Analogs of human epidermal growth factor which partially inhibit the growth factor-dependent protein-tyrosine kinase activity of the epidermal growth factor receptor.

abstract：:We have analyzed the specificity of the actinomycin D-DNA interaction. The 'footprint' method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X no...

journal_title：FEBS letters

authors： Scamrov AV,Beabealashvilli RS

更新日期：1983-11-28 00:00:00

abstract：:The cyclic AMP phosphodiesterases type 4 (PDE4s) are expressed in a cell specific manner, with intracellular targeting directed by unique N-terminal anchor domains. All long form PDE4s are phosphorylated and activated by PKA phosphorylation within their upstream conserved region 1 (UCR1). Here, we identify and charact...

journal_title：FEBS letters

authors： Byrne AM,Elliott C,Hoffmann R,Baillie GS

更新日期：2015-03-12 00:00:00

abstract：:We propose a dynamic model for the competition between exon-exon ligation and 3'-end hydrolysis valid for sequentially folded pre-mRNA introns of group I. This model accounts for the delay in the formation of conserved helix P10 until the 5' exon has been cleaved, a requirement to prevent hydrolysis at the 3' end of t...

journal_title：FEBS letters

authors： Fernández A

更新日期：1992-02-03 00:00:00

abstract：:Cold-stable and cold-labile microtubules were prepared by two cycles of assembly and disassembly and two periods of exposure to cold. The cold-labile preparations were shown to contain a higher proportion of a high molecular mass microtubule-associated protein (MAP 2) than cold-stable preparations. The cold-stable pre...

journal_title：FEBS letters

authors： Hesketh JE

更新日期：1984-04-24 00:00:00

abstract：:Human milk cholesterol esterase (bile salt-activated lipase) plays a role in the dietary uptake of triacylglyceride and cholesteryl ester. The activities toward these substrates are mediated through a unique bile salt-activated mechanism. Previously, it has been proposed that a necessary step in this process is prior ...

journal_title：FEBS letters

authors： Loomes KM,Senior HE

更新日期：1997-04-01 00:00:00

abstract：:A novel protein that binds specifically to the intracellular domain of the p55 tumor necrosis factor (TNF) receptor was cloned by two-hybrid screening of a HeLa cell cDNA library. Data bank searches revealed high sequence similarity of the protein (55.11) to yeast, nematode and plant proteins, whose functions are yet ...

journal_title：FEBS letters

authors： Boldin MP,Mett IL,Wallach D

更新日期：1995-06-19 00:00:00

abstract：:ROPs constitute a family of plant-specific, RHO-like small GTPases that serve as molecular switches in a wide range of signaling pathways. The activities of ROPs are regulated by guanine nucleotide exchange factors (GEFs). ROP11, a member of the ROP GTPase family in Arabidopsis, is a negative regulator of multiple ABA...

journal_title：FEBS letters

authors： Li Z,Liu D

更新日期：2012-05-07 00:00:00

abstract：:We have investigated the importance of the degree of sialylation when an acute phase glycoprotein, alpha 1-proteinase inhibitor (alpha 1-Pi), was analysed both by affinity chromatography on concanavalin A (Con A)-Sepharose and by crossed immuno-affinoelectrophoresis (CIAE) using Con A in the first dimension. Human alp...

journal_title：FEBS letters

authors： Mallet B,Franc JL,Zattara MC

更新日期：1990-03-12 00:00:00

abstract：:The relationship between different amino acids at the beta 6 position of hemoglobin and tetramer stability was addressed by a site-directed mutagenesis approach. Precipitation rates during mechanical agitation of oxyhemoglobins with Gln, Ala, Val, Leu and Trp at the beta 6 position increased 2, 5, 13, 21 and 53 times,...

journal_title：FEBS letters

authors： Adachi K,Kim JY,Konitzer P,Asakura T,Saviola B,Surrey S

更新日期：1993-01-02 00:00:00

abstract：:Various properties have been evaluated for the binding to tissue culture substrata of proteolytic fragments of human plasma or cellular fibronectins containing complementary sequences from the individual and alternatively spliced chains, since related fragments are known to yield differing adhesive responses from cell...

journal_title：FEBS letters

authors： Lewandowska K,Kaetzel CS,Zardi L,Culp LA

更新日期：1988-09-12 00:00:00

abstract：:In cultured rat pituitary gonadotrophs, gonadotropin-releasing hormone (GnRH) induces rapid hyperpolarization of the cell membrane and causes cessation of the spontaneous electrical activity present in non-stimulated cells. This initial response to GnRH is followed by slow oscillations of membrane potential (Vm) which...

journal_title：FEBS letters

authors： Kukuljan M,Stojilković SS,Rojas E,Catt KJ

更新日期：1992-04-13 00:00:00

abstract：:TATA-box binding factor TFIID is one of the key factors in transcriptional activation. Surprisingly, the yeast TFDII protein [(1989) Nature 341, 299-303; (1989) Cell 56, 1173-1181; (1989) Proc. Natl. Acad. Sci USA 86, 7785-7789] reveals only limited homology with other DNA-binding proteins. From computer-assisted sear...

journal_title：FEBS letters

authors： Stucka R,Feldmann H

更新日期：1990-02-26 00:00:00

abstract：:We have expressed in E. coli segments of the rod portion of rabbit skeletal fast muscle myosin and compared physical properties of two different species, LMM-30 and LMM-30C'. LMM-30 consists of 263 amino acids including the original C-terminus of myosin heavy chain. LMM-30C' is colinear with LMM-30, but is devoid of 1...

journal_title：FEBS letters

authors： Maeda K,Rösch A,Maéda Y,Kalbitzer HR,Wittinghofer A

更新日期：1991-04-09 00:00:00

abstract：:VinJ is an amidohydrolase belonging to the serine peptidase family that catalyzes the hydrolysis of the terminal aminoacyl moiety of a polyketide intermediate during the biosynthesis of vicenistatin. Herein, we report the crystal structure of VinJ. VinJ possesses a unique hydrophobic tunnel for the recognition of the ...

journal_title：FEBS letters

authors： Shinohara Y,Miyanaga A,Kudo F,Eguchi T

更新日期：2014-03-18 00:00:00

abstract：:Stimulated neutrophils exhibit coordinated sinusoidal oscillations in filamentous actin content and cellular shape. We investigated the effect of inhibition of the small G protein Rho on neutrophil actin polymerization, shape changes and oscillations using a genetically engineered toxin that enters cells and selective...

journal_title：FEBS letters

authors： Ehrengruber MU,Boquet P,Coates TD,Deranleau DA

更新日期：1995-09-25 00:00:00

abstract：:Small RNAs are non-coding regulatory RNA molecules that control gene expression by mediating mRNA degradation, translational inhibition, or chromatin modification. Virus-derived small RNAs induce silencing of viral RNAs and are essential for antiviral defense in both animal and plant systems. The role of host endogeno...

journal_title：FEBS letters

authors： Jin H

更新日期：2008-08-06 00:00:00

abstract：:Changes in troponin C (TnC) conformation upon Ca(2+) binding forms the basis for regulatory and structural functions of TnC molecules. In the present study, Ca(2+)-induced conformational changes in TnC were observed by mechanical measurements. TnC films were prepared by drying or electrospraying TnC solutions, cross-l...

journal_title：FEBS letters

authors： Bukatina AE,Morozov VN,Gusev NB,Sieck GC

更新日期：2002-07-31 00:00:00

abstract：:Acetylcholine (1-10 microM) depolarized the membrane and stimulated glucose-induced bursts of electrical activity in mouse pancreatic B-cells. The acetylcholine effects were mimicked by muscarine while nicotine had no effect on membrane potential. Pirenzepine, an antagonist of the classical M1-type muscarinic receptor...

journal_title：FEBS letters

authors： Santos RM,Rojas E

更新日期：1989-06-05 00:00:00

abstract：:The YukD protein in Bacillus subtilis was identified in a hidden Markov model (HMM) search as being related in sequence to ubiquitin. By solving the crystal structure we show that YukD adopts a fold that is most closely related to ubiquitin, yet has the shortest C-terminal tail of all known ubiquitin-like proteins. Th...

journal_title：FEBS letters

authors： van den Ent F,Löwe J

更新日期：2005-07-04 00:00:00

abstract：:In yeast, three AAA superfamily metalloproteases (Yme1p, Afg3p and Rca1p) are localized to the mitochondrial inner membrane where they perform roles in the assembly and turnover of the respiratory chain complexes. We have investigated the function of the proposed human orthologue of yeast Yme1p, encoded by the YME1L g...

journal_title：FEBS letters

authors： Shah ZH,Hakkaart GA,Arku B,de Jong L,van der Spek H,Grivell LA,Jacobs HT

更新日期：2000-08-04 00:00:00

abstract：:The cDNAs of several snake venom zinc endopeptidases code for a putative propeptide, which includes the conserved cysteine-containing sequence PKMCGVT. It has been suggested that binding of the cysteine thiol function to the active-site zinc, resulting in inactivation of the catalytic domain, occurs in a mode similar ...

journal_title：FEBS letters

authors： Grams F,Huber R,Kress LF,Moroder L,Bode W

更新日期：1993-11-29 00:00:00

abstract：:In human colon carcinoma HT-29 Glc(-/+) cells, L-arginine is the common precursor of polyamines which are absolutely necessary for cellular proliferation and nitric oxide (NO) with reported anti-proliferative activity. The aim of the present work was to test the effect of the NO donor sodium nitroprusside (SNP) on pol...

journal_title：FEBS letters

authors： Blachier F,Robert V,Selamnia M,Mayeur C,Duee PH

更新日期：1996-11-04 00:00:00

abstract：:The key mechanism in prion disease is the conversion of cellular prion protein into an altered, pathogenic conformation, in which cellular mechanisms play a poorly understood role. Both forms of prion protein are lipid-anchored and reside in rafts that appear to protect the native conformation against conversion. Neur...

journal_title：FEBS letters

authors： Morris RJ,Parkyn CJ,Jen A

更新日期：2006-10-09 00:00:00

abstract：:Matrix metalloproteinase-13 (MMP-13) plays a critical role in parathyroid hormone (PTH)-induced bone resorption. PTH acts via protein kinase A (PKA) to phosphorylate and stimulate the transactivation of Runx2 for MMP-13 promoter activation. We show here that PTH stimulated Runx2 phosphorylation in rat osteoblastic cel...

journal_title：FEBS letters

authors： Selvamurugan N,Shimizu E,Lee M,Liu T,Li H,Partridge NC

更新日期：2009-04-02 00:00:00

abstract：:Heparin was found to stimulate the phosphorylation of histone H1 but not protamine sulfate catalyzed by Ca2+/phospholipid-dependent protein kinase (protein kinase C or PKC). The effect of heparin on histone H1 phosphorylation appeared to be due to an increase in phosphatidylserine affinity for PKC activation in the pr...

journal_title：FEBS letters

authors： Goueli SA,Hanten JA,Ahmed K

更新日期：1991-05-06 00:00:00

abstract：:The role of two positively charged amino acid residues located at the active site of Escherichia coli maltodextrin phosphorylase was investigated by site-directed mutagenesis. Substitution of Lys539 by an arginine caused a 600-fold reduction, substitution of Arg534 by a glutamine caused an even larger 7000-fold reduct...

journal_title：FEBS letters

authors： Schinzel R,Drueckes P

更新日期：1991-07-29 00:00:00

abstract：:Using PCR-based techniques, we have isolated and sequenced the full-length cDNAs that encode the human neuronal nAChR subunits alpha3,4,5,7 and beta2,3,4 in the neuroblastoma cell lines SH-SY5Y and/or IMR-32. The predicted nAChR beta3- and beta4-subunit proteins contain 458 and 498 amino acids, respectively. Except fo...

journal_title：FEBS letters

authors： Groot Kormelink PJ,Luyten WH

更新日期：1997-01-06 00:00:00

abstract：:The relevance of MDR-1 gene expression to the multidrug resistance phenotype was investigated. Drug-resistant cells, KB-V1 and MCF7/ADR, constantly expressed mRNA of the MDR-1 gene and were more resistant to vinblastine and adriamycin than drug-sensitive cells, KB-3-1 and MCF7. The drug efflux rate of KB-V1 was the sa...

journal_title：FEBS letters

authors： Kim HM,Oh GT,Hong DH,Kim MS,Kang JS,Park SM,Han SB

更新日期：1997-07-21 00:00:00

abstract：:The replacement of O2 with CO was studied on cobalt-iron hemoglobin hybrids. Both proto- and mesocobalt hemes were used for the reconstitution. In the oxy quaternary conformation no difference is observed between alpha- and beta-subunits when only proto hemes are present in the hybrid (k4 = 30 s-1, k'4/l'4 = 2.5). If ...

journal_title：FEBS letters

authors： Oertle M,Winterhalter KH,Di Iorio EE

更新日期：1983-03-07 00:00:00

abstract：:LncRNAs have a critical role in the regulation of cellular processes such as cancer progression and metastasis. In the present study, we confirmed that TUG1 was overexpressed in bladder cancer tissues and established cell lines. Knockdown of TUG1 inhibited bladder cancer cell metastasis both in vitro and in vivo. Furt...

journal_title：FEBS letters

authors： Tan J,Qiu K,Li M,Liang Y

更新日期：2015-10-07 00:00:00