Abstract:
:We constructed five mutated cDNAs encoding human interferon-gamma (IFN-gamma) derivatives lacking 19-23 COOH-terminal residues and expressed them in Escherichia coli. All the derivatives were purified to homogeneity. They showed substantially the same order of antiviral activity in vitro as the intact molecule, and behaved as a dimer. The far- and near-UV circular dichroism spectra of the derivatives were quite similar to those of the intact one. These results indicate that the 23 COOH-terminal amino acids at least are not essential for achieving the full antiviral activity and constructing the higher structure of human IFN-gamma.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sakaguchi M,Honda S,Ozawa M,Nishimura Odoi
10.1016/0014-5793(88)80671-9subject
Has Abstractpub_date
1988-03-28 00:00:00pages
201-4issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(88)80671-9journal_volume
230pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The requirement of divalent cations for binding of the 46 kDa mannose 6-phosphate receptors to phosphomannan and pentamannose 6-phosphate-substituted bovine serum albumin was examined. Receptors from human liver and human brain bound to both affinity ligands in the absence or presence of divalent cations with similar ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80176-5
更新日期:1988-09-12 00:00:00
abstract::DNA synthesis at primers d(pT)n, d(pA)n, d(pC)n, and d(pG)n in the presence of corresponding complementary templates and at hetero-oligoprimers complementary to M13 phage DNA was investigated. The values of both -log Km and log Vmax increased linearly if homo-oligoprimers contained less than 10 nucleotides. The length...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81642-4
更新日期:1989-11-20 00:00:00
abstract::Cyclooxygenases (COXs) are key enzymes in the conversion of arachidonic acid into prostanoids which are involved in apoptosis and inflammation. Two distinct COXs have been identified: COX-1 which is constitutively expressed and COX-2 which is induced by different products such as tumor promoters or growth factors. Pre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02924-6
更新日期:2001-10-12 00:00:00
abstract::gamma-Thrombin stimulated release of [3H]arachidonic acid ([3H]AA) accompanied by a significant production of PAF and lyso-PAF by rabbit platelets. These responses, which reflect PLA2 activation, were observed after a prolonged lag and to a lower extent when compared to those induced by alpha-thrombin which evoked a m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81142-1
更新日期:1989-09-25 00:00:00
abstract::Some members of the Pax (paired box) and Six (sine oculis homeobox) gene families function as components of a gene regulatory network controlling eye development. To investigate the early evolution of the genetic interaction between Pax and Six genes, we identified and sequenced members of the Pax and Six gene familie...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.03.027
更新日期:2007-04-17 00:00:00
abstract::Damage to the central nervous system triggers rapid activation and specific migration of glial cells towards the lesion site. There, glial cells contribute heavily to secondary neuronal changes that take place after lesion. In an attempt to identify the molecular cues of glial activation following brain trauma we perf...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00244-3
更新日期:2004-04-09 00:00:00
abstract::Electrospray/mass spectrometry (ES/MS) was extensively used to obtain information on disulphide-containing intermediates formed during refolding of bovine pancreatic ribonuclease A. The analysis showed the existence of an equilibrated population of disulphide bonded intermediates, and indicates that intermediates cont...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00966-x
更新日期:1994-10-03 00:00:00
abstract::Protein kinase A (PKA) signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi. Employing liquid chromatography-tandem mass spectroscopy, we identified novel phosphorylation sites on the regulatory subu...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12886
更新日期:2017-11-01 00:00:00
abstract::We investigated the effect of hydroxyl substituted chalcone (1a) and some chalcone analogues (1b-d) on isolated rat liver mitochondria to gain new insights into the cytotoxic mechanism of these compounds. We observed an inhibitory effect on phosphorylation and the partial uncoupling of compounds 1a and 1d. Increased r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.11.098
更新日期:2010-02-05 00:00:00
abstract::Oil crops are in increasing demand both for food and as renewable sources of chemicals. It is therefore vital to understand how oil accumulation is regulated. Different ways of obtaining such information are discussed with an emphasis on metabolic control analysis. The usefulness of the latter has been well-illustrate...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.05.018
更新日期:2013-06-27 00:00:00
abstract::Mild sonication of eukaryotic cells produces native plasma membrane sheets that retain their docked organelles, cytoskeleton structures and cytoplasmic complexes. While the delicate organization of membranous protein complexes remains undisturbed, their inner plasmalemmel leaflet can be rapidly exposed to bathing solu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.08.040
更新日期:2008-10-15 00:00:00
abstract::A cDNA sequence putatively encoding a Delta(6)-fatty acid desaturase was isolated from Rhizopus arrhizus using reverse transcription polymerase chain reaction and rapid amplification of cDNA ends methods. Sequence analysis indicated that this cDNA sequence had an open reading frame of 1377 bp encoding 458 amino acids ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01380-2
更新日期:2004-01-02 00:00:00
abstract::Human prothymosin alpha mutants were generated with the aid of random mutagenesis and screened for their ability to inhibit yeast Saccharomyces cerevisiae cell growth. Conversion of Lys-87 to Glu resulted in an inactivated prothymosin alpha mutant, which lost the ability of the wild-type protein to block yeast cell gr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01171-4
更新日期:1996-11-18 00:00:00
abstract::We synthesized and evaluated by surface plasmon resonance 64 LNA/2'-O-methyl sequences corresponding to all possible combinations of such residues in a kissing aptamer loop complementary to the 6-nt loop of the TAR element of HIV-1. Three combinations of LNA/2'-O-methyl nucleoside analogues where one or two LNA units ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.01.047
更新日期:2007-02-20 00:00:00
abstract::Epidermal growth factor (EGF) dose-dependently enhanced the induction of tyrosine aminotransferase and tryptophan oxygenase by glucocorticoids in primary cultures of adult rat hepatocytes without itself having any effect on these enzymes in the absence of glucocorticoids. The amplifications were observed even with dex...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80293-4
更新日期:1987-11-02 00:00:00
abstract::Antimycin, 2-nonyl-4-hydroxyquinoline N-oxide and funiculosin induce O.2(-) generation by submitochondrial particles oxidizing succinate, whereas KCN, mucidin, myxothiazol or 2,3-dimercaptopropanol inhibit O.2(-) generation. Thenoyltrifluoroacetone does not induce superoxide production by itself but slightly stimulate...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80200-2
更新日期:1983-05-02 00:00:00
abstract::The Pb, Eu and Mg-induced cleavages in E. coli and lupine tRNAPhe have been characterized and compared with those found in yeast tRNAPhe. The pattern of lupine tRNAPhe hydrolysis closely resembles that of yeast tRNAPhe, while several major differences occur in the specificity and efficiency of the E. coli tRNAPhe hydr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80148-6
更新日期:1989-01-30 00:00:00
abstract::SARS 8b is one of the putative accessory proteins of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) with unknown functions. In this study, the cellular localization and activity of this estimated 9.6 kDa protein were examined. Confocal microscopy results indicated that SARS 8b is localized in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.05.051
更新日期:2006-06-26 00:00:00
abstract::The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S. cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH. The fo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01016-9
更新日期:1999-08-20 00:00:00
abstract::The selectively red excited emission spectrum, at room temperature, of the in vitro reconstituted Lhca4, has a pronounced non-equilibrium distribution, leading to enhanced emission from the directly excited low-energy pigments. Two different emitting forms (or states), with maximal emission at 713 and 735nm (F713 and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.02.057
更新日期:2005-04-11 00:00:00
abstract::The internalisation of metabotropic glutamate receptor (mGluR1alpha) and its splice variant (mGluR1beta), in response to agonist and phorbol esters (PMA), has been studied. Both mGluR1alpha and mGluR1beta exhibit a similar rate of internalisation following PMA treatment, with a shift in their distribution from plasma ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01353-7
更新日期:1997-11-24 00:00:00
abstract::The complete amino acid sequence of a mammalian acetylcholinesterase from fetal bovine serum (FBS AChE) is presented. This enzyme has a high degree of sequence identity with other cholinesterases, liver carboxyesterases, esterase-6, lysophospholipase, and thyroglobulin. The locations of 191 amino acids in 10 regions o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81522-p
更新日期:1990-06-18 00:00:00
abstract::Here, we investigated the molecular mechanism underlying the changes in the distribution of nucleolin. Our study identified PI3K/Akt signaling as an essential pathway regulating the distribution of nucleolin. Furthermore, nucleolin can interact with phospho-PI3K-p55, and changes in the distribution of nucleolin were r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.03.047
更新日期:2014-05-21 00:00:00
abstract::Irreversible conversion of the purified zinc-containing 7Fe ferredoxin from the thermoacidophilic archaeon Sulfolobus sp. strain 7 was found to occur under aerobic conditions at pH 5.0 and at 4 degrees C. This process accompanied a substantial increase of the electron paramagnetic resonance signal attributed to a [3Fe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01286-6
更新日期:1997-11-10 00:00:00
abstract::Focal adhesion (FA) proteins, kindlin-2 and integrin-linked kinase (ILK), regulate cell adhesion and migration. ILK interacts with and promotes kindlin-2 targeting to FAs. Leu353 and Leu357 in kindlin-2 have been reported to be important for the interaction between kindlin-2 and ILK. However, the binding interface bet...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12938
更新日期:2018-01-01 00:00:00
abstract::Reconstituted Na+,K+-ATPase from either pig kidney or shark rectal glands was phosphorylated by cAMP dependent protein kinase, PKA. The stoichiometry was approximately 0.9 mol P(i)/mol alpha-subunit in the pig kidney enzyme and approximately 0.2 mol P(i)/mol alpha-subunit in the shark enzyme. In shark, Na+,K+-ATPase P...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00032-4
更新日期:1996-02-19 00:00:00
abstract::Annexins are multifunctional intracellular proteins with Ca2+- and phospholipid-binding properties. Their structures consist of four conserved repeat domains that form the core and a diverse N-terminal tail, from which their functional differences may arise. We searched for cellular proteins that interact with the N-t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01643-3
更新日期:1999-01-15 00:00:00
abstract::The efficiency of visible light in inducing fluorescence quenching in the isolated light-harvesting complex II (LHCII) of higher plants is investigated by action spectroscopy in the visible portion of photosynthetic active radiation. The efficiency spectrum displays a relatively homogenous quenching yield across the m...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12279
更新日期:2016-08-01 00:00:00
abstract::Placental alkaline phosphatase is an inducible enzyme, expressed in HeLaS3 cells, which has been shown to possess protein phosphotyrosine phosphatase activity. Since phosphotyrosine levels are known to increase in actively dividing cells we sought an inverse correlation between PLAP activity and growth rate in HeLaS3 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80229-n
更新日期:1993-08-30 00:00:00
abstract::The Wnt/beta-catenin signalling pathway is evolutionarily conserved across many species and plays important roles during embryogenesis. The Lee-Heinrich model (to recognize the contributions of R. Heinrich we will refer to the work proposed by Lee et al. [Lee, E., Salic, A., Krüger, R., Heinrich, R., Kirschner, M.W. (...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.07.043
更新日期:2007-08-21 00:00:00