N-linked glycosylation of platelet P2Y12 ADP receptor is essential for signal transduction but not for ligand binding or cell surface expression.

Abstract:

:P(2)Y(12) receptor is a G(i)-coupled adenosine diphosphate (ADP) receptor with a critical role in platelet aggregation. It contains two potential N-linked glycosylation sites at its extra cellular amino-terminus, which may modulate its activity. Studies of both tunicamycin treatment and site-directed mutagenesis have revealed a dispensable role of the N-linked glycosylation in the receptor's surface expression and ligand binding activity. However, the non-glycosylated P(2)Y(12) receptor is defective in the P(2)Y(12)-mediated inhibition of the adenylyl cyclase activity. Thus the study uncovers an unexpected vital role of N-linked glycans in receptor's signal transducing step but not in surface expression or ligand binding.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Zhong X,Kriz R,Seehra J,Kumar R

doi

10.1016/S0014-5793(04)00191-7

keywords:

subject

Has Abstract

pub_date

2004-03-26 00:00:00

pages

111-7

issue

1-3

eissn

0014-5793

issn

1873-3468

pii

S0014579304001917

journal_volume

562

pub_type

杂志文章
  • Structural basis for recognition of ubiquitinated cargo by Tom1-GAT domain.

    abstract::Tom1 (Target of Myb1) is suggested to be involved in the transport of ubiquitinated proteins, through the interaction of its GAT (GGA and Tom1) domain with ubiquitin. Here, we demonstrate that the three-helix bundle of Tom1-GAT has two ubiquitin-binding sites recognizing the hydrophobic Ile44 surface of ubiquitin. The...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2005.08.076

    authors: Akutsu M,Kawasaki M,Katoh Y,Shiba T,Yamaguchi Y,Kato R,Kato K,Nakayama K,Wakatsuki S

    更新日期:2005-10-10 00:00:00

  • Biochemical characterization of the p51 sub-unit of human immunodeficiency virus reverse transcriptase in homo- and heterodimeric recombinant forms of the enzyme.

    abstract::The biochemical properties of the p51 subunit of HIV-1 reverse transcriptase (RT) were studied in order to understand its role in the heterodimeric form p66/p51 found in virions. A recombinant form of RT, p51/p51, expressed in yeast, was purified and characterized. The enzyme was affinity labeled using a 5' modified o...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80202-r

    authors: el Dirani-Diab R,Andreola ML,Nevinsky G,Tharaud D,Barr PJ,Litvak S,Tarrago-Litvak L

    更新日期:1992-04-13 00:00:00

  • Crystallization and preliminary X-ray analysis of methylamine-treated alpha 2-macroglobulin and 3 alpha 2-macroglobulin-proteinase complexes.

    abstract::Crystals of methylamine-treated alpha 2-macroglobulin (alpha 2M-MA), alpha 2-macroglobulin in complex with two molecules of trypsin, alpha 2M-T2, one molecule of plasmin, alpha 2M-PL, and one molecule of plasmin followed by methylamine-treatment, alpha 2M-PL(MA), have reproducibly been obtained using ammonium sulfate ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80881-3

    authors: Andersen GR,Jacobsen L,Thirup S,Nyborg J,Sottrup-Jensen L

    更新日期:1991-11-04 00:00:00

  • Nucleoside analogues previously found to be inactive against HIV may be activated by simple chemical phosphorylation.

    abstract::Nucleoside analogues previously found to be inactive against the human immunodeficiency virus (HIV) may be activated by simple chemical derivatisation. As part of our effort to deliver masked phosphates inside living cells we have discovered that certain phosphate triester derivatives of inactive nucleoside analogues ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)81580-s

    authors: McGuigan C,Kinchington D,Wang MF,Nicholls SR,Nickson C,Galpin S,Jeffries DJ,O'Connor TJ

    更新日期:1993-05-17 00:00:00

  • Casein-derived bioactive phosphopeptides: role of phosphorylation and primary structure in promoting calcium uptake by HT-29 tumor cells.

    abstract::Casein phosphopeptides beta-CN(1-25)4P and alpha(s1)-CN(59-79)5P, from beta- and alpha(s1)-casein, respectively, both carrying the characteristic 'acidic motif' Ser(P)-Ser(P)-Ser(P)-Glu-Glu, were chemically synthesized and administered to HT-29 cells differentiated in culture, which are a used model of intestinal epit...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(03)00741-5

    authors: Ferraretto A,Gravaghi C,Fiorilli A,Tettamanti G

    更新日期:2003-09-11 00:00:00

  • Multiplicite de la phosphatase alcaline cerebrale de differentes especes.

    abstract::The alkaline phosphatase of beef, horse, pig and sheep brain have been purified. In each case, the alkaline phosphatase activity can be separated into two or three components by DEAE-cellulose chromatography and by electrophoresis. Although the properties of these enzyme fractions are very similar, there are sufficien...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(70)80073-4

    authors: Brunel C,Cathala G

    更新日期:1970-02-16 00:00:00

  • Luminescence of peptide-bound terbium ions. Determination of binding constants.

    abstract::Luminescence of Tb3+ ions bound to a calmodulin fragment has been studied. It is shown that during their lifetime excited ions dissociate from the peptide. If concentration of free peptide is high enough they can be coordinated again. As a consequence, observed terbium luminescence lifetime and intensity depends not o...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80464-e

    authors: Dadlez M,Góral J,Bierzyński A

    更新日期:1991-04-22 00:00:00

  • Brefeldin A and the endocytic pathway. Possible implications for membrane traffic and sorting.

    abstract::A number of recent observations have suggested that the endocytic and biosynthetic pathways may share fundamentally similar transport mechanisms at the molecular level. Some of the more striking of these suggestions have come from a comparison of the effects of the macrocyclic lactone brefeldin A (BFA) on endosomes an...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/0014-5793(92)80908-y

    authors: Hunziker W,Whitney JA,Mellman I

    更新日期:1992-07-27 00:00:00

  • Cellular strategies for proteolytic targeting during migration and invasion.

    abstract::Cell migration over or through the extracellular matrix (ECM) is an integral feature of both physiological and pathological processes. Regulation of the changing cell-ECM interactions involved can be effected by proteolysis and requires strict spatial and temporal targeting of proteinase activity. The versatile use of...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/s0014-5793(01)02845-9

    authors: Ellis V,Murphy G

    更新日期:2001-09-28 00:00:00

  • High protein mobility in skinned rabbit muscle fibres observed by 1H NMR spectroscopy.

    abstract::1H NMR spectra of skinned rabbit muscle fibers show a group of relatively sharp resonance lines which presumably originate from highly mobile protein domains. Comparison with the spectrum of myosin subfragment 1 suggests that these signals may come at least partly from mobile regions of the myosin head. NMR could poss...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80063-m

    authors: Kalbitzer HR,Schrumpf M,Wray J

    更新日期:1992-02-24 00:00:00

  • Kinetic properties of cobalt--iron hybrid hemoglobins.

    abstract::The replacement of O2 with CO was studied on cobalt-iron hemoglobin hybrids. Both proto- and mesocobalt hemes were used for the reconstitution. In the oxy quaternary conformation no difference is observed between alpha- and beta-subunits when only proto hemes are present in the hybrid (k4 = 30 s-1, k'4/l'4 = 2.5). If ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(83)80150-1

    authors: Oertle M,Winterhalter KH,Di Iorio EE

    更新日期:1983-03-07 00:00:00

  • A photo-CIDNP study of the active sites of Megasphaera elsdenii and Clostridium MP flavodoxins.

    abstract::Megasphaera elsdenii and Clostridium MP flavodoxins have been investigated by photo-CIDNP techniques. Using time-resolved spectroscopy and external dyes carrying different charges it was possible to assign unambiguously the resonance lines in the NMR-spectra to tyrosine, tryptophan and methionine residues in the two p...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(82)81090-9

    authors: Moonen CT,Hore PJ,Müller F,Kaptein R,Mayhew SG

    更新日期:1982-11-22 00:00:00

  • Astrocyte differentiation mediated by LIF in cooperation with BMP2.

    abstract::Leukemia inhibitory factor (LIF) and bone morphogenetic protein (BMP) 2 signal via different receptor systems. We have recently demonstrated that simultaneous stimulation of fetal mouse neuroepithelial cells with these distinct types of cytokines synergistically induces astrocyte differentiation in a 2-day culture. He...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)00997-7

    authors: Nakashima K,Yanagisawa M,Arakawa H,Taga T

    更新日期:1999-08-20 00:00:00

  • A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes.

    abstract::Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1002/1873-3468.12620

    authors: Nishi-Tatsumi M,Yahagi N,Takeuchi Y,Toya N,Takarada A,Murayama Y,Aita Y,Sawada Y,Piao X,Oya Y,Shikama A,Masuda Y,Kubota M,Izumida Y,Matsuzaka T,Nakagawa Y,Sekiya M,Iizuka Y,Kawakami Y,Kadowaki T,Yamada N,Shimano

    更新日期:2017-04-01 00:00:00

  • Cephalopod alcohol dehydrogenase: purification and enzymatic characterization.

    abstract::Octopus, squid and cuttle-fish organs were examined for alcohol dehydrogenase activity. Only one form was detectable, with properties typical of mammalian class III alcohol dehydrogenase. The corresponding protein was purified from octopus and enzymatically characterized. Ion-exchange and affinity chromatography produ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80934-m

    authors: Rosario Fernández M,Jörnvall H,Moreno A,Kaiser R,Parés X

    更新日期:1993-08-16 00:00:00

  • Solubilization of angiotensin II receptors in rat glomeruli.

    abstract::125I-labelled angiotensin II (A II) specifically binds to solubilized receptors extracted from rat isolated glomeruli using CHAPS (3-[3-( cholamidopropyl ) dimethylammonio ]-1-propanesulfonate). The yield of solubilization of the binding sites was 3.3%. Equilibrium was reached after 15-20 min and specific binding repr...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(84)81321-6

    authors: Chansel D,Ardaillou N,Ardaillou R

    更新日期:1984-05-21 00:00:00

  • Na(+)-driven ATP synthesis in Methanobacterium thermoautotrophicum and its differentiation from H(+)-driven ATP synthesis by rhodamine 6G.

    abstract::Rhodamine 6G (3 microM) effectively inhibited delta pH-driven ATP synthesis in Methanobacterium thermoautotrophicum while delta pNa-driven ATP synthesis was not affected by it. Rhodamine 6G inhibited Mg(2+)-stimulated ATPase activity of membrane vesicles prepared from these cells but the ATPase catalytic sector detach...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)00535-4

    authors: Smigán P,Majerník A,Greksák M

    更新日期:1994-06-27 00:00:00

  • Thrombopoietin, c-Mpl ligand, induces tyrosine phosphorylation of Tyk2, JAK2, and STAT3, and enhances agonists-induced aggregation in platelets in vitro.

    abstract::We investigated in vitro effects of recombinant human thrombopoietin (TPO), or c-Mpl ligand, on human platelets. TPO induced rapid dose-dependent tyrosine phosphorylation of several proteins. We identified Janus tyrosine kinases, Tyk2 and JAK2, and a member of STAT (signal transducers and activators of transcription) ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)01072-m

    authors: Ezumi Y,Takayama H,Okuma M

    更新日期:1995-10-23 00:00:00

  • Leukotriene A4 hydrolase, a bifunctional enzyme. Distinction of leukotriene A4 hydrolase and aminopeptidase activities by site-directed mutagenesis at Glu-297.

    abstract::We previously obtained evidence for intrinsic aminopeptidase activity for leukotriene (LT)A4 hydrolase, an enzyme characterized to specifically catalyse the hydrolysis of LTA4 to LTB4, a chemotactic compound. From a sequence homology search between LTA4 hydrolase and several aminopeptidases, it became clear that they ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80806-r

    authors: Minami M,Bito H,Ohishi N,Tsuge H,Miyano M,Mori M,Wada H,Mutoh H,Shimada S,Izumi T

    更新日期:1992-09-14 00:00:00

  • Ratio-fluorescence microscopy of lipid oxidation in living cells using C11-BODIPY(581/591).

    abstract::A ratio-fluorescence assay was developed for on-line localization and quantification of lipid oxidation in living cells. The assay explores the oxidative sensitivity of C11-BODIPY(581/591). Upon oxidation, the fluorescence of this fluorophore shifts from red to green. The probe incorporates readily into cellular membr...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)00696-1

    authors: Pap EH,Drummen GP,Winter VJ,Kooij TW,Rijken P,Wirtz KW,Op den Kamp JA,Hage WJ,Post JA

    更新日期:1999-06-25 00:00:00

  • Gene relaxation and aging: changes in the abundance of rat ventral prostate SGP-2 (clusterin) and ornithine decarboxylase mRNAs.

    abstract::Sulfated glycoprotein 2 (SGP-2) mRNA progressively increased in the ventral prostate of the aging rat, reaching, at 24 months, 4-fold higher than at 3 months. Ornithine decarboxylase (ODC) mRNA peaked at 6 months (4-fold increase), and at 12 and 24 months was maintained at higher levels than at 3 months. ODC enzymatic...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)00609-1

    authors: Bettuzzi S,Strocchi P,Marinelli M,Astancolle S,Davalli P,Corti A

    更新日期:1994-07-18 00:00:00

  • The spectrin repeat: a structural platform for cytoskeletal protein assemblies.

    abstract::Spectrin repeats are three-helix bundle structures which occur in a large number of diverse proteins, either as single copies or in tandem arrangements of multiple repeats. They can serve structural purposes, by coordination of cytoskeletal interactions with high spatial precision, as well as a 'switchboard' for inter...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/s0014-5793(01)03304-x

    authors: Djinovic-Carugo K,Gautel M,Ylänne J,Young P

    更新日期:2002-02-20 00:00:00

  • Determination and cellular localization of adenylyl cyclase isozymes expressed in embryonic chick heart.

    abstract::Mammalian heart has been reported to express AC isozymes (types V and VI) that are inhibited by < microM [Ca2+]; avian heart has been reported to express adenylyl cyclase activity that is inhibited by < microM [Ca2]. We have used reverse transcription polymerase chain reaction (RT-PCR) to determine that type V and VI ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)01084-r

    authors: Yu HJ,Unnerstall JR,Green RD

    更新日期:1995-10-23 00:00:00

  • The mitochondrial ABC transporter Atm1p functions as a homodimer.

    abstract::The ATP-binding cassette (ABC) transporters constitute one of the largest families of proteins in evolution. The ATM1 gene of the yeast Saccharomyces cerevisiae encodes an ABC protein, which is localized to the mitochondrial inner membrane. A deletion of ATM1 results in the accumulation of up to a 30-fold excess of mi...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2004.05.051

    authors: Chloupková M,Reaves SK,LeBard LM,Koeller DM

    更新日期:2004-07-02 00:00:00

  • A conjugate of the A1 peptide of cholera toxin and the lectin of Wisteria floribunda that activates the adenylate cyclase of intact cells.

    abstract::The active A1 peptide of cholera toxin was linked by a disulphide bond to the lectin of Wisteria floribunda. The resulting conjugate activated the adenylate cyclase of intact U937 or K562 cells at the same concentrations as native toxin did, but to a greater extent. Activation was inhibited by N-acetyl-D-galactosamine...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(83)80035-0

    authors: van Heyningen S

    更新日期:1983-11-28 00:00:00

  • Reducing jasmonic acid levels causes ein2 mutants to become ethylene responsive.

    abstract::It has previously been shown that jasmonic acid affects the ethylene signaling pathway. EIN2 is a central component of ethylene signaling that is downstream of the receptors. EIN2 has previously been shown to be required for ethylene responses. We found that reducing jasmonic acid levels, either mutationally or chemic...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2012.11.030

    authors: Kim J,Patterson SE,Binder BM

    更新日期:2013-01-16 00:00:00

  • Mechanically induced electrical responses in murine mammary epithelial cells in primary culture.

    abstract::In mouse mammary epithelial cells in primary culture, mechanical stimulation of a cell induced in other cells within the same colony a short depolarization of less than 15 mV with a duration of 1-8 s and a subsequent, prominent hyperpolarization of 6 mV lasting 10-40 s. Epidermal growth factor induces a spontaneous hy...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(87)80514-8

    authors: Enomoto K,Furuya K,Maeno T,Edwards C,Oka T

    更新日期:1987-10-19 00:00:00

  • The constituents of storage granules in the dermal glands of Xenopus laevis. Structure of a basic polypeptide deduced from cloned cDNA.

    abstract::Mature dermal glands of Xenopus laevis contain storage granules with a characteristic ellipsoid shape. A few major proteins are present in these granules, including two with the same amino-terminal sequence and apparent molecular masses of 26 and 28 kDa. Using antibodies against these proteins, positive clones were is...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)80644-1

    authors: Berger H,Kreil G

    更新日期:1989-06-05 00:00:00

  • Insulin-like growth factor I supports differentiation of cultured osteoblast-like cells.

    abstract::Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I w...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(84)81015-7

    authors: Schmid C,Steiner T,Froesch ER

    更新日期:1984-07-23 00:00:00

  • c-Jun/AP-1 transcription factor regulates laminin-1-induced neurite outgrowth in human bone marrow mesenchymal stem cells: role of multiple signaling pathways.

    abstract::In this study, we demonstrated that laminin-1 (LM-1) induces neurite outgrowth and enhances the expression of neurofilament-L and MAP2 in human bone marrow mesenchymal stem cells (MSCs). The c-Jun transcription factor was strongly activated by LM-1 during neurite induction. Suppression of c-Jun inhibited the expressio...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2011.04.072

    authors: Mruthyunjaya S,Rumma M,Ravibhushan G,Anjali S,Padma S

    更新日期:2011-06-23 00:00:00