Abstract:
:We investigated here whether adiponectin can exhibit an inhibitory effect on tumor necrosis factor-alpha (TNF-alpha)- and receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclastogenesis by using RAW264 cell D clone with a high efficiency to form osteoclasts. Globular adiponectin (gAd) strongly inhibited TNF-alpha/RANKL-induced differentiation of osteoclasts by interfering with TNF receptor-associated factor 6 production and calcium signaling; consequently, the induction of nuclear factor of activated T cells c1 (NFATc1) was strongly inhibited. Moreover, we observed that inhibition of AMP-activated protein kinase abrogated gAd inhibition for TNF-alpha/RANKL-induced NFATc1 expression. Our data suggest that adiponectin acts as a potent regulator of bone resorption observed in diseases associated with cytokine activation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yamaguchi N,Kukita T,Li YJ,Kamio N,Fukumoto S,Nonaka K,Ninomiya Y,Hanazawa S,Yamashita Ydoi
10.1016/j.febslet.2007.12.037subject
Has Abstractpub_date
2008-02-06 00:00:00pages
451-6issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(08)00014-8journal_volume
582pub_type
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