Abstract:
:Hypoxia-inducible factor-1 (HIF-1) is a transcription factor activated by hypoxia. The HIF-1 activation transduction pathway is poorly understood. In this report, we investigated the activation of extracellular regulated kinases (ERK) in hypoxia and their involvement in HIF-1 activation. We demonstrated that in human microvascular endothelial cells-1 (HMEC-1), ERK kinases are activated during hypoxia. Using dominant negative mutants, we showed that ERK1 is needed for hypoxia-induced HIF-1 transactivation activity. Moreover, using a kinase assay and Western blot experiments, we showed that HIF-1alpha is phosphorylated in hypoxia by an ERK-dependent pathway. These results evidence the role of mitogen-activated protein kinase in the transcriptional response to hypoxia.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Minet E,Arnould T,Michel G,Roland I,Mottet D,Raes M,Remacle J,Michiels Cdoi
10.1016/s0014-5793(00)01181-9keywords:
subject
Has Abstractpub_date
2000-02-18 00:00:00pages
53-8issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01181-9journal_volume
468pub_type
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