Calcium-dependent interaction of annexin I with annexin II and mapping of the interaction sites.

abstract：:The structure of [Val1]gramicidin A incorporated into sodium dodecyl-d25 sulphate micelles has been studied by two-dimensional proton NMR spectroscopy. Analysis of nuclear Overhauser effects, spin-spin couplings and solvent accessibility of NH groups show that the conformation of the Na+ complex of gramicidin A in det...

journal_title：FEBS letters

authors： Arseniev AS,Barsukov IL,Bystrov VF,Lomize AL,Ovchinnikov YuA

更新日期：1985-07-08 00:00:00

abstract：:Using radiolysis with (32)P enriched phosphate as an internal source of ionizing radiation, the formation of hydroperoxo-ferric complex from oxy-ferrous precursor with a high yield was monitored at 77 K in heme oxygenase (HO) by means of optical absorption spectroscopy. Well-resolved absorption spectra (maxima at 421 ...

journal_title：FEBS letters

authors： Denisov IG,Ikeda-Saito M,Yoshida T,Sligar SG

更新日期：2002-12-04 00:00:00

abstract：:Foot-and-mouth disease virus leader proteinase (L(pro)), a papain-like cysteine proteinase, has six acidic amino acids between 4 A and 11 A of the catalytic dyad of Cys51 and His148. In contrast, in papain and related enzymes, only one acidic residue lies within this distance. We have examined by site-directed mutagen...

journal_title：FEBS letters

authors： Kronovetr J,Skern T

更新日期：2002-09-25 00:00:00

abstract：:Protein kinases play a crucial role in the regulation of cellular processes. Most eukaryotes reserve about 2.5% of their genes for protein kinases. We analysed the genome of the single-celled ciliate Paramecium tetraurelia and identified 2606 kinases, about 6.6% of its genes, representing the largest kinome to date. A...

journal_title：FEBS letters

authors： Bemm F,Schwarz R,Förster F,Schultz J

更新日期：2009-11-19 00:00:00

abstract：:The Surfeit locus is an unusual cluster of at least 6 housekeeping genes whose organisation is conserved between birds and mammals. We have previously shown that the divergently transcribed Surf-1 and Surf-2 genes are separated by a bi-directional, TATA-less promoter. In mouse, the Surf-1/Surf-2 promoter contains thre...

journal_title：FEBS letters

authors： Gaston K,Fried M

更新日期：1994-06-27 00:00:00

abstract：:African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demo...

journal_title：FEBS letters

authors： Matsuo E,Celma CC,Roy P

更新日期：2010-08-04 00:00:00

abstract：:Tumor necrosis factor TNF can trigger increases in membrane conductance of mammalian cells in a receptor-independent manner via its lectin-like domain. A lectin-deficient TNF mutant, lacking this activity, was able to bind to artificial liposomes in a pH-dependent manner, but not to insert into the bilayer, just like ...

journal_title：FEBS letters

authors： van der Goot FG,Pugin J,Hribar M,Fransen L,Dunant Y,De Baetselier P,Bloc A,Lucas R

更新日期：1999-10-22 00:00:00

abstract：:Granulocyte colony-stimulating factor (G-CSF) has been recently shown to induce the high-affinity Fc receptor for IgG (Fc(gammaRI)/CD64) in human polymorphonuclear neutrophils (PMN). To elucidate the molecular mechanisms whereby G-CSF exerts this effect, we examined whether the cytokine induces the binding of transcri...

journal_title：FEBS letters

authors： Bovolenta C,Gasperini S,Cassatella MA

更新日期：1996-05-20 00:00:00

abstract：:We report a nonenzymatic reaction which results in the radiolabeling of proteins by 32P-containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modificati...

journal_title：FEBS letters

authors： Schmidt MC,Hanna MM

更新日期：1986-01-06 00:00:00

abstract：:The binding of folate to Lactobacillus casei dihydrofolate reductase in the presence and absence of NADP+ has been studied by 15N NMR, using [5-15N]folate. In the presence of NADP+, three separate signals were observed for the single 15N atom, in agreement with our earlier evidence from 1H and 13C NMR for multiple con...

journal_title：FEBS letters

authors： Birdsall B,De Graw J,Feeney J,Hammond S,Searle MS,Roberts GC,Colwell WT,Crase J

更新日期：1987-06-08 00:00:00

abstract：:Anandamide loses its cannabimimetic activities upon hydrolysis to arachidonic acid and ethanolamine. So far the anandamide hydrolyzing activity widely distributed in mammalian organs has been attributed exclusively to an enzyme referred to as anandamide amidohydrolase with an optimum pH around 9. We found another enzy...

journal_title：FEBS letters

authors： Ueda N,Yamanaka K,Terasawa Y,Yamamoto S

更新日期：1999-07-09 00:00:00

abstract：:Monocyte chemoattractant protein-1 (MCP-1) is a member of the beta chemokine family which acts through specific seven transmembrane receptors to recruit monocytes, basophils, and T lymphocytes to sites of inflammation. To identify regions of the human MCP-1 protein which are important for its biological activity, we h...

journal_title：FEBS letters

authors： Steitz SA,Hasegawa K,Chiang SL,Cobb RR,Castro MA,Lobl TJ,Yamada M,Lazarides E,Cardarelli PM

更新日期：1998-07-03 00:00:00

abstract：:Long-chain fatty acid amides are cell-signaling lipids identified in mammals and, recently, in invertebrates, as well. Many details regarding fatty acid amide metabolism remain unclear. Herein, we demonstrate that Drosophila melanogaster is an excellent model system for the study long-chain fatty acid amide metabolism...

journal_title：FEBS letters

authors： Jeffries KA,Dempsey DR,Behari AL,Anderson RL,Merkler DJ

更新日期：2014-05-02 00:00:00

abstract：:We have reconstituted thin filaments comprising pyrene-labelled actin (pyr-actin), tropomyosin (Tm) and cardiac troponin (cTn). cTn was isolated in two defined phosphorylation states; completely dephosphorylated on all subunits and with only the cTnI subunit bisphosphorylated. The thin filament was saturated with cTn ...

journal_title：FEBS letters

authors： Reiffert SU,Jaquet K,Heilmeyer LM Jr,Ritchie MD,Geeves MA

更新日期：1996-04-08 00:00:00

abstract：:The MAP kinase module (Raf/MAPKKK-MAPKK-MAPK) has been shown to be sequentially activated after mitogenic stimulation. Here we demonstrate, by site directed mutagenesis, that MAPK is able to retrophosphorylate its own activator, MAPKK, on two threonine residues Thr-292 and Thr-386 in vitro, and that these sites are al...

journal_title：FEBS letters

authors： Brunet A,Pagès G,Pouysségur J

更新日期：1994-06-13 00:00:00

abstract：:Microdissected, beta-cell-rich pancreatic islets from ob/ob mice were used in studies of 86Rb+ transport. D-Glucose (20 mM) induced a biphasic reduction in 86Rb+ efflux. The reduction stabilized within 10 min at 34% of the efflux rate at zero glucose. The initial 86Rb+ uptake (5 min) was dose-dependently reduced by ou...

journal_title：FEBS letters

authors： Lindström P,Norlund L,Sehlin J

更新日期：1986-05-05 00:00:00

abstract：:To elucidate hydroxyapatite-protein interaction, mutant human lysozymes in which the surface charge was modified by site-directed mutagenesis were used. Five mutant human lysozymes (K1A, K13A, K33A, R10A, R14A) were expressed in yeast. The chromatographic behavior of these lysozymes was studied with a HPLC hydroxyapat...

journal_title：FEBS letters

authors： Aizawa T,Koganesawa N,Kamakura A,Masaki K,Matsuura A,Nagadome H,Terada Y,Kawano K,Nitta K

更新日期：1998-01-30 00:00:00

abstract：:Cyanomorpholinoadriamycin (1 microM) was reacted in a transcription buffer with DNA of an initiated transcription complex. Subsequent elongation of the initiated complex revealed permanent transcriptional blockages at 16 sites after only 5 min of drug-DNA reaction time. The most dominant sites were immediately prior t...

journal_title：FEBS letters

authors： Cullinane C,Phillips DR

更新日期：1991-11-18 00:00:00

abstract：:During the course of large scale purification of the D1 dopamine receptor from rat brain, a protein of approximately 87,000 daltons (p87) was observed to copurify with the D1 receptor through four chromatographic steps. To characterize the nature of this protein, bovine and rat cDNA clones were isolated and sequenced....

journal_title：FEBS letters

authors： Gingrich JA,Andersen PH,Tiberi M,el Mestikawy S,Jorgensen PN,Fremeau RT Jr,Caron MG

更新日期：1992-11-09 00:00:00

abstract：:A-to-I RNA editing can lead to recoding of pre-mRNAs with profound functional consequences for the ensuing proteins. Here we show that complement component 1, q subcomponent-like 1 (C1QL1) undergoes RNA editing in vivo causing non-synonymous amino acid substitutions in human, mouse as well as zebrafish. The major edit...

journal_title：FEBS letters

authors： Sie CP,Maas S

更新日期：2009-04-02 00:00:00

abstract：:The WRN protein is mutated in the chromosomally unstable Werner syndrome (WS) and the Nbs1 protein is mutated in Nijmegen breakage syndrome (NBS). The Nbs1 protein is an integral component of the M/R/N complex. Although WRN is known to interact with this complex in response to gamma-irradiation, the mechanism of actio...

journal_title：FEBS letters

authors： Cheng WH,Sakamoto S,Fox JT,Komatsu K,Carney J,Bohr VA

更新日期：2005-02-28 00:00:00

abstract：:Isolated rat islets of Langerhans permeabilised by high-voltage discharge secreted insulin in response to elevations in Ca2+ over the range 100 nM to 10 microM Ca2+. The phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA), had no effects on insulin secretion in the absence of Ca2+. In the presence of Ca2+ concen...

journal_title：FEBS letters

authors： Jones PM,Stutchfield J,Howell SL

更新日期：1985-10-21 00:00:00

abstract：:Coronin proteins are widely expressed among eukaryotic organisms. Most coronins consist of a WD-repeat domain followed by a C-terminal coiled coil. Dictyostelium discoideum expresses a single short coronin coronin A, which has been implicated in both actin modulation and multicellular differentiation. Whether coronin ...

journal_title：FEBS letters

authors： Fiedler T,Fabrice TN,Studer V,Vinet A,Faltova L,Kammerer RA,Steinmetz MO,Sharpe T,Pieters J

更新日期：2020-04-16 00:00:00

abstract：:H2O2 addition to the oxidized cytochrome c oxidase reconstituted in liposomes brings about a red shift of the Soret band of the enzyme and an increased absorption in the visible region with two distinct peaks at approximately 570 and 605 nm. Throughout pH range 6-8.5, the spectral changes at 570 nm and in the Soret ba...

journal_title：FEBS letters

authors： Vygodina T,Konstantinov AA

更新日期：1987-07-27 00:00:00

abstract：:Folding of nascent chains resembles the decoding of spoken language in that information is emitted as a unidirectional, one-dimensional string of elements, with higher structures and long-distance interactions emerging with time. Applying a "pseudolinguistic' analysis of structure to a set of all 36 possible six-stran...

journal_title：FEBS letters

authors： Gross M

更新日期：1996-07-29 00:00:00

abstract：:Mutations in the nuclear matrix protein Matrin 3 (MATR3) have been identified in amyotrophic lateral sclerosis and myopathy. To investigate the mechanisms underlying MATR3 mutations in neuromuscular diseases and efficiently screen for modifiers of MATR3 toxicity, we generated transgenic MATR3 flies. Our findings indic...

journal_title：FEBS letters

authors： Zhao M,Kao CS,Arndt C,Tran DD,Cho WI,Maksimovic K,Chen XXL,Khan M,Zhu H,Qiao J,Peng K,Hong J,Xu J,Kim D,Kim JR,Lee J,van Bruggen R,Yoon WH,Park J

更新日期：2020-09-01 00:00:00

abstract：:Phosphatidylglycerophosphate (PGP) synthase, encoded by PGP1 and PGP2 in Arabidopsis, catalyzes a committed step in the biosynthesis of phosphatidylglycerol (PG). In this study, we isolated a pgp1pgp2 double mutant of Arabidopsis to study the function of PG. In this mutant, embryo development was delayed and the major...

journal_title：FEBS letters

authors： Tanoue R,Kobayashi M,Katayama K,Nagata N,Wada H

更新日期：2014-05-02 00:00:00

abstract：:We have isolated a 1785-bp complementary DNA (cDNA) encoding the murine P2X7 receptor subunit from NTW8 mouse microglial cells. The encoded protein has 80%, and 85% homology to the human and rat P2X7 subunits, respectively. Functional properties of the heterologously expressed murine P2X7 homomeric receptor broadly re...

journal_title：FEBS letters

authors： Chessell IP,Simon J,Hibell AD,Michel AD,Barnard EA,Humphrey PP

更新日期：1998-11-13 00:00:00

abstract：:A selective PKC inhibitor, UCN-01, was shown to exhibit anti-tumor activity in vitro and in vivo. We investigated UCN-01 with respect to isozyme-specific PKC inhibition using purified recombinant or rabbit brain PKC isozymes, cPKC alpha, beta and gamma, nPKC delta, epsilon and eta, and a PKC zeta. Of the PKC isozymes ...

journal_title：FEBS letters

authors： Mizuno K,Noda K,Ueda Y,Hanaki H,Saido TC,Ikuta T,Kuroki T,Tamaoki T,Hirai S,Osada S

更新日期：1995-02-13 00:00:00

abstract：:Ceramide-1-phosphate (CER-1-P) is emerging as a novel bioactive sphingolipid. It is formed by phosphorylation of ceramide catalyzed by ceramide kinase, and has been implicated in different cellular processes. Cer-1-P is mitogenic for fibroblasts, blocks apoptosis in macrophages, controls phagocytosis in neutrophils, a...

journal_title：FEBS letters

authors： Gómez-Muñoz A

更新日期：2004-03-26 00:00:00