Abstract:
:The F(1)F(0) ATP synthase has been purified from the hyperthermophilic eubacterium Aquifex aeolicus and characterized. Its subunits have been identified by MALDI-mass spectrometry through peptide mass fingerprinting and MS/MS. It contains the canonical subunits alpha, beta, gamma, delta and epsilon of F(1) and subunits a and c of F(0). Two versions of the b subunit were found, which show a low sequence homology to each other. Most likely they form a heterodimer. An electron microscopic single particle analysis revealed clear structural details, including two stalks connecting F(1) and F(0). In several orientations the central stalk appears to be tilted and/or kinked. It is unclear whether there is a direct connection between the peripheral stalk and the delta subunit.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Peng G,Bostina M,Radermacher M,Rais I,Karas M,Michel Hdoi
10.1016/j.febslet.2006.09.062subject
Has Abstractpub_date
2006-10-30 00:00:00pages
5934-40issue
25eissn
0014-5793issn
1873-3468pii
S0014-5793(06)01184-7journal_volume
580pub_type
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