Abstract:
:Dendritic cells (DCs) are known to modulate immune response by activating effector cells of both the innate and the adaptive immune system. In the present study, we demonstrate that co-culture of DCs with paraformaldehyde-fixed tumor cells augments the secretion of interleukin (IL)-12 by DCs and these activated DCs upon co-culture with naive NK cells enhance the cytolytic activity of NK cells against NK-sensitive target YAC-1. Similarly, DCs isolated from tumor-bearing animals also activated NK cells in vitro. For efficient activation of NK cells, the ratio of activated DCs to NK cells is crucial. Addition of anti-IL-12 antibody to the culture system completely abolished activation of NK cells by DCs, suggesting that IL-12 secreted by DCs is an essential factor in NK cell activation. Adoptive transfer of DCs isolated from tumor-bearing animals into normal rats also induced activation of NK cells in normal animals.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Alli RS,Khar Adoi
10.1016/S0014-5793(04)00026-2keywords:
subject
Has Abstractpub_date
2004-02-13 00:00:00pages
71-6issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579304000262journal_volume
559pub_type
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