Abstract:
:Protein D2 forms the water-filled pore across the outer membrane of Pseudomonas aeruginosa and allows the penetration of imipenem. We cloned the protein D2 gene by the antibody screening technique. When the imipenem-resistant mutant lacking protein D2 harbored the plasmid with the cloned D2 gene, the mutant overproduced protein D2 in the outer membrane. These transformants exhibited fully-restored imipenem susceptibility. The results prove unequivocally that protein D2 forms the imipenem-permeable pore in the P. aeruginosa outer membrane.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yoneyama H,Nakae Tdoi
10.1016/0014-5793(91)80582-nsubject
Has Abstractpub_date
1991-06-03 00:00:00pages
177-9issue
2eissn
0014-5793issn
1873-3468journal_volume
283pub_type
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