Abstract:
:We report the first evidence of a mitochondrial NO synthase (mtNOS) in bird skeletal muscle. In vitro, mtNOS activity stimulated by L-arginine reduced intermyofibrillar mitochondrial oxygen uptake and ATP synthesis rates, stimulated endogenous H(2)O(2) generation, but had no effect on oxidative phosphorylation efficiency. Arginine-induced effects were fully reversed by L-NAME, a known NOS inhibitor. When ducklings were cold exposed for 4 weeks, muscle mitochondria displayed an increased state 3 respiration, a reduced H(2)O(2) generation but no significant alteration in mtNOS activity. We conclude that mtNOS is expressed in avian skeletal muscle.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rey B,Roussel D,Teulier L,Eyenga P,Degletagne C,Belouze M,Duchamp Cdoi
10.1016/j.febslet.2010.11.032subject
Has Abstractpub_date
2011-01-03 00:00:00pages
173-7issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(10)00937-3journal_volume
585pub_type
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