Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae.

abstract：:Antibodies were raised, in rabbits, against an arachidonate- and U46619-binding protein purified from calf platelets. Spectral measurements and immunodiffusion experiments were employed to follow conformational responses of the protein in relation to platelet activation. Upon treatment with the platelet agonists, arac...

journal_title：FEBS letters

authors： Krishnan LK,Jamaluddin MP

更新日期：1987-02-23 00:00:00

abstract：:Tea-catechin derivatives are shown to inhibit activities of caspases-3, 2 and 7 in vitro, and prevented experimental apoptosis at the cell and animal levels. Epigallo-catechin-gallate showed the strongest inhibition at 1 x 10(-7)M to these caspases, but cysteine cathepsins and caspase-8 were not inhibited. Caspase-3 i...

journal_title：FEBS letters

authors： Katunuma N,Ohashi A,Sano E,Ishimaru N,Hayashi Y,Murata E

更新日期：2006-02-06 00:00:00

abstract：:Perhydrohistrionicotoxin at micromolar concentrations blocked the nicotine-evoked transmitter release from perfused striatal (dopaminergic) and hippocampal (cholinergic) nerve terminals. Perhydrohistrionicotoxin failed to compete with [3H]nicotine for its high-affinity binding site in rat brain, suggesting that the ac...

journal_title：FEBS letters

authors： Rapier C,Wonnacott S,Lunt GG,Albuquerque EX

更新日期：1987-02-23 00:00:00

abstract：:We have characterized the cell surface of zebrafish stratified epithelium using a combined approach of light and atomic force microscopy under conditions which simulate wound healing. Microridges rise on average 100 nm above the surface of living epithelial cells, which correlate to bundles of cytochalasin B-insensiti...

journal_title：FEBS letters

authors： Sharma A,Anderson KI,Müller DJ

更新日期：2005-03-28 00:00:00

abstract：:WT1 was isolated as a tumor suppressor gene of Wilms tumor. However, high expression of WT1 correlates with poor prognosis in acute leukemia. In addition suppression of WT1 expression by WT1 anti-sense oligonucleotide inhibits proliferation of leukemia cells, suggesting that WT1 is important for their proliferation. T...

journal_title：FEBS letters

authors： Murata Y,Kudoh T,Sugiyama H,Toyoshima K,Akiyama T

更新日期：1997-06-02 00:00:00

abstract：:trans-3-Chloroacrylic acid dehalogenase (CaaD) catalyzes the hydrolytic dehalogenation of trans-3-haloacrylates to yield malonate semialdehyde by a mechanism utilizing βPro-1, αArg-8, αArg-11, and αGlu-52. These residues are implicated in a promiscuous hydratase activity where 2-oxo-3-pentynoate is processed to acetop...

journal_title：FEBS letters

authors： Poelarends GJ,Serrano H,Huddleston JP,Johnson WH Jr,Whitman CP

更新日期：2013-09-02 00:00:00

abstract：:Daunorubicin induces apoptosis in myeloid leukemia cells by activation of neutral sphingomyelinase and ceramide generation occurring 4-10 min after daunorubicin addition. We show here that daunorubicin is able to increase the phosphoinositide 3-kinase activity and enhance intracellular phosphoinositide 3-kinase lipid ...

journal_title：FEBS letters

authors： Plo I,Bettaïeb A,Payrastre B,Mansat-De Mas V,Bordier C,Rousse A,Kowalski-Chauvel A,Laurent G,Lautier D

更新日期：1999-06-11 00:00:00

abstract：:We constructed five mutated cDNAs encoding human interferon-gamma (IFN-gamma) derivatives lacking 19-23 COOH-terminal residues and expressed them in Escherichia coli. All the derivatives were purified to homogeneity. They showed substantially the same order of antiviral activity in vitro as the intact molecule, and be...

journal_title：FEBS letters

authors： Sakaguchi M,Honda S,Ozawa M,Nishimura O

更新日期：1988-03-28 00:00:00

abstract：:The known members of inhibitor of growth (ING) gene family are considered as candidate tumor suppressor genes. ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53, p300 (a major component of histone acetyl transferase complexes), and p65(RelA) subunit of NF-kappaB. In this st...

journal_title：FEBS letters

authors： Zhang X,Xu LS,Wang ZQ,Wang KS,Li N,Cheng ZH,Huang SZ,Wei DZ,Han ZG

更新日期：2004-07-16 00:00:00

abstract：:To identify the novel genes involved in lipid metabolism and lipid droplet formation that may play important roles in Hepatitis C virus (HCV) propagation, we have screened the small interfering RNA library using cell culture derived HCV (HCVcc)-infected cells. We selected and characterized the gene encoding farnesyl-d...

journal_title：FEBS letters

authors： Park EM,Nguyen LN,Lim YS,Hwang SB

更新日期：2014-05-02 00:00:00

abstract：:Polyinosinic-polycytidylic acid, a potent inducer of inducer of interferon (IFN) production and activator of some IFN-induced enzymes, inhibits [3H]uridine incorporation into the RNA of vesicular stomatitis virus even in the absence of IFN synthesis, transiently triggers the breakdown of inositol phospholipids and act...

journal_title：FEBS letters

authors： Premecz G,Markovits A,Bagi G,Farkas T,Földes I

更新日期：1987-12-21 00:00:00

abstract：:Antigen-induced B cell activation requires mobilization of the Ca(2+) second messenger. This process is associated with the subcellular relocalization of signal effector proteins of the B cell antigen receptor such as the adaptor protein SLP65. Here we describe a broadly applicable live cell imaging method to simultan...

journal_title：FEBS letters

authors： Junek S,Engelke M,Schild D,Wienands J

更新日期：2012-05-21 00:00:00

abstract：:Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the nar...

journal_title：FEBS letters

authors： Roux M,Champeil P

更新日期：1984-06-11 00:00:00

abstract：:Gene duplication provides genetic material required for functional diversification. An interesting example is the amyloid precursor protein (APP) protein family. The APP gene family has experienced both expansion and contraction during evolution. The three mammalian members have been studied quite extensively in combi...

journal_title：FEBS letters

authors： Shariati SA,De Strooper B

更新日期：2013-06-27 00:00:00

abstract：:A tetradecapeptide with a sequence identical to residues 26-39 of the cro protein from bacteriophage lambda has been synthesized. This peptide has no secondary structure in an aqueous buffer but adopts an alpha-helical conformation in the presence of 20% hexafluoroisopropanol. The fluorescence of the single tyrosyl re...

journal_title：FEBS letters

authors： Mayer R,Lancelot G,Hélène C

更新日期：1983-03-21 00:00:00

abstract：:Purified antibodies against guanylic acid and guanosine binding to RNA at guanosine residues were used to probe human lymphocyte preparations by indirect immunofluorescence. Neither antibody gave any banding pattern with metaphase chromosomes but both showed binding to specific sites in the interphase nuclei. Evidence...

journal_title：FEBS letters

authors： Iswari S,Krishnamurthy DS,Jacob TM

更新日期：1985-09-23 00:00:00

abstract：:The isocitrate lyase-encoding gene AgICL1 from the filamentous hemiascomycete Ashbya gossypii was isolated by heterologous complementation of a Saccharomyces cerevisiae icl1d mutant. The open reading frame of 1680 bp encoded a protein of 560 amino acids with a calculated molecular weight of 62584. Disruption of the Ag...

journal_title：FEBS letters

authors： Maeting I,Schmidt G,Sahm H,Revuelta JL,Stierhof YD,Stahmann KP

更新日期：1999-02-05 00:00:00

abstract：:Reconstitution of the 16 kDa N-terminal domain of the peridinin-chlorophyll-protein, N-PCP, with mixtures of chlorophyll a (Chl a) and Chl b, resulted in 32 kDa complexes containing two pigment clusters, each bound to one N-PCP. Besides homo-chlorophyllous complexes, hetero-chlorophyllous ones were obtained that conta...

journal_title：FEBS letters

authors： Brotosudarmo TH,Hofmann E,Hiller RG,Wörmke S,Mackowski S,Zumbusch A,Bräuchle C,Scheer H

更新日期：2006-10-02 00:00:00

abstract：:The yeast Saccharomyces cerevisiae contains two acetyl-CoA synthetase genes, ACS1 and ACS2. While ACS1 transcription is glucose repressible, ACS2 shows coregulation with structural genes of fatty acid biosynthesis. The ACS2 upstream region contains an ICRE (inositol/choline-responsive element) as an activating sequenc...

journal_title：FEBS letters

authors： Hiesinger M,Wagner C,Schüller HJ

更新日期：1997-09-22 00:00:00

abstract：:The transmission of receptor-generated cytoplasmic Ca2+ signals between communicating pancreatic acinar cells has been investigated by comparing patch-clamp recordings of Ca(2+)-dependent Cl- current in internally perfused single cells and small multi-cell clusters. Acetylcholine (50 nM) generates shortlasting repetit...

journal_title：FEBS letters

authors： Petersen CC,Petersen OH

更新日期：1991-06-17 00:00:00

abstract：:A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase gene family, designated GalNAc-T7, was cloned and expressed. GalNAc-T7 exhibited different properties compared to other characterized members of this gene family, in showing apparent exclusive specificity for partial...

journal_title：FEBS letters

authors： Bennett EP,Hassan H,Hollingsworth MA,Clausen H

更新日期：1999-10-29 00:00:00

abstract：:Understanding protein beta structures has been hindered by the challenge of designing small, well-folded β-sheet systems. A β-capping motif was previously designed to help solve this problem, but not without limitations, as the termini of this β-cap were not fully available for chain extension. Combining Coulombic sid...

journal_title：FEBS letters

authors： Anderson JM,Kier BL,Shcherbakov AA,Andersen NH

更新日期：2014-12-20 00:00:00

abstract：:Small interfering (si)RNAs isolated from Brassica juncea leaves infected by Turnip mosaic virus (TuMV) were characterized by cloning and sequencing. The TuMV siRNA population was dominated by 21 and 22-nt long species originated mainly from the same siRNA hotspots, indicating operational similarity between the plant D...

journal_title：FEBS letters

authors： Ho T,Wang H,Pallett D,Dalmay T

更新日期：2007-07-10 00:00:00

abstract：:A hybrid protein from Neisseria meningitidis, which contains both a peroxiredoxin and a glutaredoxin domain, has been isolated. The enzyme was active in the reduction of various peroxides and dehydroascorbate in the presence of reduced glutathione. These findings suggest that both the peroxiredoxin and glutaredoxin do...

journal_title：FEBS letters

authors： Rouhier N,Jacquot JP

更新日期：2003-11-06 00:00:00

abstract：:An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-beta-galactosidase, followed by treatment with N-a...

journal_title：FEBS letters

authors： Hokke CH,Kamerling JP,van Dedem GW,Vliegenthart JF

更新日期：1991-07-29 00:00:00

abstract：:Calf chymosin molecules exist in the two alternative structural forms: the first one has S1 and S3 binding pockets occluded by its own Tyr77 residue (the self-inhibited form); the second has these pockets free for a substrate binding (the active form). The preliminary incubation of the enzyme with a pentapeptide corre...

journal_title：FEBS letters

authors： Gustchina E,Rumsh L,Ginodman L,Majer P,Andreeva N

更新日期：1996-01-22 00:00:00

abstract：:Expression plasmids encoding native human preporenin and a mutant deleted in its entire prosegment were transfected into Chinese hamster ovary cells. The cells transfected with the expression plasmid of native preporenin secreted exclusively inactive prorenin, while the cells transfected with the mutant secreted the a...

journal_title：FEBS letters

authors： Nagahama M,Nakayama K,Hori H,Murakami K

更新日期：1989-12-18 00:00:00

abstract：:Nine of the cbi genes from the 17.5 kb cob operon of Salmonella typhimurium previously shown by genetic studies to be involved in the biosynthesis of cobinamide from precorrin-2, have been subcloned and expressed in Escherichia coli. Seven of the gene products were found in the soluble fraction of cell lysates and hav...

journal_title：FEBS letters

authors： Roessner CA,Warren MJ,Santander PJ,Atshaves BP,Ozaki S,Stolowich NJ,Iida K,Scott AI

更新日期：1992-04-13 00:00:00

abstract：:MeCP2 is a chromatin-associated protein that is mutated in Rett syndrome. Its methyl-CpG-binding domain interacts with DNA containing methylated cytosine, but other modes of recruitment to the genome have also been proposed. Here, we use in vitro and in vivo assays to investigate the DNA binding specificity of two AT-...

journal_title：FEBS letters

authors： Lyst MJ,Connelly J,Merusi C,Bird A

更新日期：2016-09-01 00:00:00

abstract：:Prominin-1 (CD133) is a cholesterol-interacting pentaspan membrane protein concentrated in plasma membrane protrusions. In epithelial cells, notably neuroepithelial stem cells, prominin-1 is found in microvilli, the primary cilium and the midbody. These three types of apical membrane protrusions are subject to remodel...

journal_title：FEBS letters

authors： Corbeil D,Marzesco AM,Wilsch-Bräuninger M,Huttner WB

更新日期：2010-05-03 00:00:00