Abstract:
:The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppressed by the addition of calcitonin. The Z-Phe-Arg-MCA hydrolysing activity increased in the medium through the effect of PTH is considered to be a kind of procathepsin L by Western blotting analysis, and was suppressed by calcitonin addition. Furthermore, monensin inhibited not only the PTH-induced pit formation, but also cysteine proteinase activity in osteoclasts. Therefore, the procathepsin L excreted might be transferred from endothelial reticulum via Golgi and/or via lysosomes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tagami K,Kakegawa H,Kamioka H,Sumitani K,Kawata T,Lenarcic B,Turk V,Katunuma Ndoi
10.1016/0014-5793(94)80522-9subject
Has Abstractpub_date
1994-04-11 00:00:00pages
308-12issue
3eissn
0014-5793issn
1873-3468journal_volume
342pub_type
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