Escherichia coli multiple [Ni-Fe]-hydrogenases are sensitive to osmotic stress during glycerol fermentation but at different pHs.

Abstract:

:Escherichia coli evolves H2 via multiple [Ni-Fe]-hydrogenases (Hyd). This activity under hyper- and hypo-osmotic stress was investigated with mutants lacking different Hyd enzymes during glycerol fermentation. Inhibitory effects of hypo-stress on H2 production was stronger at pH 6.5 in wild type and mutants except fhlA, which encodes a transcriptional activator for Hyd-3, compared with the effects of N,N'-dicyclohexylcarbodiimide. These results indicate that Hyd-3 and Hyd-4 are osmosensitive at pH 7.5. Hyd-4 and FhlA are implicated in osmotic stress response at pH 6.5. Hyd-1 and FhlA might be osmosensitive at pH 5.5. Thus, osmosensitivity of Hyd enzymes is a novel property that depends on pH. This is significant for mechanisms of cell osmoregulation and H2 production biotechnology when glycerol is used as a fermentation substrate.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Trchounian K,Trchounian A

doi

10.1016/j.febslet.2013.09.016

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

3562-6

issue

21

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(13)00701-1

journal_volume

587

pub_type

杂志文章
  • cDNA encoding the chicken ortholog of the mouse dilute gene product. Sequence comparison reveals a myosin I subfamily with conserved C-terminal domains.

    abstract::We report the cDNA-deduced primary structure of the chicken counterpart of the murine dilute gene product, a member of the myosin I family. Comparison of the chicken and mouse sequences reveals a distinct pattern of domains of high and low sequence conservation. An internal deletion of 25 amino acids probably reflects...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)81123-4

    authors: Sanders G,Lichte B,Meyer HE,Kilimann MW

    更新日期:1992-10-26 00:00:00

  • Iron binding to microsomes and liposomes in relation to lipid peroxidation.

    abstract::The effects of ADP, ATP, citrate and EDTA on iron-dependent microsomal and liposomal lipid peroxidation, and on 59FeCl3 binding to the lipid membranes were measured. The aim was to test if initiation of lipid peroxidation is a site-specific mechanism requiring bound iron. In the absence of chelator, iron was bound to ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(87)80131-x

    authors: Vile GF,Winterbourn CC

    更新日期:1987-05-04 00:00:00

  • Le couplage phosphore-phosphore dans l'adenosine di- et triphosphate.

    abstract::The pH dependence of spin-spin coupling constants J (P-O-P) of ADP and ATP has been determined from the phosphorus NMR spectra at 0 degrees. The variation curves have been interpreted as titration curves. These give different pK values. Comparison with the tripolyphosphate shows the existence of triphosphate chain str...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(70)80243-5

    authors: Ellenberger M,Brehamet L,Villemin M,Toma F

    更新日期:1970-06-01 00:00:00

  • Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1alpha) upregulated E-cadherin expression in HepG2 cells.

    abstract::Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1alpha), a highly inducible transcriptional coactivator regulating energy homeostasis, is down-regulated in hepatoma tissues. To dissect its role in hepato-tumorigenesis, Ingenuity Pathway Analysis was applied to construct pathways affected by P...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2008.01.033

    authors: Lee HJ,Su Y,Lui WY,Chau GY,Yin PH,Lee HC,Chi CW

    更新日期:2008-03-05 00:00:00

  • Map of chartreusin and elsamicin binding sites on DNA.

    abstract::Three DNA restriction fragments designated tyrT, 102-mer and 70-mer, have been used as substrates for footprinting studies using DNase I in the presence of the structurally similar antibiotics chartreusin and elsamicin A. The sequence-selective binding sites of the antibiotics can be mapped in regions which are rich i...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80872-z

    authors: Salas X,Portugal J

    更新日期:1991-11-04 00:00:00

  • Tumor promoter 12-O-tetradecanoylphorbol 13-acetate inhibits mas/angiotensin receptor-stimulated inositol phosphate production and intracellular Ca2+ elevation in the 401L-C3 neuronal cell line.

    abstract::Stimulation of mas-oncogene transfected 401L-C3 cells by angiotensins leads to the production of inositol phosphates. This response shows dose dependence, and has an apparent rank order of potency angiotensin III greater than or equal to angiotensin II much greater than angiotensin I. Preincubation with 12-O-tetradeca...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)81422-x

    authors: Jackson TR,Hanley MR

    更新日期:1989-07-17 00:00:00

  • Denaturation and partial renaturation of a tightly tetramerized DsRed protein under mildly acidic conditions.

    abstract::The red fluorescent protein, DsRed, recently cloned from coral Discosoma sp. has one of the longest fluorescence waves and one of the most complex absorbance spectra among the family of fluorescent proteins. In this work we found that with time DsRed fluorescence decreases under mildly acidic conditions (pH 4.0-4.8) i...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(00)02344-9

    authors: Vrzheshch PV,Akovbian NA,Varfolomeyev SD,Verkhusha VV

    更新日期:2000-12-29 00:00:00

  • Mouse cathepsin K: cDNA cloning and predominant expression of the gene in osteoclasts, and in some hypertrophying chondrocytes during mouse development.

    abstract::We have constructed cDNA clones covering the entire coding region of mouse, human and rabbit preprocathepsin K mRNA for studies on bone turnover. The clone pMCatK-1 for mouse cathepsin K shares 87% nucleotide homology with the corresponding human and rabbit sequences. Analysis of a panel of mouse tissues for tissue di...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(96)00907-6

    authors: Rantakokko J,Aro HT,Savontaus M,Vuorio E

    更新日期:1996-09-16 00:00:00

  • Role of RIP140 in metabolic tissues: connections to disease.

    abstract::The control of physiological processes requires the regulation and coordination of many different signals and is determined in part by the activation and repression of expression of specific target genes. RIP140 is a ligand dependent coregulator of many nuclear receptors that influence such diverse processes as muscle...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2007.11.017

    authors: White R,Morganstein D,Christian M,Seth A,Herzog B,Parker MG

    更新日期:2008-01-09 00:00:00

  • N-terminal heterogeneity of methylamine dehydrogenase from Thiobacillus versutus.

    abstract::The N-terminal processing of MADH from the bacterium T. versutus and the N-terminal heterogeneity of the isolated alpha subunit of the alpha 2 beta 2 protein complex was demonstrated by a combination of Edman sequence analysis of an electroblotted band, in situ digested with pyroglutamate aminopeptidase, and accurate ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80402-g

    authors: Van Beeumen J,Van Driessche G,Huitema F,Duine JA,Canters GW

    更新日期:1993-10-25 00:00:00

  • Contribution of IL-12R mediated feedback loop to Th1 cell differentiation.

    abstract::T helper 1 (Th1) cell fate is induced by overlapping signaling pathways, whose kinetic principles and regulatory motifs are largely unknown. We identified a simple positive feedback loop in the STAT4 signaling pathway, whereby activation by IL-12 leads to the increased expression in IL-12 receptor. A computational ana...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.10.007

    authors: Becskei A,Grusby MJ

    更新日期:2007-11-13 00:00:00

  • An 86 kDa diapause protein 1-like protein is a component of early-staged encapsulation-relating proteins in coleopteran insect, Tenebrio molitor larvae.

    abstract::Recently, we reported two novel early-staged encapsulation-relating proteins (56 kDa and 48 kDa ERPs) isolated from the hemolymph of coleopteran insect, Tenebrio molitor larvae [Cho et al. (1999) Eur. J. Biochem. (in press)]. Here, a cDNA clone for another early-staged encapsulation-relating protein (86 kDa) was isola...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)00608-0

    authors: Cho MY,Choi HW,Moon GY,Kim MH,Kwon TH,Homma K,Natori S,Lee BL

    更新日期:1999-05-28 00:00:00

  • Pertussis toxin reverses the inhibition of insulin secretion caused by [Arg8]vasopressin in rat pancreatic islets.

    abstract::When rat pancreatic islets were incubated with 10(-8) M arginine vasopressin in the presence of 15 mM glucose there was a pronounced inhibition of insulin release in comparison with controls. This inhibitory effect appeared to be specific for vasopressin since it was antagonised by vasopressin antibody. Moreover, pert...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(88)80720-8

    authors: Khalaf LJ,Taylor KW

    更新日期:1988-04-11 00:00:00

  • Binding of a de novo designed peptide to specific glycosaminoglycans.

    abstract::The binding of glycosaminoglycans to a synthetic peptide (SKAQKAQAKQAKQAQKAQKAQAKQAKQW-CONH(2)), consisting of a hybrid consensus heparin binding sequence, is studied using circular dichroism, fluorescence anisotropy and nuclear magnetic resonance techniques. The results unveil certain novel features, most importantly...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(00)01964-5

    authors: Jayaraman G,Wu CW,Liu YJ,Chien KY,Fang JC,Lyu PC

    更新日期:2000-09-29 00:00:00

  • Identification of the axial heme ligands of cytochrome b556 in succinate: ubiquinone oxidoreductase from Escherichia coli.

    abstract::Electron paramagnetic resonance (EPR) and near-infrared magnetic circular dichroism (MCD) have been used to identify the ligands to the cytochrome b556 component of succinate: ubiquinone oxidoreductase (succinate dehydrogenase) from Escherichia coli. The 'highly axial low spin' (HALS) EPR spectrum suggests bis(histidi...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)01189-3

    authors: Peterson J,Vibat C,Gennis RB

    更新日期:1994-11-28 00:00:00

  • Transcriptional and translational control over sodium-glucose-linked transporter (SGLT1) gene expression in adult rat small intestine.

    abstract::We have measured SGLT1 mRNA content and SGLT1-mediated glucose transport at different positions along the small intestine of control and streptozotocin diabetic rats and shown both parameters to be similar but higher in jejunal compared with ileal tissue. No such correlation was seen when comparing measurements of SGL...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(97)00246-9

    authors: Dong R,Srai SK,Debnam E,Smith M

    更新日期:1997-04-07 00:00:00

  • The orientation of the antibiotic peptide maculatin 1.1 in DMPG and DMPC lipid bilayers. Support for a pore-forming mechanism.

    abstract::Maculatin 1.1 is an antimicrobial peptide isolated from the Australian tree frog Litoria genimaculata that adopts an amphipathic, alpha-helical structure in solution. Its orientation and conformation when incorporated to pre-formed DMPG (1,2-dimyristoyl-sn-glycero-3-phosphoglycerol) and DMPC (1,2-dimyristoyl-sn-glycer...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)03313-0

    authors: Chia CS,Torres J,Cooper MA,Arkin IT,Bowie JH

    更新日期:2002-02-13 00:00:00

  • Effect of persistent measles virus infection on protein kinase C activity and c-fos protooncogene expression in neuroblastoma cells.

    abstract::The effect of persistent measles virus infection on c-fos protooncogene and protein kinase C (PKC-I) gene expression in a murine neuroblastoma cell line was studied. Overexpression of c-fos protooncogene by infected NS20Y/MS cells was detected when compared with uninfected NS20Y cells. The level of PKC-I-specific mRNA...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)80757-4

    authors: Wolfson M,Katorza A,Segal S,Rager-Zisman B

    更新日期:1989-07-03 00:00:00

  • Identification of a latent MAP kinase kinase kinase in PC12 cells as B-raf.

    abstract::A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after sti...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)00723-3

    authors: Traverse S,Cohen P

    更新日期:1994-08-15 00:00:00

  • Perspectives on the automatic design of regulatory systems for synthetic biology.

    abstract::Automatic design is based on computational modeling and optimization methods to provide prototype designs to targeted problems in an unsupervised manner. For biological circuits, we need to produce quantitative predictions of cell behavior for a given genotype as consequence of the different molecular interactions. Au...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2012.02.031

    authors: Rodrigo G,Carrera J,Landrain TE,Jaramillo A

    更新日期:2012-07-16 00:00:00

  • Occurrence of heme O in photoheterotrophically growing, semi-anaerobic cyanobacterium Synechocystis sp. PCC6803.

    abstract::Extraction and identification of the non-covalently bound heme groups from crude membrane preparations of photoheterotrophically grown Synechocystis sp. PCC 6803 by reversed phase high performance liquid chromatography and optical spectrophotometry led to the detection of heme O in addition to hemes B and A which latt...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)00821-p

    authors: Peschek GA,Wastyn M,Fromwald S,Mayer B

    更新日期:1995-09-04 00:00:00

  • ATP-dependent peptide release from mitochondria of reticulocytes.

    abstract::ATP-dependent release of TCA-precipitable peptides from mitochondria-containing stroma (MCS) is described. The process is independent of ubiquitin, but is sensitive to hemin and to heat treatment. Neither chloramphenicol nor EGTA inhibit. 50% of the activity is dependent on charged tRNA. The peptides released from MCS...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)80740-9

    authors: Dubiel W,Drung I,Müller M,Rapoport SM

    更新日期:1989-07-03 00:00:00

  • Analysis of PI (phosphatidylinositol)-anchoring antigens in a patient of paroxysmal nocturnal hemoglobinuria (PNH) reveals deficiency of 1F5 antigen (CD59), a new complement-regulatory factor.

    abstract::FACS analysis together with PIPLC treatment was applied to PI-anchoring antigens such as DAF (decay-accerelating factor, CD55), 1F5 antigen (CD59), CD14 and CD16 on the cell surfaces of blood cells from a normal adult and a male patient with paroxysmal nocturnal hemoglubinuria (PNH). Through the extensive analysis, th...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(90)80656-4

    authors: Taguchi R,Funahashi Y,Ikezawa H,Nakashima I

    更新日期:1990-02-12 00:00:00

  • Interaction of the disordered terminal regions of flagellin upon flagellar filament formation.

    abstract::Helical filaments of bacterial flagella are built up by a self-assembly process from thousands of flagellin subunits. To clarify how the disordered terminal regions of flagellin interact upon filament formation, polymerization ability of various terminally truncated fragments was investigated. Fragments deprived of 19...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(02)03859-0

    authors: Gugolya Z,Muskotál A,Sebestyén A,Diószeghy Z,Vonderviszt F

    更新日期:2003-01-30 00:00:00

  • Original involvement of antimicrobial peptides in mussel innate immunity.

    abstract::Recently, the existence and extended diversity of antimicrobial peptides has been revealed in two mussel species. These molecules are classified into four groups according to common features of their primary structure: defensins, mytilins, myticins and mytimycin. In Mytilus galloprovincialis, gene structure reveals sy...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/s0014-5793(00)02192-x

    authors: Mitta G,Vandenbulcke F,Roch P

    更新日期:2000-12-15 00:00:00

  • Phospholipase C and phospholipase A2 are involved in the antiviral activity of human interferon-alpha.

    abstract::Treatment of human amniotic cells (UAC) with human interferon-alpha (Hu-IFN alpha) or phorbol myristate acetate (PMA) resulted in translocation of protein kinase C (PK-C) activity from the cytosol fraction to that of the membranes. Analysis of 32P incorporation into phospholipid fractions and studies of alterations in...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)80635-0

    authors: Premecz G,Markovits A,Bagi G,Farkas T,Földes I

    更新日期:1989-06-05 00:00:00

  • Molecular characterization of a blue visual pigment gene in the fish Astyanax fasciatus.

    abstract::We report here the isolation and sequence determination of a gene closely linked to the Astyanax red visual pigment gene. Reverse transcription polymerase chain reaction assays show that this new gene (B23 Af) and the previously characterized red and green visual pigment genes of Astyanax are all expressed in the eye....

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)81673-n

    authors: Yokoyama R,Yokoyama S

    更新日期:1993-11-08 00:00:00

  • Acidic phospholipids directly inhibit DNA binding of mammalian DNA topoisomerase I.

    abstract::Inhibition of mammalian DNA topoisomerase I by phospholipids was investigated using purified enzyme. Acidic phospholipids inhibited the DNA relaxation activity of topoisomerase I whereas neutral phospholipid, phosphatidylethanolamine, did not. Accumulation of a protein-DNA cleavable complex, an intermediate which is k...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(90)80658-6

    authors: Tamura H,Ikegami Y,Ono K,Sekimizu K,Andoh T

    更新日期:1990-02-12 00:00:00

  • Potato tuber phospholipids contain colneleic acid in the 2-position.

    abstract::Colneleic acid (9-[1'(E),3'(Z)-nonadienyloxy]-8(E)-nonenoic acid) is produced from linoleic acid by the sequential action of 9-lipoxygenase and divinyl ether synthase. We demonstrate that a small fraction of the colneleic acid in potato tubers is esterified in phospholipids. This colneleic acid was released by chemica...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(03)00171-6

    authors: Fauconnier ML,Williams TD,Marlier M,Welti R

    更新日期:2003-03-13 00:00:00

  • Metabolic activation of 1,2-dibromoethane to a free radical intermediate by rat liver microsomes and isolated hepatocytes.

    abstract::A one-electron reductive metabolism of 1,2-dibromoethane (DBE) is described that gives rise to a free radical intermediate, which can be stabilized by a spin trapping agent and detected by electron spin resonance spectroscopy. Using rat liver microsomes or isolated hepatocytes from phenobarbitone pretreated animals, u...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(83)80964-8

    authors: Tomasi A,Albano E,Dianzani MU,Slater TF,Vannini V

    更新日期:1983-08-22 00:00:00