEBS letters

authors： Bruening W,Moffett P,Chia S,Heinrich G,Pelletier J

更新日期：1996-09-09 00:00:00

abstract：:Annexin V is an alpha-helical protein which shows anticoagulatory and antiinflammatory activity. It is supposed to be involved in membrane fusion and exocytosis. In this study acid-induced equilibrium unfolding of the human annexin V is investigated by fluorescence and circular dichroism spectroscopy. The spectroscopi...

journal_title：FEBS letters

authors： Beermann ofm cap BB,Hinz HJ,Hofmann A,Huber R

更新日期：1998-02-20 00:00:00

abstract：:Disulfide bond formation is a critical step in the folding of many secretory proteins. In bacteria, disulfide bonds are introduced by the periplasmic dithiol oxidase DsbA, which transfers its catalytic disulfide bond to folding polypeptides. Reduced DsbA is reoxidized by ubiquinone Q8, catalyzed by inner membrane quin...

journal_title：FEBS letters

authors： Malojcić G,Owen RL,Grimshaw JP,Glockshuber R

更新日期：2008-10-15 00:00:00

abstract：:The tomato saponin alpha-tomatine has been proposed to kill sensitive cells by binding to cell membranes followed by leakage of cell components. However, details of the modes of action of the compound on fungal cells are poorly understood. In the present study, mechanisms involved in alpha-tomatine-induced cell death ...

journal_title：FEBS letters

authors： Ito S,Ihara T,Tamura H,Tanaka S,Ikeda T,Kajihara H,Dissanayake C,Abdel-Motaal FF,El-Sayed MA

更新日期：2007-07-10 00:00:00

abstract：:Mice deficient in granulocyte-macrophage colony stimulating factor (GM-CSF) develop pulmonary alveolar proteinosis (PAP). We found that bronchoalveolar lavage fluid (BALF) from 11 patients with idiopathic pulmonary alveolar proteinosis (IPAP) suppressed the growth of peripheral blood monocytes and TF-1 cells, a cell l...

journal_title：FEBS letters

authors： Tanaka N,Watanabe J,Kitamura T,Yamada Y,Kanegasaki S,Nakata K

更新日期：1999-01-15 00:00:00

abstract：:Many receptors coupled to inhibitory Go/Gi-type G proteins often also produce stimulatory signals like Ca2+ mobilisation. When expressed in CHO cells the alpha2-adrenoceptor subtypes alpha2A, alpha2B and alpkha2C mobilised Ca2+. These responses were strongly reduced by the P2Y-purinoceptor antagonist suramin. A large ...

journal_title：FEBS letters

authors： Akerman KE,Näsman J,Lund PE,Shariatmadari R,Kukkonen JP

更新日期：1998-07-03 00:00:00

abstract：:Clusters of codons pairing to low-abundance tRNAs synchronize the translation with co-translational folding of single domains in multidomain proteins. Although proven with some examples, the impact of the ribosomal speed on the folding and solubility on a global, cell-wide level remains elusive. Here we show that upre...

journal_title：FEBS letters

authors： Fedyunin I,Lehnhardt L,Böhmer N,Kaufmann P,Zhang G,Ignatova Z

更新日期：2012-09-21 00:00:00

abstract：:The effect of oxLDL on CD36 expression has been assessed in preadipocytes induced to differentiate. Novel evidence is provided that oxLDL induce a peroxisome proliferator-activated receptor gamma-independent CD36 overexpression, by up-regulating nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2). The nuclear t...

journal_title：FEBS letters

authors： D'Archivio M,Scazzocchio B,Filesi C,Varì R,Maggiorella MT,Sernicola L,Santangelo C,Giovannini C,Masella R

更新日期：2008-06-25 00:00:00

abstract：:It was of interest to examine whether yeast Cu-thionein could be used to transfer the thiolate bound copper directly into the copper binding site of 'blue' apoproteins which contain free thiol groups. In particular apo-stellacyanin was used in the present study and it was found to be able to accept Cu(I) from yeast Cu...

journal_title：FEBS letters

authors： Hartmann HJ,Morpurgo L,Desideri A,Rotilio G,Weser U

更新日期：1983-02-07 00:00:00

abstract：:Mammalian color vision is mediated by light-sensitive pigments in retinal cone cells. Biochemical studies on native mammalian cone visual pigments are seriously hampered by their low levels and instability. We describe a novel approach for their functional expression, employing the baculovirus system in combination wi...

journal_title：FEBS letters

authors： Vissers PM,DeGrip WJ

更新日期：1996-10-28 00:00:00

abstract：:Sterically hindered cyclic amines react with hydroxyl radicals in the presence of oxygen to yield stable nitroxide radicals which can be detected by EPR. This reaction provides a nonconventional spin-trapping tool for detection of hydroxyl radicals. ...

journal_title：FEBS letters

authors： Rosenthal I,Krishna CM,Yang GC,Kondo T,Riesz P

更新日期：1987-09-28 00:00:00

abstract：:Protease involvement has been implicated in the signalling process of activation-induced apoptosis. Here we report the isolation of a protease from Jurkat T cells undergoing Fas-induced apoptosis. Although the protease probably is a serine protease, it seems to be distantly related to members of the ICE/ced-3/Ich-1(ne...

journal_title：FEBS letters

authors： Schlegel J,Peters I,Orrenius S

更新日期：1995-05-08 00:00:00

abstract：:Three proteins of the mitochondrial inner membrane are known that are essential for the viability of yeast and seem to be involved in import of preproteins; the integral membrane proteins MIM17 and MIM23 and the peripheral membrane protein MIM44, MIM17 and MIM23 are homologous to each other in their hydrophobic domain...

journal_title：FEBS letters

authors： Kübrich M,Keil P,Rassow J,Dekker PJ,Blom J,Meijer M,Pfanner N

更新日期：1994-08-01 00:00:00

abstract：:The ubiquitous transcription factor Sp1 has been implicated in the mechanism which maintains CpG islands methylation-free. Plasmids containing GC boxes (Sp1 sites) were in vitro methylated at every CpG dinucleotide. After stable introduction into F9 embryonal carcinoma cells, we analysed the methylation of the sequenc...

journal_title：FEBS letters

authors： Silke J,Rother KI,Georgiev O,Schaffner W,Matsuo K

更新日期：1995-08-21 00:00:00

abstract：:The presence of protein kinase C (PKC), a key enzyme in signal transduction, has not been investigated in fungal cells. The phorbol ester TPA, an activator of PKC, may be used as an indicator of the presence and role of PKC in Phycomyces blakesleeanus spores. Activation of spore germination by acetate was prevented by...

journal_title：FEBS letters

authors： Carrillo-Rayas T,Garcia-Soto J,Martinez-Cadena G

更新日期：1988-10-10 00:00:00

abstract：:The effective synthesis of the envelope antigen F1 of Y. pestis in E. coli HB101 is mediated by the expression of the caf1M gene. This gene was sequenced, and the protein encoded was found to have a significant homology with the chaperone protein PapD of uropathogenic E. coli. The data presented allow one to suppose C...

journal_title：FEBS letters

authors： Galyov EE,Karlishev AV,Chernovskaya TV,Dolgikh DA,Smirnov OYu,Volkovoy KI,Abramov VM,Zav'yalov VP

更新日期：1991-07-29 00:00:00

abstract：:We have examined the ability of inositol 1,4,5-trisphosphate (InsP3) to cause contractions of mechanically skinned muscle fibres of frog and barnacle. InsP3 (10-500 microM) did not cause any tension development in 25 frog skinned fibres and 26 barnacle myofibrillar bundles, although contractions could be readily evoke...

journal_title：FEBS letters

authors： Lea TJ,Griffiths PJ,Tregear RT,Ashley CC

更新日期：1986-10-20 00:00:00