Abstract:
:Locus control regions (LCRs) are cis-acting elements that confer position-independent and copy-number-dependent expression upon associated genes in transgenic mice. Here we show the second example of the use of an LCR (the rat LAP LCR) in a stable expression vector system, used here in conjunction with the rat liver (NRLM) cell line. Non-transfected NRLM cells are electrically silent and highly suitable for patch clamp electrophysiology. We report reliable constitutive expression from two different K+ channel cDNAs; the voltage-gated rat clone Kv3.4 and the inward rectifier mouse clone Kir2.1. We further show that constitutive expression levels are stable for at least 8 weeks from initial recording.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Shelton PA,Dart Cdoi
10.1016/0014-5793(96)00755-7subject
Has Abstractpub_date
1996-08-12 00:00:00pages
282-6issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(96)00755-7journal_volume
391pub_type
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