Abstract:
:Escherichia coli evolves H2 via multiple [Ni-Fe]-hydrogenases (Hyd). This activity under hyper- and hypo-osmotic stress was investigated with mutants lacking different Hyd enzymes during glycerol fermentation. Inhibitory effects of hypo-stress on H2 production was stronger at pH 6.5 in wild type and mutants except fhlA, which encodes a transcriptional activator for Hyd-3, compared with the effects of N,N'-dicyclohexylcarbodiimide. These results indicate that Hyd-3 and Hyd-4 are osmosensitive at pH 7.5. Hyd-4 and FhlA are implicated in osmotic stress response at pH 6.5. Hyd-1 and FhlA might be osmosensitive at pH 5.5. Thus, osmosensitivity of Hyd enzymes is a novel property that depends on pH. This is significant for mechanisms of cell osmoregulation and H2 production biotechnology when glycerol is used as a fermentation substrate.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Trchounian K,Trchounian Adoi
10.1016/j.febslet.2013.09.016subject
Has Abstractpub_date
2013-11-01 00:00:00pages
3562-6issue
21eissn
0014-5793issn
1873-3468pii
S0014-5793(13)00701-1journal_volume
587pub_type
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