Abstract:
:Megasphaera elsdenii and Clostridium MP flavodoxins have been investigated by photo-CIDNP techniques. Using time-resolved spectroscopy and external dyes carrying different charges it was possible to assign unambiguously the resonance lines in the NMR-spectra to tyrosine, tryptophan and methionine residues in the two proteins. The results show that Trp-91 in M.elsdenii and Trp-90 in Cl.MP flavodoxin are strongly immobilized and placed directly above the benzene subnucleus of the prosthetic group. The data further indicate that the active sites of the two flavodoxins are extremely similar.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Moonen CT,Hore PJ,Müller F,Kaptein R,Mayhew SGdoi
10.1016/0014-5793(82)81090-9subject
Has Abstractpub_date
1982-11-22 00:00:00pages
141-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(82)81090-9journal_volume
149pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::PTPRO is often silenced by DNA hypermethylation in primary human tumors and cancer cell lines and functions as a tumor suppressor. Here we show that PTPRO is a target of E2F1. In addition, the microRNA cluster miR-17-92, another target of E2F1, participates in PTPRO regulation. PTPRO mRNA was up-regulated during S pha...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.07.017
更新日期:2008-08-20 00:00:00
abstract::Bacteriophage T5 Bg/II/Hind III DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNA1Ser and tRNA2Ser) with anticodons UGA and GGA, respectively. The mai...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80690-5
更新日期:1983-07-11 00:00:00
abstract::Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydroge...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03059-9
更新日期:2001-11-16 00:00:00
abstract::The activity of the Ca-pumping ATPase of cardiac sarcoplasmic reticulum (SR) is controlled by the phosphorylation of the intrinsic regulatory protein phospholamban (PLB), which affects both the apparent Km(Ca) and the Vmax of the transport process. We have investigated the correlation between phosphorylation of PLB an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81201-3
更新日期:1989-02-13 00:00:00
abstract::The tetraspanins associate with various surface molecules and with each other to build a network of molecular interactions, the tetraspanin web. The interaction of tetraspanins with each other seems to be central for the assembly of the tetraspanin web. All tetraspanins studied, CD9, CD37, CD53, CD63, CD81, CD82 and C...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02522-x
更新日期:2002-04-10 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 plays a pivotal role in mediating the nuclear export of KSHV intronless transcripts. We demonstrate that ORF57 localises and dynamically trafficks through the nucleolus. To assess if nucleolar shuttling of ORF57 is important for the export of intronless viral mRNA, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.10.040
更新日期:2009-11-19 00:00:00
abstract::The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01526-x
更新日期:2000-05-12 00:00:00
abstract::The distribution of flux control coefficients of a glycolytic system in starved rat liver has been determined. The flux control coefficient profile in starved conditions is compared with normal fed conditions showing that in the former phosphofructokinase enhances more than 2-fold its flux control coefficient while gl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81360-7
更新日期:1988-06-06 00:00:00
abstract::The in vivo responses of pyruvate dehydrogenase (PDH) complex to starvation and insulin was assessed in heart, diaphragm and red quadriceps muscle. PDH complex activity was decreased by starvation (3.4-10.2-fold), the magnitude of change depending on muscle type. Insulin increased PDH activity in all muscle types. In ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80777-x
更新日期:1989-07-03 00:00:00
abstract::HERC proteins are characterized by having one or more RCC1-like domains as well as a C-terminal HECT domain in their amino acid sequences. This has led researchers to suggest that they may act as both guanine nucleotide exchange factors and E3 ubiquitin ligases. Here we describe a physical interaction between the HECT...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00205-9
更新日期:2003-03-27 00:00:00
abstract::The pH dependence of spin-spin coupling constants J (P-O-P) of ADP and ATP has been determined from the phosphorus NMR spectra at 0 degrees. The variation curves have been interpreted as titration curves. These give different pK values. Comparison with the tripolyphosphate shows the existence of triphosphate chain str...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(70)80243-5
更新日期:1970-06-01 00:00:00
abstract::1,2-sn-Dihexanoylglycerol (HHG) reduced the ATP content of HL-60 cells. This concentration-related (10-100 microM) effect reached a maximum of over 90%, was enantiomerically specific and not accompanied by release of lactate dehydrogenase. Oleoylacetylglycerols (3-100 microM) had no effect on ATP levels while phorbol ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80036-4
更新日期:1987-04-06 00:00:00
abstract::Here we present a method to purify large amounts of highly pure and stably arrested ribosome-nascent chain complexes (RNCs) from Escherichia coli cells. It relies on the combined use of translation-arrest sequences to generate nascent polypeptides of specified length and subsequent tag purification of the RNCs. Moreov...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.06.041
更新日期:2009-07-21 00:00:00
abstract::The functional molecular masses of the vacuolar membrane H+-ATPase in Saccharomyces cerevisiae under two kinetic conditions for ATP hydrolysis were measured by radiation inactivation. When vacuolar membrane vesicles were exposed to gamma-rays from 60Co, the activities catalyzing a single-cycle and multi-cycles of ATP ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80571-x
更新日期:1989-02-27 00:00:00
abstract::The marked difference in auxin sensitivity between plant roots and shoots was studied in terms of auxin-induced stimulation of membrane P-type ATPase. The results suggest the existence in rice seedlings of, at least, two isoforms of soluble proteinous factors (SPF), SPF(I) and SPF(II), which are involved in the stimul...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00527-9
更新日期:1997-06-09 00:00:00
abstract::Amino acid sequencing of a 48/46 kDa glycoprotein from human plantar callus, recognised by antisera raised against the desmosomal cadherins DGII/III, has revealed N-terminal homology to the DNA-derived sequence of human and bovine DGII/III. However, a tryptic fragment has homology only with a bovine clone. We propose ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80929-w
更新日期:1991-07-29 00:00:00
abstract::Blood flukes of the genus Schistosoma currently infect millions of people in tropical and subtropical countries. An enzyme playing a major role in hemoglobin (Hb) degradation by Schistosoma mansoni has been cloned and shown to be highly similar to the human cathepsin D aspartyl proteinase, although presenting a distin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02270-6
更新日期:2002-03-13 00:00:00
abstract::Members of the heat shock protein 70 (Hsp70) family are found in most of the compartments of eukaryotic cells where they play essential roles in protein metabolism. In yeast mitochondria, two Hsp70 proteins are known: Ssc1 and Ssq1. We identified Ecm10 as a third Hsp70 protein in the mitochondrial matrix. Ecm10 shares...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02364-4
更新日期:2000-12-29 00:00:00
abstract::The small GTPase Rho1 plays an essential role in controlling the organization of the actin cytoskeleton and synthesis of the cell wall in the fission yeast Schizosaccharomyces pombe. Here we studied the role of Rho5 whose primary structure is very similar to that of Rho1. It was found that elevated expression of Rho5 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.08.031
更新日期:2005-09-26 00:00:00
abstract::In addition to the selective amplification of cDNA from total RNA by the PCR method, the distinctive properties of ferredoxin-expressing colonies can be used for cloning a ferredoxin cDNA. This strategy for cloning and expressing cDNA in E. coli was applied to a sheep adreno-ferredoxin. The expressed sheep ferredoxin ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81232-b
更新日期:1992-04-20 00:00:00
abstract::The largest mammalian enzyme family is the kinases. Kinases and other nucleotide-binding proteins are key regulators of signal transduction pathways and the mutation or overexpression of these proteins is often the difference between health and disease. As a result, a massive research effort has focused on understandi...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.05.008
更新日期:2013-06-27 00:00:00
abstract::We have shown previously that the skeletal muscle ryanodine receptor mRNA of approximately 16,000 nucleotides codes 5,037 amino acid residues constituting the calcium release channel in skeletal muscle. In this study, RNA blot hybridization analysis shows that the brain contains an RNA species with an estimated size o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81547-d
更新日期:1993-05-10 00:00:00
abstract::The nuclear factor NF-IL6 had been suggested to be responsible for the IL-6-mediated induction of several acute-phase proteins. To obtain evidence for the involvement of NF-IL6 in the induction of acute-phase proteins, we introduced the NF-IL6 gene and its truncated mutant (delNFIL6) gene into a hepatoma cell line Hep...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81103-f
更新日期:1991-10-07 00:00:00
abstract::The rat chromosomal gene for uricase was cloned. The gene spans more than 20 kilobases and the coding region is divided into 8 exons by 7 introns. The organization of the rat uricase gene is so greatly different from that of the soybean gene that the difference may not have been caused only by the removal of some ance...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80789-l
更新日期:1990-05-07 00:00:00
abstract::Protein glycosylation, which is a key post-translational event, is catalysed by the glycosyltransferase family of enzymes. There is an increasing body of evidence to suggest that these enzymes may themselves be glycosylated, possibly as an autocatalytic event. Using a novel in vitro system, we have investigated the ro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02626-1
更新日期:2002-04-24 00:00:00
abstract::Mitochondrial ATP synthase, a major ATP supplier in respiring cells, should be regulated in amount and in activity to respond to the varying demands of cells for ATP. We screened 80 protein kinase inhibitors and found that HeLa cells treated with four inhibitors exhibited reduced mitochondrial ATP synthesis activity. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.10.012
更新日期:2013-11-29 00:00:00
abstract::The effects of mutation of residue tyrosine M210 on the primary donor bacteriochlorophylls have been investigated by near infrared FT-Raman spectroscopy in reaction centres purified from an antenna-deficient strain of Rhodobacter sphaeroides. We find that mutation at the M210 position does not significantly perturb th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80376-5
更新日期:1994-02-14 00:00:00
abstract::Equine platelet beta tropomyosin (247 residues), like rabbit skeletal muscle alpha tropomyosin (284 residues) has a repeating pattern of amino acid residues characteristic of a coiled-coil structure. When compared with the muscle protein, it is extended by 5 residues at the NH2-terminus and possesses two 21 residue de...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80511-0
更新日期:1983-06-13 00:00:00
abstract::Siamycin I disrupts growth and quorum sensing in Enterococcus faecalis. Using purified intact protein, we demonstrate here that quorum membrane sensor kinase FsrC is a direct target of siamycin I, reducing pheromone-stimulated autophosphorylation activity by up to 91%. Inhibition was non-competitive with ATP as substr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.07.026
更新日期:2011-09-02 00:00:00
abstract::Lipopolysaccharides (LPS, endotoxin) stimulate mononuclear cells to release cytokines which initiate endotoxic effects. Interaction of LPS at low concentrations with target cells is CD14-dependent whereas at high LPS concentrations it is CD14-independent. Here, we demonstrate by resonance energy transfer (RET) techniq...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01338-5
更新日期:1996-12-16 00:00:00