Cysteine is not a substrate but a specific modulator of human ASCT2 (SLC1A5) transporter.


:The Alanine Serine Cysteine Transporter 2 (ASCT2) is involved in balancing the intracellular amino acid pool. This function is allowed by the antiport mechanism and the asymmetric specificity towards different neutral amino acids, distinctive of this transporter. In the present work, the interaction of the putative substrate Cys with the human ASCT2 has been studied using the recombinant hASCT2 over-produced in Pichia pastoris and the native ASCT2 extracted from HeLa in both proteoliposomes and intact cells. It was found that Cys is a potent competitive inhibitor of hASCT2 but is not a substrate. Moreover, Cys binding to a second site, different from that of substrate, triggers a protein-mediated unidirectional Gln efflux.




FEBS letters


Scalise M,Pochini L,Pingitore P,Hedfalk K,Indiveri C




Has Abstract


2015-11-30 00:00:00














  • N-terminal amino acid sequences of precursor and mature forms of alpha-1-antitrypsin.

    abstract::alpha-1-Antitrypsin is found in hepatocytes as a high-mannose glycoprotein (Mr 49 000), extracellularly as a complex-type glycoprotein (Mr 54 000). Deglycosylation of both forms with peptide: N-glycosidase led to proteins of identical app. Mr (41 000). The sequence of 26 N-terminal amino acids of rat alpha 1-antitryps...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gross V,Kaiser C,Tran-Thi TA,Schmelzer E,Witt I,Plummer TH Jr,Heinrich PC

    更新日期:1983-01-24 00:00:00

  • The effect of local anaesthetics on the ryanodine receptor/Ca2+ release channel of brain microsomal membranes.

    abstract::The effects of various local anaesthetics (LAs) on ryanodine binding of the sheep brain ryanodine receptor were tested. Tetracaine and dibucaine inhibit the binding with half-maximal inhibition (CI50) of 0.12 mM and 0.7 mM, respectively. Lidocaine and its analog QX-314, on the other hand, stimulate the binding up to 3...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Martin C,Ashley R,Shoshan-Barmatz V

    更新日期:1993-08-09 00:00:00

  • Arrestin from nucleated red blood cells binds to bovine rhodopsin in a light-dependent manner.

    abstract::Using a panel of monoclonal antibodies, it has previously been demonstrated that the cytosol of nucleated red cells (trout and turkey) contains a protein similar to arrestin, a soluble protein found so far only in the photosensitive cells and which, by binding to photoexcited rhodopsin, inhibits the phototransduction ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Scheuring U,Franco M,Fievet B,Guizouarn H,Mirshahi M,Faure JP,Motais R

    更新日期:1990-12-10 00:00:00

  • Characterization of the c-specific promoter of the gene encoding human endothelin-converting enzyme-1 (ECE-1).

    abstract::Human ECE-1 is expressed in four isoforms with different tissue distribution and its mRNA and protein levels are altered under certain pathophysiological conditions. To investigate the transcriptional regulation of ECE-1, we studied the regulatory region of ECE-1c, the major ECE-1 isoform. A genomic clone comprising t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Funke-Kaiser H,Bolbrinker J,Theis S,Lemmer J,Richter CM,Paul M,Orzechowski HD

    更新日期:2000-01-28 00:00:00

  • Isolation and characterisation of a functional alpha beta heterodimer from the ATP synthase of Rhodospirillum rubrum.

    abstract::An alpha beta heterodimer of the F1-ATPase of Rhodospirillum rubrum was isolated by extraction of chromatophores with LiCl. Each alpha beta heterodimer contains one tightly bound ADP, which is released upon removal of medium Mg2+. The dimer can be reversibly dissociated by removal of Mg(2+)-ions. The alpha beta hetero...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Andralojc PJ,Harris DA

    更新日期:1992-09-28 00:00:00

  • Bicarbonate ions and pH regulation of Leishmania major promastigotes.

    abstract::Leishmania major promastigotes are parasites endowed with a plasma membrane electrogenic H+ pump and anionic channels. These systems have been thought to contribute to pH homeostasis of parasites and environmental adaptation by mediating extrusion of protons which are either generated metabolically or result from exog...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vieira L,Cabantchik ZI

    更新日期:1995-03-13 00:00:00

  • Perinuclear localization of cytosolic phospholipase A(2)alpha is important but not obligatory for coupling with cyclooxygenases.

    abstract::In response to Ca(2+) signaling, cytosolic phospholipase A(2)alpha (cPLA(2)alpha) translocates from the cytosol to the perinuclear membrane, where downstream eicosanoid-synthetic enzymes, such as cyclooxygenase (COX), are localized. Although the spatiotemporal perinuclear colocalization of cPLA(2)alpha and COXs has be...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Murakami M,Das S,Kim YJ,Cho W,Kudo I

    更新日期:2003-07-10 00:00:00

  • N-linked glycosylation of platelet P2Y12 ADP receptor is essential for signal transduction but not for ligand binding or cell surface expression.

    abstract::P(2)Y(12) receptor is a G(i)-coupled adenosine diphosphate (ADP) receptor with a critical role in platelet aggregation. It contains two potential N-linked glycosylation sites at its extra cellular amino-terminus, which may modulate its activity. Studies of both tunicamycin treatment and site-directed mutagenesis have ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhong X,Kriz R,Seehra J,Kumar R

    更新日期:2004-03-26 00:00:00

  • The amphiphilic character of glycogenin.

    abstract::This study describes for the first time the amphiphilicity of the protein moiety of proteoglycogen. Glycogenin but not proteoglycogen associates to phospholipid vesicles and forms by itself stable Gibbs and Langmuir monolayers at the air-buffer interface. The adsorption free energy (-6.7 kcal/mol) and the glycogenin c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Carrizo ME,Miozzo MC,Maggio B,Curtino JA

    更新日期:2001-12-07 00:00:00

  • Inhibition of Moloney murine leukemia virus replication by tyrphostins, tyrosine kinase inhibitors.

    abstract::We have previously shown that topoisomerase I (topo I) antagonist inhibited retrovirus replication. Since tyrphostins, synthetic compounds and protein tyrosine kinases (PTKs) blockers, inhibited topo I activity (manuscript in preparation) we examined their ability to inhibit Moloney murine leukemia virus (Mo-MuLV) rep...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Esther A,Iftach S,Esther P

    更新日期:1994-03-14 00:00:00

  • Cytoplasmic pH, a key determinant of growth factor-induced DNA synthesis in quiescent fibroblasts.

    abstract::In response to growth factors, quiescent fibroblast mutants lacking Na+/H+ exchange activity fail to elevate their cytoplasmic pH (pHi) and to reinitiate DNA synthesis at neutral and acidic pHo. A pHi threshold of approximately 7.2 exists, below which growth factors cannot set in motion the Go to S phase transition. R...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pouysségur J,Franchi A,L'Allemain G,Paris S

    更新日期:1985-10-07 00:00:00

  • Kinetic studies of the active sites functioning in the quinohemoprotein fructose dehydrogenase.

    abstract::Steady-state kinetic analysis was performed on the reaction between D-fructose and ferricyanide with the quinohemoprotein fructose dehydrogenase from Gluconobacter species. The D-fructose oxidation dependence on the ferricyanide concentration resulted in a series of parallel reciprocal plots, and the reaction was assu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Marcinkeviciene J,Johansson G

    更新日期:1993-02-22 00:00:00

  • 2-Cyclopenten-1-one and prostaglandin J2 reduce restenosis after balloon angioplasty in rats: role of NF-kappaB.

    abstract::The aim of this study was to evaluate, using a rat model of balloon angioplasty, whether prostaglandin (PG) J(2) and 2-cyclopenten-1-one are able to reduce restenosis. We found that both PGJ(2) and 2-cyclopenten-1-one, administered by local application on carotid arteries, caused a dose-dependent inhibition of neointi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ianaro A,Maffia P,Cuzzocrea S,Mazzon E,Santoro MG,Di Rosa M,Ialenti A

    更新日期:2003-10-09 00:00:00

  • Nicking of the tryptophan synthase beta 2-subunit at Glu-296 prevents the conformational change undergone on binding the alpha-subunit.

    abstract::Using a monoclonal antibody as conformational probe it has been shown that the weakly active nicked-beta 2 dimer of tryptophan synthase generated by proteolytic cleavage at Glu-296, does not undergo on association with alpha subunit a conformational change known to occur in intact beta 2 subunit. This alpha induced co...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Linkens HJ,Djavadi-Ohaniance L,Goldberg ME

    更新日期:1993-04-12 00:00:00

  • Insulin-responsive glucose transporters are concentrated in a cell surface-derived membrane fraction of 3T3-L1 adipocytes.

    abstract::The recently proposed mechanistic concept of a receptor-regulated entrance compartment for hexose transport formed by microvilli on 3T3-L1 adipocytes predicted a preferential localization of glucose transporters in these structures. The cytochalasin B-binding technique was used to determine in basal and insulin-stimul...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lange K,Brandt U

    更新日期:1990-02-26 00:00:00

  • Interaction between Sox proteins of two physiologically distinct bacteria and a new protein involved in thiosulfate oxidation.

    abstract::Organisms using the thiosulfate-oxidizing Sox enzyme system fall into two groups: group 1 forms sulfur globules as intermediates (Allochromatium vinosum), group 2 does not (Paracoccus pantotrophus). While several components of their Sox systems are quite similar, i.e. the proteins SoxXA, SoxYZ and SoxB, they differ by...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Welte C,Hafner S,Krätzer C,Quentmeier A,Friedrich CG,Dahl C

    更新日期:2009-04-17 00:00:00

  • Generation of Gla-domainless FVIIa by cathepsin G-mediated cleavage.

    abstract::Coagulation factor VII contains ten gamma-carboxyglutamic acid residues in the N-terminal region (Gla-domain) which are essential for the hemostatic function of FVII. The present study shows that granulocyte cathepsin G degrades the Gla-domain of FVIIa in vitro. Characterization of the truncated FVIIa by SDS-PAGE and ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nicolaisen EM,Petersen LC,Thim L,Jacobsen JK,Christensen M,Hedner U

    更新日期:1992-07-20 00:00:00

  • Actin microridges characterized by laser scanning confocal and atomic force microscopy.

    abstract::We have characterized the cell surface of zebrafish stratified epithelium using a combined approach of light and atomic force microscopy under conditions which simulate wound healing. Microridges rise on average 100 nm above the surface of living epithelial cells, which correlate to bundles of cytochalasin B-insensiti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sharma A,Anderson KI,Müller DJ

    更新日期:2005-03-28 00:00:00

  • Human prion proteins expressed in Escherichia coli and purified by high-affinity column refolding.

    abstract::An efficient method is presented for the production of intact mammalian prion proteins and partial sequences thereof. As an illustration we describe the production of polypeptides comprising residues 23-231, 81-231, 90-231 and 121-231 of the human prion protein (hPrP). Polypeptides were expressed as histidine tail fus...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zahn R,von Schroetter C,Wüthrich K

    更新日期:1997-11-17 00:00:00

  • Links between fatty acids and expression of UCP2 and UCP3 mRNAs.

    abstract::Physiological and pathological states that are associated with elevated plasma fatty acids (FAs) increase uncoupling protein 2 (UCP2) mRNA in white adipose tissue and UCP3 mRNA in skeletal muscle and heart. A direct effect of unsaturated fatty acids from all classes has been shown in various cultured cells. There is e...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Thompson MP,Kim D

    更新日期:2004-06-18 00:00:00

  • A stimulating effect of guanyl nucleotides on the rat-liver soluble cyclic GMP high-affinity phosphodiesterase activity.

    abstract::The high affinity (low Km) cyclic GMP phosphodiesterase (PDE) is activated by GTP, while the cyclic AMP PDE is not. GTP and its hydrolysis-resistant analogue, guanylylimidodiphosphate (GppNHp), display a half-maximal stimulating effect at almost the same concentration (5 X 10(-6) M). The GTP stimulating effect is not ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Spina A,Chiosi E,Paolisso G,Laurenza A,Illiano G

    更新日期:1983-07-04 00:00:00

  • B cell translocation gene, a direct target of miR-142-5p, inhibits vascular smooth muscle cell proliferation by down-regulating cell cycle progression.

    abstract::Vascular smooth muscle cell (VSMC) proliferation plays a key role in neointimal hyperplasia and restenosis. Here we report the role of the microRNA miR-142-5p and its downstream target genes on the proliferation of cultured VSMCs. miR-142-5p promoted VSMC proliferation by down-regulating B cell translocation gene 3 (B...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kee HJ,Park S,Kwon JS,Choe N,Ahn Y,Kook H,Jeong MH

    更新日期:2013-08-02 00:00:00

  • RNA silencing platforms in plants.

    abstract::Since the discovery of RNAi, its mechanism in plants and animals has been intensively studied, widely exploited as a research tool, and used for a number of potential commercial applications. In this article, we discuss the platforms for delivering RNAi in plants. We provide a brief background to these platforms and c...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Watson JM,Fusaro AF,Wang M,Waterhouse PM

    更新日期:2005-10-31 00:00:00

  • The kinetics of the reaction of superoxide radical with Fe(III) complexes of EDTA, DETAPAC and HEDTA.

    abstract::To gain an understanding of the mechanism by which the hydroxyl free radical can arise in superoxide generating systems and learn how different chelaters of iron can inhibit this reaction, a pulse radiolysis kinetic study of the reaction of O;- with Fe(III)EDTA, Fe(III)HEDTA and Fe(III)DETAPAC (or DTPA)was undertaken....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Buettner GR,Doherty TP,Patterson LK

    更新日期:1983-07-11 00:00:00

  • Islet-activating protein prevents nicotinic acid-induced GTPase stimulation and GTP but not GTP gamma S-induced adenylate cyclase inhibition in rat adipocytes.

    abstract::The influence of islet-activating protein (IAP), a Bordetella pertussis toxin, was studied on adenylate cyclase and GTPase activities in rat adipocyte membranes. Pretreatment of rats or intact rat adipocytes with IAP did not affect adenylate cyclase inhibition by the stable GTP analog, GTP gamma S, whereas inhibition ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Aktories K,Schultz G,Jakobs KH

    更新日期:1983-05-30 00:00:00

  • Three-dimensional model of NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase and relationships to the NADP(+)-dependent enzyme (carbonyl reductase).

    abstract::Modelling the amino acid sequence of NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase into the three-dimensional structure of 3 alpha/20 beta-hydroxysteroid dehydrogenase shows that these two enzymes, as well as the NADP(+)-linked prostaglandin dehydrogenase (identical to carbonyl reductase) have similar conformati...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Krook M,Ghosh D,Duax W,Jörnvall H

    更新日期:1993-05-10 00:00:00

  • Spectroelectrochemistry of P700 in native photosystem I particles and diethyl ether-treated thylakoid membranes from spinach and Thermosynechococcus elongatus.

    abstract::The redox potentials (E(composite function')) of P700 in intact and diethyl ether-treated thylakoid membranes as well as native photosystem (PS) I particles from spinach and Thermosynechococcus elongatus have been measured by a spectroelectrochemistry with an error range of +/-2-3 mV. Stepwise removal of antenna pigme...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhang Y,Nakamura A,Kuroiwa Y,Kato Y,Watanabe T

    更新日期:2008-04-02 00:00:00

  • The phosphorylation at Thr 124 of simian virus 40 large T antigen is crucial for its oligomerization.

    abstract::SV40 large T antigen is phosphorylated at up to ten different amino acids clustered in an N-terminal and a C-terminal part of the polypeptide chain. The N-terminal phosphorylated residues include Ser 123 and Thr 124. We have analyzed the oligomerization, the complex formation with the cellular oncoprotein p53 and the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Montenarh M,Müller D

    更新日期:1987-09-14 00:00:00

  • Modulation of EWS/WT1 activity by the v-Src protein tyrosine kinase.

    abstract::Desmoplastic small round cell tumor (DSRCT) is a malignant human cancer that is associated with a specific t(11;22) chromosome translocation, where 265 amino acids from the EWS amino-terminus are fused to the DNA binding domain of the WT1 tumor suppressor gene. We have noticed the presence of several SH3 interacting d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kim J,Lee JM,Branton PE,Pelletier J

    更新日期:2000-06-02 00:00:00

  • Do monoclonal antibodies Tü15 and Tü67 detect heterogeneity of human transferrin receptor molecules?

    abstract::The possible molecular heterogeneity of human transferrin receptors was analyzed using two murine monoclonal antibodies, Tü15 and Tü67. Both reagents precipitated from lysates of 125I-labeled HL-60 cells a major component of 88 kDa which could be identified as the transferrin receptor by comparison with the proteins d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Uchańska-Ziegler B,Wernet P,Liangru S,Ziegler A

    更新日期:1984-10-01 00:00:00