Abstract:
:Hepatitis C Virus core protein (HCVc) plays important roles in the development of intrahepatic cholangiocarcinoma (ICC). MicroRNAs (miRNAs) contribute to tumor progression by interacting with downstream target genes. However, the regulation and role of miRNAs in HCV-related intrahepatic cholangiocarcinoma (HCV-ICC) is poorly understood. In this study, we found that miR-124 was down-regulated in HCV-ICC and the induction of DNMT1 by HCVc mediated the suppression of miR-124. Over-expression of miR-124 suppressed cell migration and invasion in vitro, and reduced the protein levels of SMYD3 and downstream target genes (c-Myc and MMP9). Knockdown of SMYD3 inhibited cell migration and invasion resembling that of miR-124 over-expression. In conclusion, our studies indicate that low miR-124 levels mediated by HCVc via DNMT1 promote ICC cell migration and invasion by targeting SMYD3.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zeng B,Li Z,Chen R,Guo N,Zhou J,Zhou Q,Lin Q,Cheng D,Liao Q,Zheng L,Gong Ydoi
10.1016/j.febslet.2012.06.049subject
Has Abstractpub_date
2012-09-21 00:00:00pages
3271-8issue
19eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00539-Xjournal_volume
586pub_type
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