Abstract:
:The tetracycline-controlled transcription system has become one of the most potent systems for experimental manipulations of transcription levels in vivo. Here we report on rtTA variants, which were generated by combining the existing positively regulated Tet repressor domains of rtTA and rtTA-M2 with a modified and multimerized minimal transactivation domain from VP16 (L-domain). A transactivator with multimerized L-domains shows drastically reduced background activity and enhanced transcriptional activation on different tetracycline-responsive promoters. The new rtTA variants require higher doses of doxycycline and display a more linear dose-response curve than the original rtTA or rtTA-M2 proteins.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kämper MR,Gohla G,Schlüter Gdoi
10.1016/s0014-5793(02)02587-5keywords:
subject
Has Abstractpub_date
2002-04-24 00:00:00pages
115-20issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579302025875journal_volume
517pub_type
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