Modulation of the alpha 1A Ca2+ channel by beta subunits at physiological Ca2+ concentration.

abstract：:Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the nar...

journal_title：FEBS letters

authors： Roux M,Champeil P

更新日期：1984-06-11 00:00:00

abstract：:Proline residues are rarely found in the three most C-terminal positions of bacterial signal peptides, and have never been found in position +1 immediately following the cleavage site. It was recently shown that a Pro+1 mutation in the E. coli maltose binding protein precursor not only prevents cleavage of the signal ...

journal_title：FEBS letters

authors： Nilsson I,von Heijne G

更新日期：1992-03-16 00:00:00

abstract：:The rat synapse associated protein SAP90 is a member of a superfamily of potential guanylate kinases localized at cell-cell contact sites. This superfamily includes the synapse associated protein SAP97, a close relative of SAP90, the Drosophila tumor suppressor gene product dlg-Ap, the mammalian zonula occludens prote...

journal_title：FEBS letters

authors： Kistner U,Garner CC,Linial M

更新日期：1995-02-13 00:00:00

abstract：:Acaryochloris marina is a prochlorophyte-like cyanobacterium containing both phycobilins and chlorophyll d as light harvesting pigments. We show that the chlorophyll d light harvesting system, composed of Pcb proteins, functionally associates with the photosystem II (PSII) reaction center (RC) core to form a giant sup...

journal_title：FEBS letters

authors： Chen M,Bibby TS,Nield J,Larkum AW,Barber J

更新日期：2005-02-14 00:00:00

abstract：:LUSH is a soluble odorant-binding protein of the fruit fly Drosophila melanogaster. Mutants not expressing this protein have been reported to lack the avoidance behaviour, exhibited by wild type flies, to high concentrations of ethanol. Very recently, the three-dimensional structure of LUSH complexed with short-chain ...

journal_title：FEBS letters

authors： Zhou JJ,Zhang GA,Huang W,Birkett MA,Field LM,Pickett JA,Pelosi P

更新日期：2004-01-30 00:00:00

abstract：:A full-length cDNA coding for troponin-C isolated from an adult rabbit fast skeletal muscle library has been sequenced and expressed in an E. coli host. The amino acid sequence derived from the coding region agrees with the published protein sequence except that the first two residues are reversed. The expressed prote...

journal_title：FEBS letters

authors： Chen Q,Taljanidisz J,Sarkar S,Tao T,Gergely J

更新日期：1988-02-08 00:00:00

abstract：:It has been suggested that alpha-dystroglycan links the dystrophin-associated protein complex and extracellular matrix and that the absence of dystrophin and alpha-dystroglycan in Duchenne muscular dystrophy (DMD) may lead to the breakdown of this linkage. In the present study, myotubes from DMD patients and murine X-...

journal_title：FEBS letters

authors： Angoli D,Corona P,Baresi R,Mora M,Wanke E

更新日期：1997-05-26 00:00:00

abstract：:Antigen-induced B cell activation requires mobilization of the Ca(2+) second messenger. This process is associated with the subcellular relocalization of signal effector proteins of the B cell antigen receptor such as the adaptor protein SLP65. Here we describe a broadly applicable live cell imaging method to simultan...

journal_title：FEBS letters

authors： Junek S,Engelke M,Schild D,Wienands J

更新日期：2012-05-21 00:00:00

abstract：:Prominin-1 (CD133) is a cholesterol-interacting pentaspan membrane protein concentrated in plasma membrane protrusions. In epithelial cells, notably neuroepithelial stem cells, prominin-1 is found in microvilli, the primary cilium and the midbody. These three types of apical membrane protrusions are subject to remodel...

journal_title：FEBS letters

authors： Corbeil D,Marzesco AM,Wilsch-Bräuninger M,Huttner WB

更新日期：2010-05-03 00:00:00

abstract：:Specific binding sites for 3,3'-T2 can be detected in swollen and osmotically treated mitochondria (OTM) from normal and hypothyroid rat liver. In hypothyroid animals, maximal values of binding were obtained at 0 degrees C while values were lower at 37 degrees C and no specific binding could be observed at 60 degrees ...

journal_title：FEBS letters

authors： Lanni A,Moreno M,Horst C,Lombardi A,Goglia F

更新日期：1994-09-05 00:00:00

abstract：:It had been shown previously by heterologous expression in Schizosaccharomyces pombe, that the two monosaccharide/H+ symporters HUP1 and HUP2 of Chlorella kessleri differ significantly concerning their substrate specificity: HUP1 transports predominantly D-glucose while HUP2 prefers D-galactose. Several chimeric trans...

journal_title：FEBS letters

authors： Will A,Tanner W

更新日期：1996-02-26 00:00:00

abstract：:Almost all eukaryotic mRNAs acquire a poly(A) tail at the 3'-end by a concerted RNA processing event: cleavage and polyadenylation. The canonical PAP, PAPα, was considered the only nuclear PAP involved in general polyadenylation of mRNAs. A phosphoinositide-modulated nuclear PAP, Star-PAP, was then reported to regulat...

journal_title：FEBS letters

authors： Laishram RS

更新日期：2014-06-27 00:00:00

abstract：:Calpain, a Ca2+-dependent biomodulator, alters the properties of substrate proteins by cleaving them at a limited number of specific sites. Recent studies of the structure-function relationship of calpain and X-ray analysis of its Ca2+-binding domain have revealed hitherto unknown features of the regulation of calpain...

journal_title：FEBS letters

authors： Suzuki K,Sorimachi H

更新日期：1998-08-14 00:00:00

abstract：:The kinetics of complexing inactivation at identical enzyme and inhibitor concentrations were analyzed and the equations of product generation were derived when the free enzyme concentration is great, larger or smaller than the dissociation constant of inhibitor, K(I). The kinetic constants can be obtained by fitting ...

journal_title：FEBS letters

authors： Wang MH,Zhao KY

更新日期：1997-08-04 00:00:00

abstract：:Tyrosyl radicals can catalyze LDL oxidation. In addition to their LDL oxidizing ability, superoxide (O2.-)/nitric oxide (NO.) generate phenoxyl radicals when reacting with tyrosine. Therefore we tested if tyrosine can act as a pro-oxidant in O2.-/NO.-initiated LDL oxidation. When LDL was exposed to O2.-/NO., tyrosine ...

journal_title：FEBS letters

authors： Kapiotis S,Hermann M,Held I,Mühl A,Gmeiner B

更新日期：1997-06-09 00:00:00

abstract：:Phosphorylations catalyzed by cAMP-dependent protein kinase are essential for sperm motility, and type II cAMP-dependent protein kinase in mature sperm has been shown to be firmly bound to the flagellum via the regulatory subunit, RII. The present study documents high-levelled expression of a human, testis-specific RI...

journal_title：FEBS letters

authors： Oyen O,Myklebust F,Scott JD,Hansson V,Jahnsen T

更新日期：1989-03-27 00:00:00

abstract：:The NK-lysin derived peptide NK-2 is a potent antibacterial, but non-toxic to a human keratinocyte cell line and of low hemolytic activity. Its target selectivity is based upon a strong binding preference to membranes containing anionic phospholipids, which are normally not found on the surface of human cells. Here, w...

journal_title：FEBS letters

authors： Schröder-Borm H,Bakalova R,Andrä J

更新日期：2005-11-07 00:00:00

abstract：:A monoclonal antibody (Z1H01) for an oligopeptide carrying an azobenzene group, was prepared under conditions where the azobenzene group is in the trans form. The antibody bound the hapten peptide effectively when the hapten peptide is in the trans form (K = 5 x 10(7) M-1), but the antibody released the hapten under i...

journal_title：FEBS letters

authors： Harada M,Sisido M,Hirose J,Nakanishi M

更新日期：1991-07-29 00:00:00

abstract：:An alpha beta heterodimer of the F1-ATPase of Rhodospirillum rubrum was isolated by extraction of chromatophores with LiCl. Each alpha beta heterodimer contains one tightly bound ADP, which is released upon removal of medium Mg2+. The dimer can be reversibly dissociated by removal of Mg(2+)-ions. The alpha beta hetero...

journal_title：FEBS letters

authors： Andralojc PJ,Harris DA

更新日期：1992-09-28 00:00:00

abstract：:Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under p...

journal_title：FEBS letters

authors： Nakajima Y,Furuichi Y,Biswas KK,Hashiguchi T,Kawahara K,Yamaji K,Uchimura T,Izumi Y,Maruyama I

更新日期：2006-01-23 00:00:00

abstract：:The substrate specificity of purified PKC-alpha, -beta and -gamma has been investigated. A series of synthetic peptides based upon the sequence surrounding serine-7 in glycogen synthase were generated and used to determine the basic residue requirements of these PKC isotypes. While PKC-alpha and -beta are indistinguis...

journal_title：FEBS letters

authors： Marais RM,Nguyen O,Woodgett JR,Parker PJ

更新日期：1990-12-17 00:00:00

abstract：:Antibodies have been prepared against the 2-oxoglutarate transport proteins purified from bovine heart and rat liver mitochondria. The anti-heart antiserum cross-reacts with the 2-oxoglutarate carrier (OGC) from beef, pig, rat and rabbit heart, but not with the OGC from liver of the same animals. Conversely, the anti-...

journal_title：FEBS letters

authors： Zara V,De Benedittis R,Ragan CI,Palmieri F

更新日期：1990-04-24 00:00:00

abstract：:Abstract Hyperhomocysteinemia has recently been identified as an important risk factor for Alzheimer's disease (AD). One of the potential mechanisms underlying harmful effects of homocysteine (Hcy) is site-specific acylation of proteins at lysine residues by homocysteine thiolactone (HCTL). The accumulation of amyloid...

journal_title：FEBS letters

authors： Khodadadi S,Riazi GH,Ahmadian S,Hoveizi E,Karima O,Aryapour H

更新日期：2012-01-20 00:00:00

abstract：:Antithrombin III Hamilton is a structural variant of antithrombin III (AT-III) with normal heparin affinity but impaired serine protease inhibitory activity. The molecular defect of AT-III-Hamilton is a substitution of threonine for alanine at amino acid residue 382. Recently it has been shown that both plasma-derived...

journal_title：FEBS letters

authors： Austin RC,Rachubinski RA,Blajchman MA

更新日期：1991-03-25 00:00:00

abstract：:Jasmonic acid (JA) and its derivatives are well-characterized signaling molecules in plant defense and development, but the site of their localization within plant tissue is entirely unknown. To address the question whether applied JA accumulates extracellularly or intracellularly, leaves of tomato and barley were fed...

journal_title：FEBS letters

authors： Bücking H,Förster H,Stenzel I,Miersch O,Hause B

更新日期：2004-03-26 00:00:00

abstract：:Antimycin, 2-nonyl-4-hydroxyquinoline N-oxide and funiculosin induce O.2(-) generation by submitochondrial particles oxidizing succinate, whereas KCN, mucidin, myxothiazol or 2,3-dimercaptopropanol inhibit O.2(-) generation. Thenoyltrifluoroacetone does not induce superoxide production by itself but slightly stimulate...

journal_title：FEBS letters

authors： Ksenzenko M,Konstantinov AA,Khomutov GB,Tikhonov AN,Ruuge EK

更新日期：1983-05-02 00:00:00

abstract：:The covalent conjugation of oligonucleotides to antibody Fab' fragments was optimized by using oligonucleotides modified with a hexaethylene linker arm bearing three amino groups. One oligonucleotide was coupled to antibody of one specificity and a complementary oligonucleotide to antibody of a second specificity. The...

journal_title：FEBS letters

authors： Chaudri ZN,Bartlet-Jones M,Panayotou G,Klonisch T,Roitt IM,Lund T,Delves PJ

更新日期：1999-04-30 00:00:00

abstract：:Fatty acids induce apoptosis in primary astrocytes by enhancing ceramide synthesis de novo. The possible role of the AMP-activated protein kinase (AMPK) in the control of apoptosis was studied in this model. Long-term stimulation of AMPK with 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) prevented apoptosis. A...

journal_title：FEBS letters

authors： Blázquez C,Geelen MJ,Velasco G,Guzmán M

更新日期：2001-02-02 00:00:00

abstract：:Induction of differentiation in HL-60 and U-937 leukemic cell lines, resulted in 1.5-10-fold increase in 45Ca2+ uptake. The increased 45Ca2+ uptake in the differentiating cells was inhibited by verapamil, cromolyn and amiloride. Elevation in Ca2+ uptake in differentiating cells was also demonstrated using the fluoresc...

journal_title：FEBS letters

authors： Rephaeli A,Aviram A,Englender T,Shaklai M

更新日期：1988-08-29 00:00:00

abstract：:The mammalian Ptdlns 3-kinase is shown to be inhibited by low nanomolar concentrations of demethoxyviridin, an antifungal agent structurally related to wortmannin. The inhibitory potency of both compounds could be observed in purified Ptdlns 3-kinase whether or not the regulatory subunit (p85 alpha) was present, sugge...

journal_title：FEBS letters

authors： Woscholski R,Kodaki T,McKinnon M,Waterfield MD,Parker PJ

更新日期：1994-04-04 00:00:00