Abstract:
:Dried cells of Brevibacterium ammoniagenes are a good enzyme source for the preparative synthesis of CoA from pantothenic acid, L-cysteine and ATP. A problem with this synthesis is that the CoA synthesis is repressed by negative feedback inhibition by CoA to pantothenate kinase, the first step enzyme for the biosynthesis of CoA, which catalyses phosphorylation of pantothenic acid or pantetheine. As the inhibition operates only at this step, a further increased yield is possible if the enzymic phosphorylation step is replaced with chemical synthesis. Yields from phosphorylated substrates are more than 10-times higher than those from pantothenic acid or pantetheine (33 g/l from phosphopantothenic acid with a molar yield of 86%; 115 g/l from phosphopantetheine with a molar yield of 100%).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Shimizu S,Komaki R,Tani Y,Yamada Hdoi
10.1016/0014-5793(83)80091-xsubject
Has Abstractpub_date
1983-01-24 00:00:00pages
303-6issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(83)80091-Xjournal_volume
151pub_type
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