Cysteine is not a substrate but a specific modulator of human ASCT2 (SLC1A5) transporter.

Abstract:

:The Alanine Serine Cysteine Transporter 2 (ASCT2) is involved in balancing the intracellular amino acid pool. This function is allowed by the antiport mechanism and the asymmetric specificity towards different neutral amino acids, distinctive of this transporter. In the present work, the interaction of the putative substrate Cys with the human ASCT2 has been studied using the recombinant hASCT2 over-produced in Pichia pastoris and the native ASCT2 extracted from HeLa in both proteoliposomes and intact cells. It was found that Cys is a potent competitive inhibitor of hASCT2 but is not a substrate. Moreover, Cys binding to a second site, different from that of substrate, triggers a protein-mediated unidirectional Gln efflux.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Scalise M,Pochini L,Pingitore P,Hedfalk K,Indiveri C

doi

10.1016/j.febslet.2015.10.011

subject

Has Abstract

pub_date

2015-11-30 00:00:00

pages

3617-23

issue

23

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(15)00935-7

journal_volume

589

pub_type

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