Abstract:
:Poly-ADP ribosylation of nuclear proteins is activated when poly(ADP-ribose) polymerase (PARP), a nuclear zinc-finger enzyme, binds to single-strand DNA breaks. To understand how the signal emerging from its DNA-binding domain (DBD) bound to such breaks is transduced to its catalytic domain, the structure-function relationship of the DBD was investigated. We have used mutagenesis by the polymerase chain reaction (PCR) to generate a random library of PARP mutants. In this work, we describe the identification of catalytically inactive mutants bearing single point mutations, located outside the two zinc fingers in the DBD, that have conserved their full capacity to bind DNA. The results obtained demonstrate that the DNA-dependent activation of PARP requires not only a capacity to bind DNA but also a number of crucial residues to maintain a conformation of the domain necessary to transfer an 'activation signal' to the catalytic domain.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Trucco C,Flatter E,Fribourg S,de Murcia G,Ménissier-de Murcia Jdoi
10.1016/s0014-5793(96)01347-6subject
Has Abstractpub_date
1996-12-16 00:00:00pages
313-6issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(96)01347-6journal_volume
399pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Transforming growth factors-beta (TGFbeta1, 2 and 3) are secreted in a complex with their propeptides (latency-associated peptide 1 (LAP1), 2 and 3). TGFbeta signaling requires the dissociation of LAP and TGFbeta, a process termed latent TGFbeta activation. This process is a critical but incompletely understood step i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03280-x
更新日期:2002-01-30 00:00:00
abstract::Recent studies have shown that p21-activated kinase 1 (Pak1) phosphorylates estrogen receptor-alpha (ER alpha) at Ser 305 and also promotes its transactivation function. Here, we sought to investigate whether substitution of serine 305 in ER with glutamic acid (ER alpha-S305E), which mimics the phosphorylation state, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.071
更新日期:2004-06-04 00:00:00
abstract::A number of cDNA clones in summary encoding 700 amino acid residues from the N-end of rat liver elongation factor 2 (EF-2) and including 49 nucleotides of the 5'-untranslated mRNA region have been obtained. EF-2 cDNA clones were isolated from gradually constructed small (1000-5000 clones) specific cDNA libraries using...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80447-8
更新日期:1989-05-08 00:00:00
abstract::The amyloid precursor protein (APP) is proteolytically processed by β- and γ-secretases to release amyloid-β peptide (Aβ), the main component found in senile plaques of Alzheimer's disease (AD) patient brains. Alternatively, APP can be cleaved within the Aβ sequence by α-secretase, thus precluding the generation of Aβ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.05.048
更新日期:2011-07-21 00:00:00
abstract::In a previous study [(1987) J. Mol. Biol. 194, 663-673], we isolated ten insertion/deletion mutants (indels) of the maltose binding protein for which the maltose binding constant was only a little or not at all affected. In this paper, we have localized these mutations in the recently solved three-dimensional structur...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(93)81409-s
更新日期:1993-06-28 00:00:00
abstract::The verprolin family of proteins, WIP, CR16 and WIRE/WICH, has emerged as critical regulators of cytoskeletal organisation in vertebrate cells. The founding father of the family, verprolin, was originally identified in budding yeast and later shown to be needed for actin polymerisation during polarised growth and duri...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.08.053
更新日期:2005-10-10 00:00:00
abstract::We have examined the formation of DNA triple helices between the oligonucleotides T8XT8 (X = A,C,G,T) and DNA fragments containing the target sequences A8XA8.T8YT8 (X = T,C,G; Y = A,G,C), by DNase I footprinting. We find that A8GA8.T8CT8 yields a footprint with T8CT8 and shows a weaker interaction with T17 and T8GT8. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80510-2
更新日期:1993-10-11 00:00:00
abstract::The yeast PCK1 gene coding for phosphoenolpyruvate carboxykinase (PEPCK) was isolated by functional complementation of pck1 strains from S. cerevisiae. Only one copy of the gene was found per haploid yeast genome. An RNA of about 2 kb which hybridized with a DNA probe internal to the PCK1 gene was found only in cells ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81682-5
更新日期:1989-12-04 00:00:00
abstract::It was determined that flavan-3-ols and procyanidins have an inhibitory effect on angiotensin I converting enzyme (ACE) activity, and the effect was dependent on the number of epicatechin units forming the procyanidin. The inhibition by flavan-3-ols and procyanidins was competitive with the two substrates assayed: N-h...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01355-3
更新日期:2003-12-18 00:00:00
abstract::As an attempt to investigate the dynamic interactions between plasma serine protease, coagulation factor VIIa (VIIa) and its cofactor, tissue factor (TF), we performed normal mode analysis (NMA) of the complex of VIIa with soluble TF (the extracellular part of TF; sTF). We compared fluctuations of Calpha atoms of VIIa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01564-1
更新日期:1999-12-10 00:00:00
abstract::Exposing a macrophage-like murine cell line to copper-oxidised low-density lipoprotein led to DNA fragmentation which was inhibited by the putative Ca2+/Mg2+ endonuclease inhibitor, zinc sulphate. DNA fragmentation preceded loss of membrane impermeability. These results suggest that apoptosis may be a mechanism of mac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80635-8
更新日期:1993-10-18 00:00:00
abstract::Incubating either Chinese hamster ovary (CHO) cells or 3T3-L1 adipocytes with insulin increased the phosphorylation of the eIF-4E-binding protein, PHAS-I. Insulin also activated p70S6K and the Erk-1 and Erk-2 isoforms of mitogen-activated protein kinase (MAP kinase). However, the concentrations of the hormone needed t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00500-0
更新日期:1997-06-09 00:00:00
abstract::We have identified and characterized a genomic DNA fragment containing the coding sequences corresponding to the human gamma-glutamyltransferase type 1 mRNA. The coding part of the gene spans over 16 kb and comprises 12 exons and 11 introns exhibiting a similar organization as for the mouse and rat GGT genes. The exon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00950-8
更新日期:1998-08-28 00:00:00
abstract::Currently described substrates of the bacterial Tat protein transport system are directed for export by signal peptides containing a pair of invariant arginine residues. The signal peptide of the TtrB subunit of Salmonella enterica tetrathionate reductase contains a single arginine residue but is nevertheless able to ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02428-0
更新日期:2001-05-18 00:00:00
abstract::The effects of ribosomal protein L5 on the conformation of 5 S rRNA in the 5 S rRNA-protein L5 complex extracted from rat liver ribosomes have been studied by laser Raman spectroscopy. A comparison of the spectra shows small protein-induced conformational changes in the 5 S rRNA, but most of the base-paired regions ap...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)80622-4
更新日期:1983-05-08 00:00:00
abstract::Electron microscopy of mammalian smooth muscle myosin rods showed them to be 153 +/- 7 nm (SD) long, and to bend sharply (greater than 90 degrees) but infrequently, and pH independently (range 6.5-9.5), at a single site 45 +/- 4 nm from one end of the molecule. Light meromyosin (LMM) preparations were 99 +/- 10 nm lon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80940-0
更新日期:1984-10-15 00:00:00
abstract::The sequence of the 18 S rRNA of Tenebrio molitor is reported. A detailed secondary structure model for eukaryotic small subunit rRNAs is proposed. The model comprises 48 universal helices that eukaryotic and prokaryotic small subunit rRNAs have in common, plus a number of helices in areas of variable secondary struct...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80398-3
更新日期:1988-05-09 00:00:00
abstract::Inhibition of mammalian DNA topoisomerase I by phospholipids was investigated using purified enzyme. Acidic phospholipids inhibited the DNA relaxation activity of topoisomerase I whereas neutral phospholipid, phosphatidylethanolamine, did not. Accumulation of a protein-DNA cleavable complex, an intermediate which is k...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80658-6
更新日期:1990-02-12 00:00:00
abstract::Casein kinase-II (CK-II) belongs to the protein kinases recognizing serine/threonine in proximity to acidic residues in protein substrates. Crystallography and mutagenesis studies on the cAMP-dependent protein kinase (PKA) disclosed that glutamic acid-170 (E170), is important for interaction of substrates with the enz...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01190-7
更新日期:1994-12-05 00:00:00
abstract::The human ether-a-go-go-related gene (herg) encodes a K+ current (I(HERG)) which plays a fundamental role in heart excitability and in neurons by contributing to action potential repolarization and to spike-frequency adaptation, respectively. In this paper we show that I(HERG), recorded in neuroblastoma cells and guin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01444-6
更新日期:1998-11-27 00:00:00
abstract::Uroguanylin, a member of the guanylin peptide family, is a novel peptide regulator for intestinal salt and water transport. A cDNA encoding a precursor for rat uroguanylin was cloned from a rat jejunum cDNA library and sequenced. The precursor was 106 amino acids long and included a 21 residue putative signal peptide ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01235-5
更新日期:1996-12-02 00:00:00
abstract::Integral cGMP-dependent currents as well as activity of single cGMP-activated channels in plasma membrane of rod outer segment (ROS) were recorded using the patch-clamp method. The dependence of integral currents on cGMP concentration is shown to be bell-shaped. Decrease in cGMP-dependent conductance at high cGMP conc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80089-2
更新日期:1990-01-15 00:00:00
abstract::The oxidoreductase ERp57 is a component of the major histocompatibility complex (MHC) class I peptide-loading complex. ERp57 can interact directly with MHC class I molecules, however, little is known about which of the cysteine residues within the MHC class I molecule are relevant to this interaction. MHC class I mole...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.04.034
更新日期:2007-05-15 00:00:00
abstract::The amplification of hTERT was detected in glioma tissues, although telomerase activity was not always found within these specimens. The aim of this study was to correlate the level of hTERT transcription with telomerase activity in two glioma age groups. hTERT was significantly transcribed at similar copy numbers in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.04.027
更新日期:2008-05-28 00:00:00
abstract::Binding of vitronectin (VN) to Neisseria gonorrhoeae expressing the heparan sulfate proteoglycan (HSPG) specific Opa50 protein was recently shown to trigger bacterial internalization into distinct epithelial cell lines. We have investigated the role of VN-binding integrin receptors and protein kinase C (PKC) in VN-tri...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00144-6
更新日期:1998-03-06 00:00:00
abstract::Serotonin, a known neurotransmitter, also functions as an angiokine to promote angiogenesis. The majority of serotonin in the human body is stored in platelets, and platelet aggregation leads to significant release of serotonin in thrombotic tumor environment. We have investigated serotonin signaling in human endothel...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.05.047
更新日期:2012-07-30 00:00:00
abstract::A one-electron reductive metabolism of 1,2-dibromoethane (DBE) is described that gives rise to a free radical intermediate, which can be stabilized by a spin trapping agent and detected by electron spin resonance spectroscopy. Using rat liver microsomes or isolated hepatocytes from phenobarbitone pretreated animals, u...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80964-8
更新日期:1983-08-22 00:00:00
abstract::Despite significant increases in circulating concentrations of lipid fuels (triacylglycerol, non-esterified fatty acids (NEFA) and ketone bodies) in late-pregnant rats sampled in the fed (absorptive) state, cardiac and skeletal muscle active pyruvate dehydrogenase (PDHa) activities remained comparable with those obser...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80091-8
更新日期:1993-04-26 00:00:00
abstract::Adiponectin is increasingly recognized as a potential therapeutic agent for the treatment of diabetes and other metabolic diseases. It circulates in plasma as homotrimers and higher-order oliogomers of homotrimers. To facilitate the production of active recombinant adiponectin as a therapeutic tool, we designed a sing...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.02.024
更新日期:2012-03-23 00:00:00
abstract::In Alzheimer's disease and related dementias, human tau protein aggregates into paired helical filaments and neurofibrillary tangles. However, such tau aggregates have not yet been demonstrated in transgenic mouse models of the disease. One of the possible explanations would be that mouse tau has different properties ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00522-0
更新日期:1999-05-14 00:00:00