Abstract:
:Cultured human fibroblasts isolated from embryonic muscle, skin and peripheral nerve tissues were found to accumulate [3H]L-glutamate by a Na(+)-dependent uptake process strongly inhibited by several glutamate/aspartate analogues including D- and L-aspartate, D- and L-threo-3-hydroxyaspartate and L-trans-pyrrolidine-2,4-dicarboxylate but not D-glutamate. It was also reduced by elevated concentrations of K+, Rb+ and Cs+. The values of Km's were 5-20 microM, well within the 'high affinity' region. Variations in the capacity (Vmax) of [3H]L-glutamate uptake did not correlate with the origin (muscle, skin or nerve tissue) of the fibroblasts. The uptake characteristics suggest that it is mediated by a transport system similar to that commonly observed only in brain tissue.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Balcar VJ,Shen J,Bao S,King NJdoi
10.1016/0014-5793(94)80382-xsubject
Has Abstractpub_date
1994-02-14 00:00:00pages
50-4issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(94)80382-Xjournal_volume
339pub_type
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