Abstract:
:Plasma membrane, sarco-endoplasmic reticulum and secretory pathway Ca2+-ATPases (designated PMCA, SERCA and SPCA) regulate intracellular Ca2+ in animal cells. The presence of PMCA, and the absence of SERCA, in sea urchin sperm is known. By using inhibitors of Ca2+-ATPases, we now show the presence of SPCA and Ca2+ store in sea urchin sperm, which refills by SPCA-type pumps. Immunofluorescence shows SPCA localizes to the mitochondrion. Ca2+ measurements reveal that approximately 75% of Ca2+ extrusion is by Ca2+ ATPases and 25% by Na+ dependent Ca2+ exchanger/s. Bisphenol, a Ca2+ ATPase inhibitor, completely blocks the acrosome reaction, indicating the importance of Ca2+-ATPases in fertilization.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Gunaratne HJ,Vacquier VDdoi
10.1016/j.febslet.2006.06.019subject
Has Abstractpub_date
2006-07-10 00:00:00pages
3900-4issue
16eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00722-8journal_volume
580pub_type
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