The light-harvesting core-complex and the B820-subunit from Rhodopseudomonas marina. Part I. Purification and characterisation.

abstract：:The atomic structures of K+ channels have added a new dimension to our understanding of K+ channel function. I will briefly review how structures have influenced our views on ion conduction, gating of the pore, and voltage sensing. ...

journal_title：FEBS letters

authors： MacKinnon R

更新日期：2003-11-27 00:00:00

abstract：:Oligopeptidase B (OpdB) from Trypanosoma brucei is a candidate therapeutic target in African trypanosomiasis. OpdB is an atypical serine peptidase, since activity is inhibited by thiol-blocking reagents and enhanced by reducing agents. We have identified C256 as the reactive cysteine residue that mediates OpdB inhibit...

journal_title：FEBS letters

authors： Morty RE,Shih AY,Fülöp V,Andrews NW

更新日期：2005-04-11 00:00:00

abstract：:Antigen-induced B cell activation requires mobilization of the Ca(2+) second messenger. This process is associated with the subcellular relocalization of signal effector proteins of the B cell antigen receptor such as the adaptor protein SLP65. Here we describe a broadly applicable live cell imaging method to simultan...

journal_title：FEBS letters

authors： Junek S,Engelke M,Schild D,Wienands J

更新日期：2012-05-21 00:00:00

abstract：:The aim of the present study is to determine the role of intracellular Ca(2+) in VEGF signaling. We demonstrate that reduction in Ca(2+) by chelating compound BAPTA-AM or by IP(3)-endoplasmic reticulum blocker 2-APB selectively inhibited VEGF-induced activation of c-Src-PI3K-Akt but not ERK1/2 in human coronary artery...

journal_title：FEBS letters

authors： Lee M,Spokes KC,Aird WC,Abid MR

更新日期：2010-07-16 00:00:00

abstract：:The activity of S-adenosylmethionine decarboxylase (AdoMetDC) in Crithidia fasciculata was shown to be correlated to the growth of the parasite. An increase in activity was observed during exponential growth. Inhibition of protein synthesis induced an extremely rapid decay of AdoMetDC activity. The half-life of the en...

journal_title：FEBS letters

authors： Nasizadeh S,Persson L

更新日期：2003-10-09 00:00:00

abstract：:Glycosylphosphatidylinositol (GPI) is synthesized in the endoplasmic reticulum (ER) and added onto proteins to form GPI-anchored proteins. Among the many proteins involved in this process, ACAT-related enzyme-2 required for viability 1 (Arv1) is a candidate, functioning as a flippase that translocates GPI intermediate...

journal_title：FEBS letters

authors： Okai H,Ikema R,Nakamura H,Kato M,Araki M,Mizuno A,Ikeda A,Renbaum P,Segel R,Funato K

更新日期：2020-08-01 00:00:00

abstract：:The mitochondrial permeability transition pore, a cyclosporin A-sensitive channel, is controlled by the transmembrane electric potential difference across the inner membrane. Here, we show that treatment of rat liver mitochondria with the arginine reagent phenylglyoxal inhibits the permeability transition pore trigger...

journal_title：FEBS letters

authors： Eriksson O,Fontaine E,Petronilli V,Bernardi P

更新日期：1997-06-16 00:00:00

abstract：:alphaArg-376, betaLys-155, and betaArg-182 are catalytically important ATP synthase residues that were proposed to be involved in substrate Pi binding and subsequent steps of ATP synthesis [Senior, A.E., Nadanaciva, S. and Weber, J. (2002) Biochim. Biophys. Acta 1553, 188-211]. Here, it was shown using purified Escher...

journal_title：FEBS letters

authors： Ahmad Z,Senior AE

更新日期：2005-01-17 00:00:00

abstract：:To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. 'AZT-resistant' mutations in RT were shown to be associated wi...

journal_title：FEBS letters

authors： Pokholok DK,Gudima SO,Yesipov DS,Dobrynin VN,Rechinsky VO,Kochetkov SN

更新日期：1993-07-05 00:00:00

abstract：:Both soluble and microsomal fractions of Fusarium oxysporum contain cytochrome P-450(P-450). We report here that the P-450 in the soluble fraction was induced only when nitrate or nitrite was added to the growth medium, whereas the microsomal P-450 was synthesized regardless of the medium compositions. The reduced-CO ...

journal_title：FEBS letters

authors： Shoun H,Suyama W,Yasui T

更新日期：1989-02-13 00:00:00

abstract：:The sequence of seminalplasmin, a basic antimicrobial and transcription-inhibitory protein from bovine seminal plasma, has been determined using an automated sequenator. This sequence is slightly different from that reported earlier by Theil and Scheit [(1983) EMBO J. 2, 1159-1163] and identical with that of caltrin, ...

journal_title：FEBS letters

authors： Sitaram N,Kumari VK,Bhargava PM

更新日期：1986-06-09 00:00:00

abstract：:The hexose transporter family of Saccharomyces cerevisiae comprises 18 proteins (Hxt1-17, Gal2). Here, we demonstrate that all these proteins, except Hxt12, and additionally three members of the maltose transporter family (Agt1, Ydl247, Yjr160) are able to transport hexoses. In a yeast strain deleted for HXT1-17, GAL2...

journal_title：FEBS letters

authors： Wieczorke R,Krampe S,Weierstall T,Freidel K,Hollenberg CP,Boles E

更新日期：1999-12-31 00:00:00

abstract：:The mechanism of stimulation of hepatic respiration by fatty acids was studied in isolated rat hepatocytes. Stimulation of respiration by fatty acids varied from about 35% to about 105% depending on chain length. The stimulatory effect of octanoate (1 mM) or oleate (0.5 mM) was prevented by oligomycin (2 micrograms/ml...

journal_title：FEBS letters

authors： Plomp PJ,van Roermund CW,Groen AK,Meijer AJ,Tager JM

更新日期：1985-12-02 00:00:00

abstract：:We present evidence that the potent chloride channel blocker NPPB has protonophoric activity in the mitochondria and across the plasma membrane of phagocytic cells. The resting O2 consumption of murine peritoneal macrophages was stimulated up to 2.5-fold in the presence of NPPB, with a K0.5 of 15 microM. The stimulato...

journal_title：FEBS letters

authors： Lukacs GL,Nanda A,Rotstein OD,Grinstein S

更新日期：1991-08-19 00:00:00

abstract：:Reconstitution of the 16 kDa N-terminal domain of the peridinin-chlorophyll-protein, N-PCP, with mixtures of chlorophyll a (Chl a) and Chl b, resulted in 32 kDa complexes containing two pigment clusters, each bound to one N-PCP. Besides homo-chlorophyllous complexes, hetero-chlorophyllous ones were obtained that conta...

journal_title：FEBS letters

authors： Brotosudarmo TH,Hofmann E,Hiller RG,Wörmke S,Mackowski S,Zumbusch A,Bräuchle C,Scheer H

更新日期：2006-10-02 00:00:00

abstract：:Dark-grown angiosperm seedlings lack chlorophylls, but accumulate protochlorophyllide a complexed with the light-dependent enzyme NADPH:protochlorophyllide oxidoreductase. Previous investigators correlated spectral heterogeneity of in vivo protochlorophyllide forms and a shift of chlorophyllide forms from 680 to 672 n...

journal_title：FEBS letters

authors： Klement H,Oster U,Rüdiger W

更新日期：2000-09-01 00:00:00

abstract：:Accumulation of hyperphosphorylated Tau is associated with a number of neurodegenerative diseases collectively known as tauopathies. Differences in clinical and cognitive profiles among them suggest differential sensitivity of neuronal populations to Tau levels, phosphorylation and mutations. We used tissue specific e...

journal_title：FEBS letters

authors： Grammenoudi S,Kosmidis S,Skoulakis EM

更新日期：2006-08-21 00:00:00

abstract：:We report that sodium butyrate, a natural product of fiber degradation by colonic bacteria, markedly suppresses c-Myc-mediated apoptosis induced in murine plasmacytomas and human Burkitt lymphomas by growth factor deprivation, but not in cell lines devoid of c-myc translocations. Attenuation of cell death is associate...

journal_title：FEBS letters

authors： Alexandrov I,Romanova L,Mushinski F,Nordan R

更新日期：1998-08-28 00:00:00

abstract：:Friedreich's ataxia is caused by a deficit in the mitochondrial protein frataxin. The present work demonstrates that in vivo yeast frataxin Yfh1p and Isu1p, the mitochondrial scaffold protein for the Fe-S cluster assembly, have tightly linked biological functions, acting in concert to promote the Fe-S cluster assembly...

journal_title：FEBS letters

authors： Ramazzotti A,Vanmansart V,Foury F

更新日期：2004-01-16 00:00:00

abstract：:The dynamics of changes in membrane potential, cytosolic free Ca2+, [Ca2+]i and immunoreactive insulin release were assessed in RINm5F cells. Membrane depolarization and a rise in [Ca2+]i preceded the stimulation of insulin release by D-glyceraldehyde. Forskolin, which raised the cellular cyclic AMP levels, stimulated...

journal_title：FEBS letters

authors： Wollheim CB,Ullrich S,Pozzan T

更新日期：1984-11-05 00:00:00

abstract：:As the initiator of DNA double-strand breaks during meiosis in Saccharomyces cerevisiae, the SPO11 protein is essential for recombination. Similarity between SPO11 and archaebacterial TOP6A proteins points to evolutionary specialization of a DNA cleavage function for meiotic recombination. To determine whether this ex...

journal_title：FEBS letters

authors： Shannon M,Richardson L,Christian A,Handel MA,Thelen MP

更新日期：1999-12-03 00:00:00

abstract：:We used Xenopus oocytes co-expressing thyrotropin-releasing hormone (TRH) receptors and human ether-a-go-go-related gene (HERG) K+ channel variants carrying different amino-terminal modifications to check the relevance of the proximal domain for hormonal regulation of the channel. Deletion of the whole proximal domain...

journal_title：FEBS letters

authors： Gómez-Varela D,Barros F,Viloria CG,Giráldez T,Manso DG,Dupuy SG,Miranda P,de la Peña P

更新日期：2003-01-30 00:00:00

abstract：:Post-transcriptional regulation of gene expression by RNA-binding proteins and by small non-coding RNAs plays an important role in cell biology. Our previous results show that in murine skeletal myoblasts, the expression of Pinch-2, a focal adhesion remodeling factor that regulates cell motility, is repressed by an RN...

journal_title：FEBS letters

authors： Boudoukha S,Rivera Vargas T,Dang I,Kropp J,Cuvellier S,Gautreau A,Polesskaya A

更新日期：2014-05-02 00:00:00

abstract：:cDNAs encoding rat adipose tissue hormone-sensitive lipase were expressed in COS cells, under the control of the SV40 promoter to half the level in rat adipocytes, the richest native source of the enzyme. A cDNA lacking most of the long 5'-untranslated region of the full-length rat hormone-sensitive lipase cDNA was, w...

journal_title：FEBS letters

authors： Holm C,Davis RC,Fredrikson G,Belfrage P,Schotz MC

更新日期：1991-07-08 00:00:00

abstract：:In hydra the neuropeptide head activator (HA) is responsible for head-specific growth and differentiation processes. The effects of HA are mediated by high and low affinity receptors on hydra cells. In the current study HA receptors from a multi-headed mutant of Chlorohydra viridissima were solubilized from the membra...

journal_title：FEBS letters

authors： Christians S,Neubauer KH,Ulrich H

更新日期：1993-01-25 00:00:00

abstract：:The muscular content of the mRNAs encoding the five subunits of the nicotonic acetylcholine receptor was measured during postnatal development in the rat. Subunit specific mRNAs show differential regulation. The levels of the alpha-, gamma- and delta-subunit specific mRNAs decrease steadily after birth, while the beta...

journal_title：FEBS letters

authors： Witzemann V,Barg B,Criado M,Stein E,Sakmann B

更新日期：1989-01-02 00:00:00

abstract：:G-quadruplex structures in the 5' UTR of mRNAs have been shown to suppress translation. However, all experiments published so far made use of artificial reporter systems. Here, we investigate for the first time the biological effects of the genomic disruption of a G-quadruplex by CRISPR/Cas9. In melanoma cells lacking...

journal_title：FEBS letters

authors： Serikawa T,Eberle J,Kurreck J

更新日期：2017-11-01 00:00:00

abstract：:Tripeptidyl peptidase I (TPP-I) is a lysosomal enzyme that cleaves tripeptides from the N-terminus of polypeptides. A comparison of TPP-I amino acid sequences with sequences derived from an EST database suggested that TPP-I is identical to a pepstatin-insensitive carboxyl proteinase of unknown specificity which is mut...

journal_title：FEBS letters

authors： Vines DJ,Warburton MJ

更新日期：1999-01-25 00:00:00

abstract：:Contribution of phosphatidic acid (PA) generated by activated phospholipase (PL) D to PLA2 activation was studied in rat peritoneal mast cells. Exogenous didecanoyl PA induced arachidonate liberation in the permeabilized cells which was inhibited by p-bromophenacyl bromide. Upon exposure of the cells to ethanol in a h...

journal_title：FEBS letters

authors： Sato T,Ishimoto T,Akiba S,Fujii T

更新日期：1993-05-24 00:00:00

abstract：:Recombinant cell lines expressing D2 and D3 dopamine receptor isoforms have been used to study coupling of these receptors to G-proteins from the effects of GTP to reduce agonist binding affinities in agonist/[3H]spiperone competition experiments. D2(short) expressed in Ltk- cells, D2(long) expressed in Ltk- or CHO ce...

journal_title：FEBS letters

authors： Castro SW,Strange PG

更新日期：1993-01-11 00:00:00