Abstract:
:Pyrroloquinoline quinone (PQQ) has been claimed to be a component of glutamate decarboxylase from Escherichia coli on the basis of a frequently used procedure in which the protein is extracted with hexanol. We demonstrate that if pyridoxal phosphate (PLP) is not added during the preparation, the apoenzyme prepared from glutamate decarboxylase contains no chromophore absorbing above 280 nm. Full enzyme activity and the original holoenzyme spectrum are restored by the addition of PLP alone. A 340 nm-absorbing band, similar to that which prompted analysis for PQQ, is produced by exposure of the enzyme to solutions of PLP.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
De Biase D,Maras B,John RAdoi
10.1016/0014-5793(91)80097-msubject
Has Abstractpub_date
1991-01-14 00:00:00pages
120-2issue
1eissn
0014-5793issn
1873-3468journal_volume
278pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Adrenal medullary homogenates and chromaffin granule membranes were separated by SDS-polyacrylamide gel electrophoresis and GTP-binding proteins detected using [alpha-32P]GTP binding to nitrocellulose blots. Four GTP-binding polypeptides of 24, 22, 20 and 18 kDa were routinely found in medullary homogenates and all we...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80204-2
更新日期:1989-03-13 00:00:00
abstract::Incubation of rhodanese with hche aperonins GroEL and GroES (1:2 GroEL14:GroES7 molar ratio) under functional and steady state conditions for ATP leads to the formation of a high proportion of rhodanese-bound symmetric complexes (GroEL14(GroES7)2), as revealed by native electrophoresis. Aliquots of such samples were o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00186-5
更新日期:1997-03-24 00:00:00
abstract::Gene duplication provides genetic material required for functional diversification. An interesting example is the amyloid precursor protein (APP) protein family. The APP gene family has experienced both expansion and contraction during evolution. The three mammalian members have been studied quite extensively in combi...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.05.026
更新日期:2013-06-27 00:00:00
abstract::BSP-30K is a major acidic glycoprotein of bovine seminal plasma. It displays heparin-, gelatin-, and phospholipid-binding activities. BSP-30K binds to spermatozoa upon ejaculation and is thought to play a role in sperm capacitation. We have determined its amino acid sequence, disulfide bonds, and 0-glycosylation sites...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01310-5
更新日期:1996-12-09 00:00:00
abstract::Human uncoupling protein 3 (UCP3) has two RNA transcripts that arise from the differential processing of the same gene product. One encodes the full length protein (UCP3L) while the other encodes a truncated version (UCP3S) lacking the sixth membrane spanning domain. The roles of the two isoforms are not known, but a ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01688-9
更新日期:2000-01-14 00:00:00
abstract::Integral cGMP-dependent currents as well as activity of single cGMP-activated channels in plasma membrane of rod outer segment (ROS) were recorded using the patch-clamp method. The dependence of integral currents on cGMP concentration is shown to be bell-shaped. Decrease in cGMP-dependent conductance at high cGMP conc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80089-2
更新日期:1990-01-15 00:00:00
abstract::Prominin-1 (CD133) is a cholesterol-interacting pentaspan membrane protein concentrated in plasma membrane protrusions. In epithelial cells, notably neuroepithelial stem cells, prominin-1 is found in microvilli, the primary cilium and the midbody. These three types of apical membrane protrusions are subject to remodel...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.01.050
更新日期:2010-05-03 00:00:00
abstract::Ghrelin is a novel gastrointestinal peptide that stimulates growth hormone secretion, food intake, and body weight gain. Increased ghrelin secretion has been reported in such negative energy states as starvation and low body weight. We investigated the dynamics of ghrelin in rats with streptozotocin-induced diabetes, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00306-5
更新日期:2003-04-24 00:00:00
abstract::Mutual arrangement of histone H1 molecules in chromatin extended in low salt-EDTA buffer and additionally in the presence of urea was studied by means of reversible cross-linking combined with chymotryptic digestion. In the chromatins tested, the chymotryptic halves of H1 were cross-linked in all possible combinations...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81164-8
更新日期:1983-10-31 00:00:00
abstract::A PCR-based subtractive cloning procedure was used to identify genes expressed at higher levels in the pancreatic beta cell line betaTC1, as compared to the pancreatic alpha cell line alphaTC1. One of the clones isolated by this procedure corresponded to the regulatory subunit (RIalpha) of protein kinase A (PKA). Usin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00186-0
更新日期:1998-03-20 00:00:00
abstract::The vasopressin precursor is composed of 3 domains in line, namely vasopressin, MSEL-neurophysin and a glycopeptide referred to as copeptin, which are separated during the processing. In guinea pig neurohypophysis, the precursor is partially processed so that a two-domain fragment, MSEL-neurophysin--copeptin, can be f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80320-9
更新日期:1986-03-03 00:00:00
abstract::The metabolite-modulated association of a fraction of hexokinase to mitochondria in brain is well documented, however, the involvement of other non-mitochondrial components in the binding of the hexokinase is controversial. Now we present evidence that the hexokinase binds both tubulin and microtubules in brain in vit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03136-2
更新日期:2001-11-30 00:00:00
abstract::Branched-chain 2-oxoacid dehydrogenase complex has been purified to near homogeneity by a simple, rapid procedure. The final product contains endogenous kinase activity capable of phosphorylating and inactivating the complex. Phosphorylation continues after complete inactivation, indicating the possibility of several ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81115-6
更新日期:1983-06-27 00:00:00
abstract::By sequence analysis we show that the U104 domain found in the UNC104 subfamily of kinesins is a forkhead homology-associated domain (FHA). A combination of limited proteolysis, mass spectroscopy, and physicochemical analysis define this domain as a genuine autonomously folding domain. Our data show that the FHA domai...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02310-3
更新日期:2000-12-15 00:00:00
abstract::In hepatocytes from control rats, the ureogenic action of epinephrine is mainly mediated through alpha 1-adrenoceptors and the effect is independent of the presence of extracellular calcium. In hepatocytes from adrenalectomized rats, both alpha 1- and beta-adrenoceptors are involved in the action of epinephrine. Furth...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80118-0
更新日期:1984-01-30 00:00:00
abstract::The number of proteins found in body fluids and at cell surfaces, which are known to display carbohydrate-binding properties, continues to increase rapidly. In these proteins, in addition to a domain associated with lectin properties, one or more, non-lectin domains are present. It is possible that binding of sugar re...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(89)80689-1
更新日期:1989-06-19 00:00:00
abstract::As yet, the physiological significance of hydroxylation of anandamide and linoleoyl amides is unknown. Therefore, we investigated whether hydroxylation of ODNHEtOH and ODNH2 influences their binding abilities to the CB-1 receptor and whether it alters their reactivity towards a fatty acid amide hydrolase (FAAH) from r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01148-4
更新日期:1997-10-06 00:00:00
abstract::Activated B61.SF.1 and CTLL-2 T lymphocyte clones which are strictly dependent on interleukin-2 (IL-2) for growth were used to study the activation of Na+/K+-ATPase. 50% of [3H]thymidine maximal incorporation was obtained when the extracellular concentration of Na+ or K+ was reduced to 50 or 2 mM, respectively. 'Quies...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80980-2
更新日期:1986-10-06 00:00:00
abstract::There are two common forms of NRH-quinone oxidoreductase 2 (NQO2) in the human population resulting from SNP rs1143684. One has phenylalanine at position 47 (NQO2-F47) and the other leucine (NQO2-L47). Using recombinant proteins, we show that these variants have similar steady state kinetic parameters, although NQO2-L...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.02.063
更新日期:2014-05-02 00:00:00
abstract::Chloride intracellular channel proteins (CLICs) are distinct from most ion channels in that they have both soluble and integral membrane forms. CLICs are highly conserved in chordates, with six vertebrate paralogues. CLIC-like proteins are found in other metazoans. CLICs form channels in artificial bilayers in a proce...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.01.027
更新日期:2010-05-17 00:00:00
abstract::The possibility that the phospholipid-N-methyltransferases from yeast are capable of acting upon a phospholipid substrate, localized in a different membrane than in which the enzymes reside ('trans-catalysis' hypothesis), was investigated using cho2 and opi3 gene disruptant strains, which are defective in phosphatidyl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02298-6
更新日期:2002-02-27 00:00:00
abstract::Several blood-feeding organisms, including the malaria parasite detoxify haem released from host haemoglobin by conversion to the insoluble crystalline ferriprotoporphyrin IX dimer known as haemozoin. To date the mechanism of haemozoin formation has remained unknown, although lipids or proteins have been suggested to ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.043
更新日期:2006-09-18 00:00:00
abstract::Data from a number of model systems support a role for proteolysis in apoptotic cell death. Using immature rat thymocytes, we demonstrate that the inhibitors N-tosyl-L-lysyl chloromethylketone (TLCK) and N-tosyl-L-phenylalanyl chloromethylketone (TPCK) have very different effects on apoptosis. TLCK inhibits apoptosis ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01367-a
更新日期:1995-01-09 00:00:00
abstract::A cell-free system derived from carrot cell cytosol extract has been developed for reassembling nuclear structure around the added demembranated sperm chromatin of Xenopus. Morphological evidence suggests that reassembled nuclei display the typical characteristics of normal eukaryotic nuclei, such as double-layered nu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01938-4
更新日期:2000-09-01 00:00:00
abstract::Single-pass sequences were obtained from the 5'-ends of a total of 1794 rose petal cDNA clones. Cluster analysis identified 242 groups of sequences and 635 singletons indicating that the database represents a total of 877 genes. Putative functions could be assigned to 1151 of the transcripts. Expression analysis indic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02413-4
更新日期:2002-03-27 00:00:00
abstract::The ADP(Mg2+)-deactivated oligomycin-sensitive F1-F0 ATPase of coupled submitochondrial particles treated with the substoichiometric amount of oligomycin was studied to test whether ATP synthesis and hydrolysis proceed in either direction through the same intermediates. The initial rates of ATP hydrolysis, oxidative p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00487-t
更新日期:1995-06-05 00:00:00
abstract::We have shown by electrophoretic mobility shift assays that the nucleotide sequence CE-LPH1, centred at position -49 with respect to the transcription start site of the rat gene encoding intestinal lactase-phlorizin hydrolase, interacts in vitro with nuclear proteins present in the jejunum of suckling animals. Protein...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01026-9
更新日期:1994-10-10 00:00:00
abstract::Chronic neuroleptic treatment induces an increase in the density of D2 dopamine receptors in the striatum. The effect of prolonged administration of haloperidol on mRNA levels of the short (D2S) and long (D2L) isoforms of the D2 dopamine receptor and the D3 dopamine receptor in different brain regions was examined. Ha...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80385-4
更新日期:1994-02-14 00:00:00
abstract::Transcriptional co-activators, co-repressors and chromatin remodeling machines are essential elements in the transcriptional programs directed by the master adipogenic transcription factor PPARgamma. Many of these components have orthologs in other organisms, where they play roles in development and pattern formation,...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.05.030
更新日期:2010-08-04 00:00:00
abstract::The fluorescent Ca2+ indicator Fura 2 was used to monitor Ca2+ release induced by the Ins(1,4,5)P3-mobilizing agonist angiotensin II (Ag II), caffeine and 2,5-di-(tert-butyl)-1,4-benzohydroquinone (tuBHQ), in intact bovine adrenal chromaffin cells. Under low external Ca2+ conditions, tuBHQ, Ag II and caffeine elicited...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81057-f
更新日期:1991-09-09 00:00:00