Spatiotemporal resolution of Ca2+ signaling events by real time imaging of single B cells.

abstract：:The mammalian gonadotropin-releasing hormone (GnRH-I), which regulates reproduction, was the first isoform of GnRH that was identified in mammals. Recently, we and others have demonstrated the existence of a second isoform of GnRH in the brain of mammals. The presence of a third isoform of GnRH, GnRH-III, in the brain...

journal_title：FEBS letters

authors： Yahalom D,Chen A,Ben-Aroya N,Rahimipour S,Kaganovsky E,Okon E,Fridkin M,Koch Y

更新日期：1999-12-17 00:00:00

abstract：:This study was undertaken to demonstrate the unique specificity of the chemokine receptor CXCR4 antagonist AMD3100. Calcium flux assays with selected chemokine/cell combinations, affording distinct chemokine receptor specificities, revealed no interaction of AMD3100 with any of the chemokine receptors CXCR1 through CX...

journal_title：FEBS letters

authors： Hatse S,Princen K,Bridger G,De Clercq E,Schols D

更新日期：2002-09-11 00:00:00

abstract：:A method was established for measuring molecule numbers of three different myosin heavy chain (MHC) mRNA isoforms in total RNA preparations. The quantification was based on a combination of primer-directed reverse transcriptase and polymerase chain reactions with 5'-digoxigenin-labeled oligonucleotides, using external...

journal_title：FEBS letters

authors： Peuker H,Pette D

更新日期：1993-03-08 00:00:00

abstract：:The trans----cis isomerization of Pro 93 was measured during refolding of bovine ribonuclease A. This isomerization is slow (tau = 500 s) under marginally stable folding conditions of 2.0 M GdmCl, pH 6, at 10 degrees C. However, it is strongly accelerated (tau = 100 s) in samples which, prior to isomerization, had bee...

journal_title：FEBS letters

authors： Schmid FX

更新日期：1986-03-31 00:00:00

abstract：:In the new view, hydrophobic free energy is measured by the work of solute transfer of hydrocarbon gases from vapor to aqueous solution. Reasons are given for believing that older values, measured by solute transfer from a reference solvent to water, are not quantitatively correct. The hydrophobic free energy from gas...

journal_title：FEBS letters

authors： Baldwin RL

更新日期：2013-04-17 00:00:00

abstract：:Chronic indirect stimulation of fast twitch rabbit muscle induced a rapid reduction in parvalbumin concentration. When compared to the unstimulated contralateral muscle, parvalbumins were reduced to 55% following 6 days of stimulation. Prolonged stimulation further reduced parvalbumins so that they were undetectable a...

journal_title：FEBS letters

authors： Klug G,Reichmann H,Pette D

更新日期：1983-02-21 00:00:00

abstract：:Alterations in both calcitonin (CT) secretion and plasma calcium were recently described in adult obese Zucker rats. We have investigated the CT biosynthetic activity of thyroid glands in 30-day-old obese Zucker rats (fa/fa), and their controls (Lean). Plasma calcium level was significantly increased (+0.6 mg/dl) in o...

journal_title：FEBS letters

authors： Segond N,Jullienne A,Tahri EH,Garel JM

更新日期：1984-08-20 00:00:00

abstract：:Apolipoprotein E (APOE) genotype determines Alzheimer's disease (AD) susceptibility, with the APOE ε4 allele being an established risk factor for late-onset AD. The ApoE lipidation status has been reported to impact amyloid-beta (Aβ) peptide metabolism. The details of how lipidation affects ApoE behavior remain to be ...

journal_title：FEBS letters

authors： Hubin E,Verghese PB,van Nuland N,Broersen K

更新日期：2019-06-01 00:00:00

abstract：:The type 1 ribosome-inactivating protein (RIP) saporin 5 isolated from seeds of Saponaria officinalis L. strongly inhibited translation carried out by Vicia sativa L. purified ribosomes. The toxin multidepurinated V. sativa rRNA, which upon treatment with acid aniline releases several RNA fragments including an RNA fr...

journal_title：FEBS letters

authors： Iglesias R,Arias FJ,Rojo MA,Escarmis C,Ferreras JM,Girbés T

更新日期：1993-07-05 00:00:00

abstract：:HERC proteins are characterized by having one or more RCC1-like domains as well as a C-terminal HECT domain in their amino acid sequences. This has led researchers to suggest that they may act as both guanine nucleotide exchange factors and E3 ubiquitin ligases. Here we describe a physical interaction between the HECT...

journal_title：FEBS letters

authors： Garcia-Gonzalo FR,Cruz C,Muñoz P,Mazurek S,Eigenbrodt E,Ventura F,Bartrons R,Rosa JL

更新日期：2003-03-27 00:00:00

abstract：:Two new three-disulfide intermediates have been found to be populated in the oxidative folding pathway of bovine pancreatic ribonuclease A at a low temperature (15 degrees C). These intermediates, des-[26-84] and des-[58-110], possess all but one of the four native disulfide bonds and have a stable tertiary structure,...

journal_title：FEBS letters

authors： Welker E,Narayan M,Volles MJ,Scheraga HA

更新日期：1999-11-05 00:00:00

abstract：:Accumulating evidence has indicated that microRNAs (miRNAs) act as critical epigenetic regulators in tumor carcinogenesis. Here, we report that miR-199a-3p was significantly upregulated in gastric cancer (GC) cell lines and tissues. Functional studies demonstrated that miR-199a-3p dramatically increased cell prolifera...

journal_title：FEBS letters

authors： Wang Z,Ma X,Cai Q,Wang X,Yu B,Cai Q,liu B,Zhu Z,Li C

更新日期：2014-11-28 00:00:00

abstract：:Tissue plasminogen activator was purified in high yield from pig heart by immunoaffinity chromatography and characterized by analysis of the glycosylation pattern and the N-terminal amino acid sequence. Comparisons with the human enzyme reveals residue exchanges in the A-chain at positions 3 (porcine Arg/human Gln) an...

journal_title：FEBS letters

authors： Pohl G,Jörnvall H,Kok P,Wallén P

更新日期：1986-09-01 00:00:00

abstract：:We have found that N-acetylneuraminic acid (NANA) consumes toxic hydrogen peroxide (H(2)O(2)) under physiological conditions. Close investigation of this finding revealed that NANA was oxidized by an equimolar amount of H(2)O(2) to provide its decarboxylated product, 4-(acetylamino)-2,4-dideoxy-D-glycero-D-galacto-oct...

journal_title：FEBS letters

authors： Iijima R,Takahashi H,Namme R,Ikegami S,Yamazaki M

更新日期：2004-03-12 00:00:00

abstract：:Stimulated production of reactive oxygen species (ROS) by plasma membrane-associated nicotinamide adenine dinucleotide phosphate oxidases (Nox) in non-phagocytic cells regulates a number of biological processes including growth, vessel tone, and oxygen sensing. The purpose of this study was to investigate H(2)O(2)-sti...

journal_title：FEBS letters

authors： Colston JT,de la Rosa SD,Strader JR,Anderson MA,Freeman GL

更新日期：2005-04-25 00:00:00

abstract：:Glycine amide (GlyAd), a typically amidated amino acid, is a versatile additive that suppresses protein aggregation during refolding, heat treatment, and crystallization. In spite of its effectiveness, the exact mechanism by which GlyAd suppresses protein aggregation remains to be elucidated. Here, we show the crystal...

journal_title：FEBS letters

authors： Ito L,Shiraki K,Makino M,Hasegawa K,Kumasaka T

更新日期：2011-02-04 00:00:00

abstract：:Lymphotoxin-beta receptor (LTbetaR) is a member of the tumor necrosis factor receptor (TNFR) superfamily that activates nuclear factor-kappaB (NF-kappaB) through the IkappaB kinase (IKK) complex, the core of which is comprised of IKK1, IKK2 and NF-kappaB essential modulator (NEMO). We demonstrate here that the LTbetaR...

journal_title：FEBS letters

authors： Saitoh T,Nakano H,Yamamoto N,Yamaoka S

更新日期：2002-12-04 00:00:00

abstract：:We have isolated cDNA molecules encoding a protein with the characteristic sequence elements that are conserved between the catalytic domains of protein kinases. This protein is apparently a serine/threonine kinase and is most closely related to the amino-terminal half of the ribosomal protein S6 kinase II first chara...

journal_title：FEBS letters

authors： Harmann B,Kilimann MW

更新日期：1990-10-29 00:00:00

abstract：:The role of protein kinase C (PKC) and p42(MAP kinase) signaling in the regulation of proliferation and apoptosis was investigated in freshly isolated and primary cultured rat hepatocytes. Acute treatment of freshly isolated hepatocytes with phenylephrine and EGF caused rapid phasic activations of p42(MAP kinase) and ...

journal_title：FEBS letters

authors： Jarvis WD,Auer KL,Spector M,Kunos G,Grant S,Hylemon P,Mikkelsen R,Dent P

更新日期：1997-07-21 00:00:00

abstract：:TLR3 and TLR4 utilize adaptor TRIF to activate interferon regulatory factor 3 (IRF3), resulting in interferon β (IFN-β) production to mediate anti-viral infection. In this report, we analyzed the effect of two known phosphatidylinositol 3-kinase (PI3K) inhibitors LY294002 and wortmannin on LPS- and poly(I:C)-induced I...

journal_title：FEBS letters

authors： Zhao W,Qi J,Wang L,Zhang M,Wang P,Gao C

更新日期：2012-03-23 00:00:00

abstract：:pMNEI, a single chain sweet protein related to monellin, has been studied by means of 1H NMR at 500 MHz. A partial sequential assignment performed by means of the MCD method allowed the determination of the secondary structure of a large portion of the beta-sheet of pMNEI that contains a likely 'sweet finger': the loo...

journal_title：FEBS letters

authors： Tancredi T,Iijima H,Saviano G,Amodeo P,Temussi PA

更新日期：1992-09-21 00:00:00

abstract：:A conformational transition between E2 and E1 forms of Na, K-ATPase induced by different nucleotides has been studied under steady state conditions using the enzyme labelled with 5-iodoacetamidofluorescein. In the presence of K+ the plot of fluorescence as a function of [ATP], [ADP] or [CTP] (in a range of 5 microM-12...

journal_title：FEBS letters

authors： Lopina OD,Pindel EV,Boldyrev AA

更新日期：1990-06-18 00:00:00

abstract：:Zymosan, which is composed primarily of alpha-mannan and beta-glucan polymers, is a well recognized activator of macrophages. The type receptor by which unopsonized zymosan induces arachidonic acid release was investigated. It was found that particulate beta-glucan and zymosan stimulated an identical dose-dependent re...

journal_title：FEBS letters

authors： Daum T,Rohrbach MS

更新日期：1992-09-07 00:00:00

abstract：:The structure of the D158E mutant of caricain (previously known as papaya protease omega) in complex with E-64 has been determined at 2.0 A resolution (overall R factor 19.3%). The structure reveals that the substituted glutamate makes the same pattern of hydrogen bonds as the aspartate in native caricain. This was no...

journal_title：FEBS letters

authors： Katerelos NA,Taylor MA,Scott M,Goodenough PW,Pickersgill RW

更新日期：1996-08-19 00:00:00

abstract：:This paper reviews our present knowledge of sphingomyelinases as enzymes, and as enzymes acting on a membrane constituent lipid, sphingomyelin. Six types of sphingomyelinases are considered, namely acidic, secretory, Mg(2+)-dependent neutral, Mg(2+)-independent neutral, alkaline, and bacterial enzymes with both phosph...

journal_title：FEBS letters

authors： Goñi FM,Alonso A

更新日期：2002-10-30 00:00:00

abstract：:The effect of Rhodococcus fascians, the causal agent of leafy gall disease, on the mitotic behavior of synchronized tobacco Bright Yellow-2 (BY-2) cells was investigated. Incubation of aphidicolin-synchronized BY-2 cells with R. fascians cells specifically resulted in a broader mitotic index peak, an effect that was l...

journal_title：FEBS letters

authors： Temmerman W,Ritsema T,Simón-Mateo C,Van Montagu M,Mironov V,Inzé D,Goethals K,Holsters M

更新日期：2001-03-09 00:00:00

abstract：:The molecular mass of the rabbit prolactin receptor (rbPRLR) deduced from cDNA cloning is 66 kDa. However, the molecular mass of the full-length receptor expressed in the insect Sf9 cells was found to be 94 kDa. In order to explain this discrepancy, we analyzed the possible post-translational modifications of the PRLR...

journal_title：FEBS letters

authors： Cahoreau C,Garnier L,Djiane J,Devauchelle G,Cerutti M

更新日期：1994-08-22 00:00:00

abstract：:Structural gene expression of human immunodeficiency virus type 1 (HIV-1) requires a viral regulatory protein, Rev transactivator. We investigated Rev-dependency of HIV-1 gene expression by various reporter systems. Expression of unspliced and single-spliced viral mRNAs was demonstrated to be differentially dependent ...

journal_title：FEBS letters

authors： Sakai H,Furuta RA,Tokunaga K,Kawamura M,Hatanaka M,Adachi A

更新日期：1995-05-29 00:00:00

abstract：:Analogues of S-prenylated cysteine like N-acetyl-S-trans,trans-farnesyl-L-cysteine (AFC) have previously been shown to inhibit the carboxyl methylation of proteins carrying a C-terminal S-prenylated cysteine residue and to block the endotoxin-activated serum-elicited chemotactic response of mouse macrophages. Here, we...

journal_title：FEBS letters

authors： Scheer A,Gierschik P

更新日期：1993-03-15 00:00:00

abstract：:Stimuli which enhance secretion from parathyroid cells such as low extracellular Ca2+ or Mg2+ are associated with a decrease in the cytosolic Ca2+ concentration as measured by quin2. Current evidence suggests that increased production of inositol 1,4,5-triphosphate (IP3) releases Ca2+ from cellular stores thus increas...

journal_title：FEBS letters

authors： Epstein PA,Prentki M,Attie MF

更新日期：1985-08-19 00:00:00